Detection of Antimicrobial Residues in Poultry Litter: Monitoring a Risk through a Selective and Sensitive HPLC–MS/MS Method

SIMPLE SUMMARY: After administration of antimicrobials, poultry excrete significant concentrations of antimicrobials through their droppings, which accumulate in the litter where poultry are housed. Poultry litter, which consists mainly of wood shavings, feathers, and droppings, is widely used as an agricultural fertilizer worldwide. The period that antimicrobials persist in agricultural soils could present various environmental and public health concerns. Thus, in this research, a method to identify different families of antimicrobials in poultry litter was developed. Results show that HPLC–MS/MS can reliably detect nine different compounds from three families of antimicrobials. This method was used to identify antimicrobials from commercial poultry flocks, providing a valuable and specific tool to monitor these residues in poultry litter prior to its use as an agricultural fertilizer. ABSTRACT: Tetracyclines, sulphonamides, and quinolones are families of antimicrobials (AMs) widely used in the poultry industry and can excrete up to 90% of AMs administrated, which accumulate in poultry litter. Worryingly, poultry litter is widely used as an agriculture fertilizer, contributing to the spread AMs residues in the environment. The aim of this research was to develop a method that could simultaneously identify and quantify three AMs families in poultry litter by high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS). Samples of AMs free poultry litter were used to validate the method according to 657/2002/EC and VICH GL49. Results indicate that limit of detection (LOD) ranged from 8.95 to 20.86 μg kg(−1), while limits of quantitation (LOQ) values were between 26.85 and 62.58 µg kg(−1) of tetracycline, 4-epi-tetracycline, oxytetracycline, 4-epi-oxytetracycline, enrofloxacin, ciprofloxacin, flumequine, sulfachloropyridazine, and sulfadiazine. Recoveries obtained ranged from 93 to 108%. The analysis of field samples obtained from seven commercial poultry flocks confirmed the adequacy of the method since it detected means concentrations ranging from 20 to 10,364 μg kg(−1). This provides us an accurate and reliable tool to monitor AMs residues in poultry litter and control its use as agricultural fertilizer.