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TRIM71 Deficiency Causes Germ Cell Loss During Mouse Embryogenesis and Is Associated With Human Male Infertility

Mutations affecting the germline can result in infertility or the generation of germ cell tumors (GCT), highlighting the need to identify and characterize the genes controlling germ cell development. The RNA-binding protein and E3 ubiquitin ligase TRIM71 is essential for embryogenesis, and its expre...

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Autores principales: Torres-Fernández, Lucia A., Emich, Jana, Port, Yasmine, Mitschka, Sibylle, Wöste, Marius, Schneider, Simon, Fietz, Daniela, Oud, Manon S., Di Persio, Sara, Neuhaus, Nina, Kliesch, Sabine, Hölzel, Michael, Schorle, Hubert, Friedrich, Corinna, Tüttelmann, Frank, Kolanus, Waldemar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155544/
https://www.ncbi.nlm.nih.gov/pubmed/34055789
http://dx.doi.org/10.3389/fcell.2021.658966
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author Torres-Fernández, Lucia A.
Emich, Jana
Port, Yasmine
Mitschka, Sibylle
Wöste, Marius
Schneider, Simon
Fietz, Daniela
Oud, Manon S.
Di Persio, Sara
Neuhaus, Nina
Kliesch, Sabine
Hölzel, Michael
Schorle, Hubert
Friedrich, Corinna
Tüttelmann, Frank
Kolanus, Waldemar
author_facet Torres-Fernández, Lucia A.
Emich, Jana
Port, Yasmine
Mitschka, Sibylle
Wöste, Marius
Schneider, Simon
Fietz, Daniela
Oud, Manon S.
Di Persio, Sara
Neuhaus, Nina
Kliesch, Sabine
Hölzel, Michael
Schorle, Hubert
Friedrich, Corinna
Tüttelmann, Frank
Kolanus, Waldemar
author_sort Torres-Fernández, Lucia A.
collection PubMed
description Mutations affecting the germline can result in infertility or the generation of germ cell tumors (GCT), highlighting the need to identify and characterize the genes controlling germ cell development. The RNA-binding protein and E3 ubiquitin ligase TRIM71 is essential for embryogenesis, and its expression has been reported in GCT and adult mouse testes. To investigate the role of TRIM71 in mammalian germ cell embryonic development, we generated a germline-specific conditional Trim71 knockout mouse (cKO) using the early primordial germ cell (PGC) marker Nanos3 as a Cre-recombinase driver. cKO mice are infertile, with male mice displaying a Sertoli cell-only (SCO) phenotype which in humans is defined as a specific subtype of non-obstructive azoospermia characterized by the absence of germ cells in the seminiferous tubules. Infertility in male Trim71 cKO mice originates during embryogenesis, as the SCO phenotype was already apparent in neonatal mice. The in vitro differentiation of mouse embryonic stem cells (ESCs) into PGC-like cells (PGCLCs) revealed reduced numbers of PGCLCs in Trim71-deficient cells. Furthermore, TCam-2 cells, a human GCT-derived seminoma cell line which was used as an in vitro model for PGCs, showed proliferation defects upon TRIM71 knockdown. Additionally, in vitro growth competition assays, as well as proliferation assays with wild type and CRISPR/Cas9-generated TRIM71 mutant NCCIT cells showed that TRIM71 also promotes proliferation in this malignant GCT-derived non-seminoma cell line. Importantly, the PGC-specific markers BLIMP1 and NANOS3 were consistently downregulated in Trim71 KO PGCLCs, TRIM71 knockdown TCam-2 cells and TRIM71 mutant NCCIT cells. These data collectively support a role for TRIM71 in PGC development. Last, via exome sequencing analysis, we identified several TRIM71 variants in a cohort of infertile men, including a loss-of-function variant in a patient with an SCO phenotype. Altogether, our work reveals for the first time an association of TRIM71 deficiency with human male infertility, and uncovers further developmental roles for TRIM71 in the germline during mouse embryogenesis.
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spelling pubmed-81555442021-05-28 TRIM71 Deficiency Causes Germ Cell Loss During Mouse Embryogenesis and Is Associated With Human Male Infertility Torres-Fernández, Lucia A. Emich, Jana Port, Yasmine Mitschka, Sibylle Wöste, Marius Schneider, Simon Fietz, Daniela Oud, Manon S. Di Persio, Sara Neuhaus, Nina Kliesch, Sabine Hölzel, Michael Schorle, Hubert Friedrich, Corinna Tüttelmann, Frank Kolanus, Waldemar Front Cell Dev Biol Cell and Developmental Biology Mutations affecting the germline can result in infertility or the generation of germ cell tumors (GCT), highlighting the need to identify and characterize the genes controlling germ cell development. The RNA-binding protein and E3 ubiquitin ligase TRIM71 is essential for embryogenesis, and its expression has been reported in GCT and adult mouse testes. To investigate the role of TRIM71 in mammalian germ cell embryonic development, we generated a germline-specific conditional Trim71 knockout mouse (cKO) using the early primordial germ cell (PGC) marker Nanos3 as a Cre-recombinase driver. cKO mice are infertile, with male mice displaying a Sertoli cell-only (SCO) phenotype which in humans is defined as a specific subtype of non-obstructive azoospermia characterized by the absence of germ cells in the seminiferous tubules. Infertility in male Trim71 cKO mice originates during embryogenesis, as the SCO phenotype was already apparent in neonatal mice. The in vitro differentiation of mouse embryonic stem cells (ESCs) into PGC-like cells (PGCLCs) revealed reduced numbers of PGCLCs in Trim71-deficient cells. Furthermore, TCam-2 cells, a human GCT-derived seminoma cell line which was used as an in vitro model for PGCs, showed proliferation defects upon TRIM71 knockdown. Additionally, in vitro growth competition assays, as well as proliferation assays with wild type and CRISPR/Cas9-generated TRIM71 mutant NCCIT cells showed that TRIM71 also promotes proliferation in this malignant GCT-derived non-seminoma cell line. Importantly, the PGC-specific markers BLIMP1 and NANOS3 were consistently downregulated in Trim71 KO PGCLCs, TRIM71 knockdown TCam-2 cells and TRIM71 mutant NCCIT cells. These data collectively support a role for TRIM71 in PGC development. Last, via exome sequencing analysis, we identified several TRIM71 variants in a cohort of infertile men, including a loss-of-function variant in a patient with an SCO phenotype. Altogether, our work reveals for the first time an association of TRIM71 deficiency with human male infertility, and uncovers further developmental roles for TRIM71 in the germline during mouse embryogenesis. Frontiers Media S.A. 2021-05-13 /pmc/articles/PMC8155544/ /pubmed/34055789 http://dx.doi.org/10.3389/fcell.2021.658966 Text en Copyright © 2021 Torres-Fernández, Emich, Port, Mitschka, Wöste, Schneider, Fietz, Oud, Di Persio, Neuhaus, Kliesch, Hölzel, Schorle, Friedrich, Tüttelmann and Kolanus. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Torres-Fernández, Lucia A.
Emich, Jana
Port, Yasmine
Mitschka, Sibylle
Wöste, Marius
Schneider, Simon
Fietz, Daniela
Oud, Manon S.
Di Persio, Sara
Neuhaus, Nina
Kliesch, Sabine
Hölzel, Michael
Schorle, Hubert
Friedrich, Corinna
Tüttelmann, Frank
Kolanus, Waldemar
TRIM71 Deficiency Causes Germ Cell Loss During Mouse Embryogenesis and Is Associated With Human Male Infertility
title TRIM71 Deficiency Causes Germ Cell Loss During Mouse Embryogenesis and Is Associated With Human Male Infertility
title_full TRIM71 Deficiency Causes Germ Cell Loss During Mouse Embryogenesis and Is Associated With Human Male Infertility
title_fullStr TRIM71 Deficiency Causes Germ Cell Loss During Mouse Embryogenesis and Is Associated With Human Male Infertility
title_full_unstemmed TRIM71 Deficiency Causes Germ Cell Loss During Mouse Embryogenesis and Is Associated With Human Male Infertility
title_short TRIM71 Deficiency Causes Germ Cell Loss During Mouse Embryogenesis and Is Associated With Human Male Infertility
title_sort trim71 deficiency causes germ cell loss during mouse embryogenesis and is associated with human male infertility
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155544/
https://www.ncbi.nlm.nih.gov/pubmed/34055789
http://dx.doi.org/10.3389/fcell.2021.658966
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