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miR-96-5p: A potential diagnostic marker for gestational diabetes mellitus

MicroRNAs play important roles in gestational diabetes mellitus (GDM), and this study aimed to elucidate the clinical significance of miR-96-5p in diagnosing GDM. There are 123 pregnant women diagnosed with GDM and 123 healthy pregnant women were enrolled as control participants. Placenta and plasma...

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Detalles Bibliográficos
Autores principales: Yu, Xinyang, Liu, Zhengfei, Fang, Jie, Qi, Hongbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Lippincott Williams & Wilkins 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154478/
https://www.ncbi.nlm.nih.gov/pubmed/34032694
http://dx.doi.org/10.1097/MD.0000000000025808
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author Yu, Xinyang
Liu, Zhengfei
Fang, Jie
Qi, Hongbo
author_facet Yu, Xinyang
Liu, Zhengfei
Fang, Jie
Qi, Hongbo
author_sort Yu, Xinyang
collection PubMed
description MicroRNAs play important roles in gestational diabetes mellitus (GDM), and this study aimed to elucidate the clinical significance of miR-96-5p in diagnosing GDM. There are 123 pregnant women diagnosed with GDM and 123 healthy pregnant women were enrolled as control participants. Placenta and plasma samples from the patients and control participants were collected, and quantitative reverse transcription polymerase chain reaction (RT-qPCR) was performed to determine miR-96-5p expression levels. Moreover, a receiver operating characteristic (ROC) curve was established to evaluate the significance of miR-96-5p in diagnosing GDM. HRT-8/SVneo trophoblasts were cultured under high glucose conditions and treated with miR-96-5p mimics, and cell viability was examined. miR-96-5p levels were significantly decreased in both the placenta and plasma samples of patients with GDM. The ROC curve indicated that miR-96-5p can serve as a diagnostic biomarker for GDM with high sensitivity and specificity. Moreover, miR-96-5p levels were markedly low under high glucose conditions, and the overexpression of miR-96-5p increased the viability, respectively, of trophoblasts in vitro. miR-96-5p may participate in the pathogenesis of GDM by exerting effects on the viability of trophoblasts.
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spelling pubmed-81544782021-05-29 miR-96-5p: A potential diagnostic marker for gestational diabetes mellitus Yu, Xinyang Liu, Zhengfei Fang, Jie Qi, Hongbo Medicine (Baltimore) 5600 MicroRNAs play important roles in gestational diabetes mellitus (GDM), and this study aimed to elucidate the clinical significance of miR-96-5p in diagnosing GDM. There are 123 pregnant women diagnosed with GDM and 123 healthy pregnant women were enrolled as control participants. Placenta and plasma samples from the patients and control participants were collected, and quantitative reverse transcription polymerase chain reaction (RT-qPCR) was performed to determine miR-96-5p expression levels. Moreover, a receiver operating characteristic (ROC) curve was established to evaluate the significance of miR-96-5p in diagnosing GDM. HRT-8/SVneo trophoblasts were cultured under high glucose conditions and treated with miR-96-5p mimics, and cell viability was examined. miR-96-5p levels were significantly decreased in both the placenta and plasma samples of patients with GDM. The ROC curve indicated that miR-96-5p can serve as a diagnostic biomarker for GDM with high sensitivity and specificity. Moreover, miR-96-5p levels were markedly low under high glucose conditions, and the overexpression of miR-96-5p increased the viability, respectively, of trophoblasts in vitro. miR-96-5p may participate in the pathogenesis of GDM by exerting effects on the viability of trophoblasts. Lippincott Williams & Wilkins 2021-05-28 /pmc/articles/PMC8154478/ /pubmed/34032694 http://dx.doi.org/10.1097/MD.0000000000025808 Text en Copyright © 2021 the Author(s). Published by Wolters Kluwer Health, Inc. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License 4.0 (CCBY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/licenses/by/4.0 (https://creativecommons.org/licenses/by/4.0/)
spellingShingle 5600
Yu, Xinyang
Liu, Zhengfei
Fang, Jie
Qi, Hongbo
miR-96-5p: A potential diagnostic marker for gestational diabetes mellitus
title miR-96-5p: A potential diagnostic marker for gestational diabetes mellitus
title_full miR-96-5p: A potential diagnostic marker for gestational diabetes mellitus
title_fullStr miR-96-5p: A potential diagnostic marker for gestational diabetes mellitus
title_full_unstemmed miR-96-5p: A potential diagnostic marker for gestational diabetes mellitus
title_short miR-96-5p: A potential diagnostic marker for gestational diabetes mellitus
title_sort mir-96-5p: a potential diagnostic marker for gestational diabetes mellitus
topic 5600
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154478/
https://www.ncbi.nlm.nih.gov/pubmed/34032694
http://dx.doi.org/10.1097/MD.0000000000025808
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