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A Selective Ratiometric Fluorescent Probe for No-Wash Detection of PVC Microplastic

Microplastics (MP) are micrometric plastic particles present in drinking water, food and the environment that constitute an emerging pollutant and pose a menace to human health. Novel methods for the fast detection of these new contaminants are needed. Fluorescence-based detection exploits the use o...

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Autores principales: Caponetti, Valeria, Mavridi-Printezi, Alexandra, Cingolani, Matteo, Rampazzo, Enrico, Genovese, Damiano, Prodi, Luca, Fabbri, Daniele, Montalti, Marco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8156183/
https://www.ncbi.nlm.nih.gov/pubmed/34069160
http://dx.doi.org/10.3390/polym13101588
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author Caponetti, Valeria
Mavridi-Printezi, Alexandra
Cingolani, Matteo
Rampazzo, Enrico
Genovese, Damiano
Prodi, Luca
Fabbri, Daniele
Montalti, Marco
author_facet Caponetti, Valeria
Mavridi-Printezi, Alexandra
Cingolani, Matteo
Rampazzo, Enrico
Genovese, Damiano
Prodi, Luca
Fabbri, Daniele
Montalti, Marco
author_sort Caponetti, Valeria
collection PubMed
description Microplastics (MP) are micrometric plastic particles present in drinking water, food and the environment that constitute an emerging pollutant and pose a menace to human health. Novel methods for the fast detection of these new contaminants are needed. Fluorescence-based detection exploits the use of specific probes to label the MP particles. This method can be environmentally friendly, low-cost, easily scalable but also very sensitive and specific. Here, we present the synthesis and application of a new probe based on perylene-diimide (PDI), which can be prepared in a few minutes by a one-pot reaction using a conventional microwave oven and can be used for the direct detection of MP in water without any further treatment of the sample. The green fluorescence is strongly quenched in water at neutral pH because of the formation dimers. The ability of the probe to label MP was tested for polyvinyl chloride (PVC), polyethylene (PE), polyethylene terephthalate (PET), polypropylene (PP), polystyrene (PS), poly methyl methacrylate (PMMA) and polytetrafluoroethylene (PTFE). The probe showed considerable selectivity to PVC MP, which presented an intense red emission after staining. Interestingly, the fluorescence of the MP after labeling could be detected, under excitation with a blue diode, with a conventional CMOS color camera. Good selectivity was achieved analyzing the red to green fluorescence intensity ratio. UV–Vis absorption, steady-state and time-resolved fluorescence spectroscopy, fluorescence anisotropy, fluorescence wide-field and confocal laser scanning microscopy allowed elucidating the mechanism of the staining in detail.
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spelling pubmed-81561832021-05-28 A Selective Ratiometric Fluorescent Probe for No-Wash Detection of PVC Microplastic Caponetti, Valeria Mavridi-Printezi, Alexandra Cingolani, Matteo Rampazzo, Enrico Genovese, Damiano Prodi, Luca Fabbri, Daniele Montalti, Marco Polymers (Basel) Article Microplastics (MP) are micrometric plastic particles present in drinking water, food and the environment that constitute an emerging pollutant and pose a menace to human health. Novel methods for the fast detection of these new contaminants are needed. Fluorescence-based detection exploits the use of specific probes to label the MP particles. This method can be environmentally friendly, low-cost, easily scalable but also very sensitive and specific. Here, we present the synthesis and application of a new probe based on perylene-diimide (PDI), which can be prepared in a few minutes by a one-pot reaction using a conventional microwave oven and can be used for the direct detection of MP in water without any further treatment of the sample. The green fluorescence is strongly quenched in water at neutral pH because of the formation dimers. The ability of the probe to label MP was tested for polyvinyl chloride (PVC), polyethylene (PE), polyethylene terephthalate (PET), polypropylene (PP), polystyrene (PS), poly methyl methacrylate (PMMA) and polytetrafluoroethylene (PTFE). The probe showed considerable selectivity to PVC MP, which presented an intense red emission after staining. Interestingly, the fluorescence of the MP after labeling could be detected, under excitation with a blue diode, with a conventional CMOS color camera. Good selectivity was achieved analyzing the red to green fluorescence intensity ratio. UV–Vis absorption, steady-state and time-resolved fluorescence spectroscopy, fluorescence anisotropy, fluorescence wide-field and confocal laser scanning microscopy allowed elucidating the mechanism of the staining in detail. MDPI 2021-05-14 /pmc/articles/PMC8156183/ /pubmed/34069160 http://dx.doi.org/10.3390/polym13101588 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Caponetti, Valeria
Mavridi-Printezi, Alexandra
Cingolani, Matteo
Rampazzo, Enrico
Genovese, Damiano
Prodi, Luca
Fabbri, Daniele
Montalti, Marco
A Selective Ratiometric Fluorescent Probe for No-Wash Detection of PVC Microplastic
title A Selective Ratiometric Fluorescent Probe for No-Wash Detection of PVC Microplastic
title_full A Selective Ratiometric Fluorescent Probe for No-Wash Detection of PVC Microplastic
title_fullStr A Selective Ratiometric Fluorescent Probe for No-Wash Detection of PVC Microplastic
title_full_unstemmed A Selective Ratiometric Fluorescent Probe for No-Wash Detection of PVC Microplastic
title_short A Selective Ratiometric Fluorescent Probe for No-Wash Detection of PVC Microplastic
title_sort selective ratiometric fluorescent probe for no-wash detection of pvc microplastic
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8156183/
https://www.ncbi.nlm.nih.gov/pubmed/34069160
http://dx.doi.org/10.3390/polym13101588
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