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Biocompatibility and Angiogenic Effect of Chitosan/Graphene Oxide Hydrogel Scaffolds on EPCs

Angiogenesis in the field of tissue engineering has attracted significant attention. Graphene oxide has become a promising nanomaterial in tissue engineering for its unique biochemical properties. Therefore, herein, a series of chitosan (CS)/graphene oxide (GO) hydrogel scaffolds were synthesized by...

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Detalles Bibliográficos
Autores principales: Zhang, Lifang, Li, Xinping, Shi, Congying, Ran, Gaoying, Peng, Yuting, Zeng, Shuguang, He, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154284/
https://www.ncbi.nlm.nih.gov/pubmed/34113384
http://dx.doi.org/10.1155/2021/5594370
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author Zhang, Lifang
Li, Xinping
Shi, Congying
Ran, Gaoying
Peng, Yuting
Zeng, Shuguang
He, Yan
author_facet Zhang, Lifang
Li, Xinping
Shi, Congying
Ran, Gaoying
Peng, Yuting
Zeng, Shuguang
He, Yan
author_sort Zhang, Lifang
collection PubMed
description Angiogenesis in the field of tissue engineering has attracted significant attention. Graphene oxide has become a promising nanomaterial in tissue engineering for its unique biochemical properties. Therefore, herein, a series of chitosan (CS)/graphene oxide (GO) hydrogel scaffolds were synthesized by crosslinking CS and GO at different concentrations (0.1, 0.5, and 1.0 wt.%) using genipin. Compared with the CS hydrogel scaffolds, the CS/GO hydrogel scaffolds have a better network structure and mechanical strength. Then, we used endothelial progenitor cells (EPCs) extracted from human umbilical cord blood and cocultured these EPCs with the as-prepared scaffolds. The scaffolds with 0.1 and 0.5 wt.%GO showed no considerable cytotoxicity, could promote the proliferation of EPCs and tube formation, and upregulated the expressions of CD34, VEGF, MMP9, and SDF-1 in EPCs compared to the case of the scaffold with 1.0 wt.%GO. This study shows that the addition of graphene oxide improves the structure of chitosan hydrogel and enhances the proliferation activity and angiogenic capacity of EPCs.
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spelling pubmed-81542842021-06-09 Biocompatibility and Angiogenic Effect of Chitosan/Graphene Oxide Hydrogel Scaffolds on EPCs Zhang, Lifang Li, Xinping Shi, Congying Ran, Gaoying Peng, Yuting Zeng, Shuguang He, Yan Stem Cells Int Research Article Angiogenesis in the field of tissue engineering has attracted significant attention. Graphene oxide has become a promising nanomaterial in tissue engineering for its unique biochemical properties. Therefore, herein, a series of chitosan (CS)/graphene oxide (GO) hydrogel scaffolds were synthesized by crosslinking CS and GO at different concentrations (0.1, 0.5, and 1.0 wt.%) using genipin. Compared with the CS hydrogel scaffolds, the CS/GO hydrogel scaffolds have a better network structure and mechanical strength. Then, we used endothelial progenitor cells (EPCs) extracted from human umbilical cord blood and cocultured these EPCs with the as-prepared scaffolds. The scaffolds with 0.1 and 0.5 wt.%GO showed no considerable cytotoxicity, could promote the proliferation of EPCs and tube formation, and upregulated the expressions of CD34, VEGF, MMP9, and SDF-1 in EPCs compared to the case of the scaffold with 1.0 wt.%GO. This study shows that the addition of graphene oxide improves the structure of chitosan hydrogel and enhances the proliferation activity and angiogenic capacity of EPCs. Hindawi 2021-05-18 /pmc/articles/PMC8154284/ /pubmed/34113384 http://dx.doi.org/10.1155/2021/5594370 Text en Copyright © 2021 Lifang Zhang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhang, Lifang
Li, Xinping
Shi, Congying
Ran, Gaoying
Peng, Yuting
Zeng, Shuguang
He, Yan
Biocompatibility and Angiogenic Effect of Chitosan/Graphene Oxide Hydrogel Scaffolds on EPCs
title Biocompatibility and Angiogenic Effect of Chitosan/Graphene Oxide Hydrogel Scaffolds on EPCs
title_full Biocompatibility and Angiogenic Effect of Chitosan/Graphene Oxide Hydrogel Scaffolds on EPCs
title_fullStr Biocompatibility and Angiogenic Effect of Chitosan/Graphene Oxide Hydrogel Scaffolds on EPCs
title_full_unstemmed Biocompatibility and Angiogenic Effect of Chitosan/Graphene Oxide Hydrogel Scaffolds on EPCs
title_short Biocompatibility and Angiogenic Effect of Chitosan/Graphene Oxide Hydrogel Scaffolds on EPCs
title_sort biocompatibility and angiogenic effect of chitosan/graphene oxide hydrogel scaffolds on epcs
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154284/
https://www.ncbi.nlm.nih.gov/pubmed/34113384
http://dx.doi.org/10.1155/2021/5594370
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