Method for Rapid Analysis of Mutant RNA Polymerase Activity on Templates Containing Unnatural Nucleotides

Pairs of unnatural nucleotides are used to expand the genetic code and create artificial DNA or RNA templates. In general, an approach is used to engineer orthogonal systems capable of reading codons comprising artificial nucleotides; however, DNA and RNA polymerases capable of recognizing unnatural...

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Detalles Bibliográficos
Autores principales: Egorova, Tatiana, Shuvalova, Ekaterina, Mukba, Sabina, Shuvalov, Alexey, Kolosov, Peter, Alkalaeva, Elena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155940/
https://www.ncbi.nlm.nih.gov/pubmed/34069057
http://dx.doi.org/10.3390/ijms22105186

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