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Carotenoid Nostoxanthin Production by Sphingomonas sp. SG73 Isolated from Deep Sea Sediment

Carotenoids are used commercially for dietary supplements, cosmetics, and pharmaceuticals because of their antioxidant activity. In this study, colored microorganisms were isolated from deep sea sediment that had been collected from Suruga Bay, Shizuoka, Japan. One strain was found to be a pure yell...

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Autores principales: Kikukawa, Hiroshi, Okaya, Takuma, Maoka, Takashi, Miyazaki, Masayuki, Murofushi, Keita, Kato, Takanari, Hirono-Hara, Yoko, Katsumata, Masahiro, Miyahara, Shoichi, Hara, Kiyotaka Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8156329/
https://www.ncbi.nlm.nih.gov/pubmed/34068940
http://dx.doi.org/10.3390/md19050274
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author Kikukawa, Hiroshi
Okaya, Takuma
Maoka, Takashi
Miyazaki, Masayuki
Murofushi, Keita
Kato, Takanari
Hirono-Hara, Yoko
Katsumata, Masahiro
Miyahara, Shoichi
Hara, Kiyotaka Y.
author_facet Kikukawa, Hiroshi
Okaya, Takuma
Maoka, Takashi
Miyazaki, Masayuki
Murofushi, Keita
Kato, Takanari
Hirono-Hara, Yoko
Katsumata, Masahiro
Miyahara, Shoichi
Hara, Kiyotaka Y.
author_sort Kikukawa, Hiroshi
collection PubMed
description Carotenoids are used commercially for dietary supplements, cosmetics, and pharmaceuticals because of their antioxidant activity. In this study, colored microorganisms were isolated from deep sea sediment that had been collected from Suruga Bay, Shizuoka, Japan. One strain was found to be a pure yellow carotenoid producer, and the strain was identified as Sphingomonas sp. (Proteobacteria) by 16S rRNA gene sequence analysis; members of this genus are commonly isolated from air, the human body, and marine environments. The carotenoid was identified as nostoxanthin ((2,3,2′,3′)-β,β-carotene-2,3,2′,3′-tetrol) by mass spectrometry (MS), MS/MS, and ultraviolet-visible absorption spectroscopy (UV-Vis). Nostoxanthin is a poly-hydroxy yellow carotenoid isolated from some photosynthetic bacteria, including some species of Cyanobacteria. The strain Sphingomonas sp. SG73 produced highly pure nostoxanthin of approximately 97% (area%) of the total carotenoid production, and the strain was halophilic and tolerant to 1.5-fold higher salt concentration as compared with seawater. When grown in 1.8% artificial sea salt, nostoxanthin production increased by 2.5-fold as compared with production without artificial sea salt. These results indicate that Sphingomonas sp. SG73 is an efficient producer of nostoxanthin, and the strain is ideal for carotenoid production using marine water because of its compatibility with sea salt.
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spelling pubmed-81563292021-05-28 Carotenoid Nostoxanthin Production by Sphingomonas sp. SG73 Isolated from Deep Sea Sediment Kikukawa, Hiroshi Okaya, Takuma Maoka, Takashi Miyazaki, Masayuki Murofushi, Keita Kato, Takanari Hirono-Hara, Yoko Katsumata, Masahiro Miyahara, Shoichi Hara, Kiyotaka Y. Mar Drugs Article Carotenoids are used commercially for dietary supplements, cosmetics, and pharmaceuticals because of their antioxidant activity. In this study, colored microorganisms were isolated from deep sea sediment that had been collected from Suruga Bay, Shizuoka, Japan. One strain was found to be a pure yellow carotenoid producer, and the strain was identified as Sphingomonas sp. (Proteobacteria) by 16S rRNA gene sequence analysis; members of this genus are commonly isolated from air, the human body, and marine environments. The carotenoid was identified as nostoxanthin ((2,3,2′,3′)-β,β-carotene-2,3,2′,3′-tetrol) by mass spectrometry (MS), MS/MS, and ultraviolet-visible absorption spectroscopy (UV-Vis). Nostoxanthin is a poly-hydroxy yellow carotenoid isolated from some photosynthetic bacteria, including some species of Cyanobacteria. The strain Sphingomonas sp. SG73 produced highly pure nostoxanthin of approximately 97% (area%) of the total carotenoid production, and the strain was halophilic and tolerant to 1.5-fold higher salt concentration as compared with seawater. When grown in 1.8% artificial sea salt, nostoxanthin production increased by 2.5-fold as compared with production without artificial sea salt. These results indicate that Sphingomonas sp. SG73 is an efficient producer of nostoxanthin, and the strain is ideal for carotenoid production using marine water because of its compatibility with sea salt. MDPI 2021-05-14 /pmc/articles/PMC8156329/ /pubmed/34068940 http://dx.doi.org/10.3390/md19050274 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kikukawa, Hiroshi
Okaya, Takuma
Maoka, Takashi
Miyazaki, Masayuki
Murofushi, Keita
Kato, Takanari
Hirono-Hara, Yoko
Katsumata, Masahiro
Miyahara, Shoichi
Hara, Kiyotaka Y.
Carotenoid Nostoxanthin Production by Sphingomonas sp. SG73 Isolated from Deep Sea Sediment
title Carotenoid Nostoxanthin Production by Sphingomonas sp. SG73 Isolated from Deep Sea Sediment
title_full Carotenoid Nostoxanthin Production by Sphingomonas sp. SG73 Isolated from Deep Sea Sediment
title_fullStr Carotenoid Nostoxanthin Production by Sphingomonas sp. SG73 Isolated from Deep Sea Sediment
title_full_unstemmed Carotenoid Nostoxanthin Production by Sphingomonas sp. SG73 Isolated from Deep Sea Sediment
title_short Carotenoid Nostoxanthin Production by Sphingomonas sp. SG73 Isolated from Deep Sea Sediment
title_sort carotenoid nostoxanthin production by sphingomonas sp. sg73 isolated from deep sea sediment
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8156329/
https://www.ncbi.nlm.nih.gov/pubmed/34068940
http://dx.doi.org/10.3390/md19050274
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