Cargando…

Environmental DNA detection of an invasive ant species (Linepithema humile) from soil samples

Alien ant species (Formicidae, Hymenoptera) cause serious damage worldwide. Early detection of invasion and rapid management are significant for controlling these species. However, these attempts are sometimes hindered by the need for direct detection techniques, such as capture, visual observation,...

Descripción completa

Detalles Bibliográficos
Autores principales: Yasashimoto, Tetsu, Sakata, Masayuki K., Sakita, Tomoya, Nakajima, Satoko, Ozaki, Mamiko, Minamoto, Toshifumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154996/
https://www.ncbi.nlm.nih.gov/pubmed/34040019
http://dx.doi.org/10.1038/s41598-021-89993-9
_version_ 1783699114507108352
author Yasashimoto, Tetsu
Sakata, Masayuki K.
Sakita, Tomoya
Nakajima, Satoko
Ozaki, Mamiko
Minamoto, Toshifumi
author_facet Yasashimoto, Tetsu
Sakata, Masayuki K.
Sakita, Tomoya
Nakajima, Satoko
Ozaki, Mamiko
Minamoto, Toshifumi
author_sort Yasashimoto, Tetsu
collection PubMed
description Alien ant species (Formicidae, Hymenoptera) cause serious damage worldwide. Early detection of invasion and rapid management are significant for controlling these species. However, these attempts are sometimes hindered by the need for direct detection techniques, such as capture, visual observation, or morphological identification. In this study, we demonstrated that environmental DNA (eDNA) analysis can be used as a monitoring tool for alien ants using Linepithema humile (Argentine ant), one of the most invasive ants, as a model species. We designed a new real-time PCR assay specific to L. humile and successfully detected eDNA from the surface soil. The reliability of eDNA analysis was substantiated by comparing eDNA detection results with traditional survey results. Additionally, we examined the relationship between eDNA concentration and distance from nests and trails. Our results support the effectiveness of eDNA for alien ant monitoring and suggest that this new method could improve our ability to detect invasive ant species.
format Online
Article
Text
id pubmed-8154996
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-81549962021-05-27 Environmental DNA detection of an invasive ant species (Linepithema humile) from soil samples Yasashimoto, Tetsu Sakata, Masayuki K. Sakita, Tomoya Nakajima, Satoko Ozaki, Mamiko Minamoto, Toshifumi Sci Rep Article Alien ant species (Formicidae, Hymenoptera) cause serious damage worldwide. Early detection of invasion and rapid management are significant for controlling these species. However, these attempts are sometimes hindered by the need for direct detection techniques, such as capture, visual observation, or morphological identification. In this study, we demonstrated that environmental DNA (eDNA) analysis can be used as a monitoring tool for alien ants using Linepithema humile (Argentine ant), one of the most invasive ants, as a model species. We designed a new real-time PCR assay specific to L. humile and successfully detected eDNA from the surface soil. The reliability of eDNA analysis was substantiated by comparing eDNA detection results with traditional survey results. Additionally, we examined the relationship between eDNA concentration and distance from nests and trails. Our results support the effectiveness of eDNA for alien ant monitoring and suggest that this new method could improve our ability to detect invasive ant species. Nature Publishing Group UK 2021-05-26 /pmc/articles/PMC8154996/ /pubmed/34040019 http://dx.doi.org/10.1038/s41598-021-89993-9 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Yasashimoto, Tetsu
Sakata, Masayuki K.
Sakita, Tomoya
Nakajima, Satoko
Ozaki, Mamiko
Minamoto, Toshifumi
Environmental DNA detection of an invasive ant species (Linepithema humile) from soil samples
title Environmental DNA detection of an invasive ant species (Linepithema humile) from soil samples
title_full Environmental DNA detection of an invasive ant species (Linepithema humile) from soil samples
title_fullStr Environmental DNA detection of an invasive ant species (Linepithema humile) from soil samples
title_full_unstemmed Environmental DNA detection of an invasive ant species (Linepithema humile) from soil samples
title_short Environmental DNA detection of an invasive ant species (Linepithema humile) from soil samples
title_sort environmental dna detection of an invasive ant species (linepithema humile) from soil samples
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154996/
https://www.ncbi.nlm.nih.gov/pubmed/34040019
http://dx.doi.org/10.1038/s41598-021-89993-9
work_keys_str_mv AT yasashimototetsu environmentaldnadetectionofaninvasiveantspecieslinepithemahumilefromsoilsamples
AT sakatamasayukik environmentaldnadetectionofaninvasiveantspecieslinepithemahumilefromsoilsamples
AT sakitatomoya environmentaldnadetectionofaninvasiveantspecieslinepithemahumilefromsoilsamples
AT nakajimasatoko environmentaldnadetectionofaninvasiveantspecieslinepithemahumilefromsoilsamples
AT ozakimamiko environmentaldnadetectionofaninvasiveantspecieslinepithemahumilefromsoilsamples
AT minamototoshifumi environmentaldnadetectionofaninvasiveantspecieslinepithemahumilefromsoilsamples