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Influence of Environmental Temperature and Hormonal Stimulation on the In Vitro Sperm Maturation in Sterlet Acipenser ruthenus in Advance of the Spawning Season

SIMPLE SUMMARY: Sperm maturation (acquisition of the potential for motility and fertilization by morphologically developed spermatozoa) in sturgeons is atypical of ray-finned fishes: it occurs outside the testes during the transit of testicular spermatozoa through the kidneys into the Wolffian ducts...

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Autores principales: Dzyuba, Viktoriya, Cosson, Jacky, Papadaki, Maria, Mylonas, Constantinos C., Steinbach, Christoph, Rodina, Marek, Tučkova, Vladimira, Linhart, Otomar, Shelton, William L., Gela, David, Boryshpolets, Sergii, Dzyuba, Borys
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155876/
https://www.ncbi.nlm.nih.gov/pubmed/34063418
http://dx.doi.org/10.3390/ani11051417
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author Dzyuba, Viktoriya
Cosson, Jacky
Papadaki, Maria
Mylonas, Constantinos C.
Steinbach, Christoph
Rodina, Marek
Tučkova, Vladimira
Linhart, Otomar
Shelton, William L.
Gela, David
Boryshpolets, Sergii
Dzyuba, Borys
author_facet Dzyuba, Viktoriya
Cosson, Jacky
Papadaki, Maria
Mylonas, Constantinos C.
Steinbach, Christoph
Rodina, Marek
Tučkova, Vladimira
Linhart, Otomar
Shelton, William L.
Gela, David
Boryshpolets, Sergii
Dzyuba, Borys
author_sort Dzyuba, Viktoriya
collection PubMed
description SIMPLE SUMMARY: Sperm maturation (acquisition of the potential for motility and fertilization by morphologically developed spermatozoa) in sturgeons is atypical of ray-finned fishes: it occurs outside the testes during the transit of testicular spermatozoa through the kidneys into the Wolffian ducts. We recently developed a method in which testicular spermatozoa of sterlet Acipenser ruthenus are matured in vitro when incubated in seminal fluid derived from Wolffian duct sperm. In this study, we explored whether in vitro maturation of testicular spermatozoa depends on the environmental temperature and/or hormonal stimulation of spermiation. We studied spermatozoa motility parameters after in vitro maturation of testicular sperm, concentrations of sex steroid hormones and testis morphology in sterlet males at different stages of male preparation for spawning with and without hormonal induction of spermiation. The obtained data suggest that the ability of testicular spermatozoa to be matured was not related to the environmental temperature, while hormonal stimulation was an absolute requirement for optimal in vitro maturation. The use of in vitro matured testicular spermatozoa might have considerable potential in aquaculture or conservation programs, which can be realized in cases of accidental death of valuable broodstock or failure to obtain Wolffian duct sperm of high quality. ABSTRACT: Sturgeon sperm maturation occurs outside the testes during the transit of testicular spermatozoa (TS) through the kidneys and the Wolffian ducts. A method of in vitro TS maturation in sterlet Acipenser ruthenus was used to investigate the effects of temperature and hormonal stimulation of spermiation on the ability of TS to complete this process. Spermatozoa motility parameters after in vitro maturation of testicular sperm, concentrations of sex steroid hormones and testis morphology were studied in three groups of sterlet: (1) after overwintering in ponds (OW), (2) adapted to spawning temperature (ST), and (3) adapted to spawning temperature with hormonal induction of spermiation (ST-HI). Blood plasma concentrations of testosterone, 11-ketotestosterone and 17,20β-dihydroxy-pregnenolone increased significantly after hormonal induction of spermiation (group ST-HI). In all groups, TS were not motile. After in vitro sperm maturation, motility was up to 60% only in group ST-HI. The data suggest that the ability of TS to be matured in vitro was not related to the environmental temperature, while hormonal stimulation of spermiation during the spawning season was an absolute requirement for optimal in vitro maturation.
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spelling pubmed-81558762021-05-28 Influence of Environmental Temperature and Hormonal Stimulation on the In Vitro Sperm Maturation in Sterlet Acipenser ruthenus in Advance of the Spawning Season Dzyuba, Viktoriya Cosson, Jacky Papadaki, Maria Mylonas, Constantinos C. Steinbach, Christoph Rodina, Marek Tučkova, Vladimira Linhart, Otomar Shelton, William L. Gela, David Boryshpolets, Sergii Dzyuba, Borys Animals (Basel) Article SIMPLE SUMMARY: Sperm maturation (acquisition of the potential for motility and fertilization by morphologically developed spermatozoa) in sturgeons is atypical of ray-finned fishes: it occurs outside the testes during the transit of testicular spermatozoa through the kidneys into the Wolffian ducts. We recently developed a method in which testicular spermatozoa of sterlet Acipenser ruthenus are matured in vitro when incubated in seminal fluid derived from Wolffian duct sperm. In this study, we explored whether in vitro maturation of testicular spermatozoa depends on the environmental temperature and/or hormonal stimulation of spermiation. We studied spermatozoa motility parameters after in vitro maturation of testicular sperm, concentrations of sex steroid hormones and testis morphology in sterlet males at different stages of male preparation for spawning with and without hormonal induction of spermiation. The obtained data suggest that the ability of testicular spermatozoa to be matured was not related to the environmental temperature, while hormonal stimulation was an absolute requirement for optimal in vitro maturation. The use of in vitro matured testicular spermatozoa might have considerable potential in aquaculture or conservation programs, which can be realized in cases of accidental death of valuable broodstock or failure to obtain Wolffian duct sperm of high quality. ABSTRACT: Sturgeon sperm maturation occurs outside the testes during the transit of testicular spermatozoa (TS) through the kidneys and the Wolffian ducts. A method of in vitro TS maturation in sterlet Acipenser ruthenus was used to investigate the effects of temperature and hormonal stimulation of spermiation on the ability of TS to complete this process. Spermatozoa motility parameters after in vitro maturation of testicular sperm, concentrations of sex steroid hormones and testis morphology were studied in three groups of sterlet: (1) after overwintering in ponds (OW), (2) adapted to spawning temperature (ST), and (3) adapted to spawning temperature with hormonal induction of spermiation (ST-HI). Blood plasma concentrations of testosterone, 11-ketotestosterone and 17,20β-dihydroxy-pregnenolone increased significantly after hormonal induction of spermiation (group ST-HI). In all groups, TS were not motile. After in vitro sperm maturation, motility was up to 60% only in group ST-HI. The data suggest that the ability of TS to be matured in vitro was not related to the environmental temperature, while hormonal stimulation of spermiation during the spawning season was an absolute requirement for optimal in vitro maturation. MDPI 2021-05-15 /pmc/articles/PMC8155876/ /pubmed/34063418 http://dx.doi.org/10.3390/ani11051417 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dzyuba, Viktoriya
Cosson, Jacky
Papadaki, Maria
Mylonas, Constantinos C.
Steinbach, Christoph
Rodina, Marek
Tučkova, Vladimira
Linhart, Otomar
Shelton, William L.
Gela, David
Boryshpolets, Sergii
Dzyuba, Borys
Influence of Environmental Temperature and Hormonal Stimulation on the In Vitro Sperm Maturation in Sterlet Acipenser ruthenus in Advance of the Spawning Season
title Influence of Environmental Temperature and Hormonal Stimulation on the In Vitro Sperm Maturation in Sterlet Acipenser ruthenus in Advance of the Spawning Season
title_full Influence of Environmental Temperature and Hormonal Stimulation on the In Vitro Sperm Maturation in Sterlet Acipenser ruthenus in Advance of the Spawning Season
title_fullStr Influence of Environmental Temperature and Hormonal Stimulation on the In Vitro Sperm Maturation in Sterlet Acipenser ruthenus in Advance of the Spawning Season
title_full_unstemmed Influence of Environmental Temperature and Hormonal Stimulation on the In Vitro Sperm Maturation in Sterlet Acipenser ruthenus in Advance of the Spawning Season
title_short Influence of Environmental Temperature and Hormonal Stimulation on the In Vitro Sperm Maturation in Sterlet Acipenser ruthenus in Advance of the Spawning Season
title_sort influence of environmental temperature and hormonal stimulation on the in vitro sperm maturation in sterlet acipenser ruthenus in advance of the spawning season
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155876/
https://www.ncbi.nlm.nih.gov/pubmed/34063418
http://dx.doi.org/10.3390/ani11051417
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