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HBO1 overexpression is important for hepatocellular carcinoma cell growth
Hepatocellular carcinoma (HCC) is a common primary liver malignancy lacking effective molecularly-targeted therapies. HBO1 (lysine acetyltransferase 7/KAT7) is a member of MYST histone acetyltransferase family. Its expression and potential function in HCC are studied. We show that HBO1 mRNA and prot...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155027/ https://www.ncbi.nlm.nih.gov/pubmed/34039960 http://dx.doi.org/10.1038/s41419-021-03818-1 |
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author | Zhong, Wenhui Liu, Heping Deng, Li Chen, Guohua Liu, Yubin |
author_facet | Zhong, Wenhui Liu, Heping Deng, Li Chen, Guohua Liu, Yubin |
author_sort | Zhong, Wenhui |
collection | PubMed |
description | Hepatocellular carcinoma (HCC) is a common primary liver malignancy lacking effective molecularly-targeted therapies. HBO1 (lysine acetyltransferase 7/KAT7) is a member of MYST histone acetyltransferase family. Its expression and potential function in HCC are studied. We show that HBO1 mRNA and protein expression is elevated in human HCC tissues and HCC cells. HBO1 expression is however low in cancer-surrounding normal liver tissues and hepatocytes. In HepG2 and primary human HCC cells, shRNA-induced HBO1 silencing or CRISPR/Cas9-induced HBO1 knockout potently inhibited cell viability, proliferation, migration, and invasion, while provoking mitochondrial depolarization and apoptosis induction. Conversely, ectopic overexpression of HBO1 by a lentiviral construct augmented HCC cell proliferation, migration and invasion. In vivo, xenografts-bearing HBO1-KO HCC cells grew significantly slower than xenografts with control HCC cells in severe combined immunodeficient mice. These results suggest HBO1 overexpression is important for HCC cell progression. |
format | Online Article Text |
id | pubmed-8155027 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-81550272021-06-10 HBO1 overexpression is important for hepatocellular carcinoma cell growth Zhong, Wenhui Liu, Heping Deng, Li Chen, Guohua Liu, Yubin Cell Death Dis Article Hepatocellular carcinoma (HCC) is a common primary liver malignancy lacking effective molecularly-targeted therapies. HBO1 (lysine acetyltransferase 7/KAT7) is a member of MYST histone acetyltransferase family. Its expression and potential function in HCC are studied. We show that HBO1 mRNA and protein expression is elevated in human HCC tissues and HCC cells. HBO1 expression is however low in cancer-surrounding normal liver tissues and hepatocytes. In HepG2 and primary human HCC cells, shRNA-induced HBO1 silencing or CRISPR/Cas9-induced HBO1 knockout potently inhibited cell viability, proliferation, migration, and invasion, while provoking mitochondrial depolarization and apoptosis induction. Conversely, ectopic overexpression of HBO1 by a lentiviral construct augmented HCC cell proliferation, migration and invasion. In vivo, xenografts-bearing HBO1-KO HCC cells grew significantly slower than xenografts with control HCC cells in severe combined immunodeficient mice. These results suggest HBO1 overexpression is important for HCC cell progression. Nature Publishing Group UK 2021-05-26 /pmc/articles/PMC8155027/ /pubmed/34039960 http://dx.doi.org/10.1038/s41419-021-03818-1 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Zhong, Wenhui Liu, Heping Deng, Li Chen, Guohua Liu, Yubin HBO1 overexpression is important for hepatocellular carcinoma cell growth |
title | HBO1 overexpression is important for hepatocellular carcinoma cell growth |
title_full | HBO1 overexpression is important for hepatocellular carcinoma cell growth |
title_fullStr | HBO1 overexpression is important for hepatocellular carcinoma cell growth |
title_full_unstemmed | HBO1 overexpression is important for hepatocellular carcinoma cell growth |
title_short | HBO1 overexpression is important for hepatocellular carcinoma cell growth |
title_sort | hbo1 overexpression is important for hepatocellular carcinoma cell growth |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155027/ https://www.ncbi.nlm.nih.gov/pubmed/34039960 http://dx.doi.org/10.1038/s41419-021-03818-1 |
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