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Development of a yeast cell surface display method using the SpyTag/SpyCatcher system

Yeast cell surface display (YSD) has been used to engineer various proteins, including antibodies. Directed evolution, which subjects a gene to iterative rounds of mutagenesis, selection and amplification, is useful for protein engineering. In vivo continuous mutagenesis, which continuously diversif...

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Detalles Bibliográficos
Autores principales: Kajiwara, Kaho, Aoki, Wataru, Koike, Naoki, Ueda, Mitsuyoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155107/
https://www.ncbi.nlm.nih.gov/pubmed/34040114
http://dx.doi.org/10.1038/s41598-021-90593-w