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Environmental DNA detection of an invasive ant species (Linepithema humile) from soil samples
Alien ant species (Formicidae, Hymenoptera) cause serious damage worldwide. Early detection of invasion and rapid management are significant for controlling these species. However, these attempts are sometimes hindered by the need for direct detection techniques, such as capture, visual observation,...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154996/ https://www.ncbi.nlm.nih.gov/pubmed/34040019 http://dx.doi.org/10.1038/s41598-021-89993-9 |
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author | Yasashimoto, Tetsu Sakata, Masayuki K. Sakita, Tomoya Nakajima, Satoko Ozaki, Mamiko Minamoto, Toshifumi |
author_facet | Yasashimoto, Tetsu Sakata, Masayuki K. Sakita, Tomoya Nakajima, Satoko Ozaki, Mamiko Minamoto, Toshifumi |
author_sort | Yasashimoto, Tetsu |
collection | PubMed |
description | Alien ant species (Formicidae, Hymenoptera) cause serious damage worldwide. Early detection of invasion and rapid management are significant for controlling these species. However, these attempts are sometimes hindered by the need for direct detection techniques, such as capture, visual observation, or morphological identification. In this study, we demonstrated that environmental DNA (eDNA) analysis can be used as a monitoring tool for alien ants using Linepithema humile (Argentine ant), one of the most invasive ants, as a model species. We designed a new real-time PCR assay specific to L. humile and successfully detected eDNA from the surface soil. The reliability of eDNA analysis was substantiated by comparing eDNA detection results with traditional survey results. Additionally, we examined the relationship between eDNA concentration and distance from nests and trails. Our results support the effectiveness of eDNA for alien ant monitoring and suggest that this new method could improve our ability to detect invasive ant species. |
format | Online Article Text |
id | pubmed-8154996 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-81549962021-05-27 Environmental DNA detection of an invasive ant species (Linepithema humile) from soil samples Yasashimoto, Tetsu Sakata, Masayuki K. Sakita, Tomoya Nakajima, Satoko Ozaki, Mamiko Minamoto, Toshifumi Sci Rep Article Alien ant species (Formicidae, Hymenoptera) cause serious damage worldwide. Early detection of invasion and rapid management are significant for controlling these species. However, these attempts are sometimes hindered by the need for direct detection techniques, such as capture, visual observation, or morphological identification. In this study, we demonstrated that environmental DNA (eDNA) analysis can be used as a monitoring tool for alien ants using Linepithema humile (Argentine ant), one of the most invasive ants, as a model species. We designed a new real-time PCR assay specific to L. humile and successfully detected eDNA from the surface soil. The reliability of eDNA analysis was substantiated by comparing eDNA detection results with traditional survey results. Additionally, we examined the relationship between eDNA concentration and distance from nests and trails. Our results support the effectiveness of eDNA for alien ant monitoring and suggest that this new method could improve our ability to detect invasive ant species. Nature Publishing Group UK 2021-05-26 /pmc/articles/PMC8154996/ /pubmed/34040019 http://dx.doi.org/10.1038/s41598-021-89993-9 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Yasashimoto, Tetsu Sakata, Masayuki K. Sakita, Tomoya Nakajima, Satoko Ozaki, Mamiko Minamoto, Toshifumi Environmental DNA detection of an invasive ant species (Linepithema humile) from soil samples |
title | Environmental DNA detection of an invasive ant species (Linepithema humile) from soil samples |
title_full | Environmental DNA detection of an invasive ant species (Linepithema humile) from soil samples |
title_fullStr | Environmental DNA detection of an invasive ant species (Linepithema humile) from soil samples |
title_full_unstemmed | Environmental DNA detection of an invasive ant species (Linepithema humile) from soil samples |
title_short | Environmental DNA detection of an invasive ant species (Linepithema humile) from soil samples |
title_sort | environmental dna detection of an invasive ant species (linepithema humile) from soil samples |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154996/ https://www.ncbi.nlm.nih.gov/pubmed/34040019 http://dx.doi.org/10.1038/s41598-021-89993-9 |
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