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Lupeol Accumulation Correlates with Auxin in the Epidermis of Castor

Lupeol, a natural lupane-type pentacyclic triterpene, possesses various pharmacological properties, and its production attracts attention. Significant quantities of lupeol are deposited on the castor aerial organ surface and are easily extractable as a predominant wax constituent. Thus, castor might...

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Autores principales: Li, Donghai, Pan, Cheng, Lu, Jianjun, Zaman, Wajid, Zhao, Huayan, Zhang, Jixing, Lü, Shiyou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8156332/
https://www.ncbi.nlm.nih.gov/pubmed/34067825
http://dx.doi.org/10.3390/molecules26102978
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author Li, Donghai
Pan, Cheng
Lu, Jianjun
Zaman, Wajid
Zhao, Huayan
Zhang, Jixing
Lü, Shiyou
author_facet Li, Donghai
Pan, Cheng
Lu, Jianjun
Zaman, Wajid
Zhao, Huayan
Zhang, Jixing
Lü, Shiyou
author_sort Li, Donghai
collection PubMed
description Lupeol, a natural lupane-type pentacyclic triterpene, possesses various pharmacological properties, and its production attracts attention. Significant quantities of lupeol are deposited on the castor aerial organ surface and are easily extractable as a predominant wax constituent. Thus, castor might be considered as a potential bioreactor for the production of lupeol. The lupeol biosynthesis pathway is well known, but how it is regulated remains largely unknown. Among large numbers of castor cultivars, we targeted one accession line (337) with high levels of lupeol on its stem surface and low levels thereof on its hypocotyl surface, implicating that lupeol synthesis is differentially regulated in the two organs. To explore the underlying mechanisms, we did comparative transcriptome analysis of the first internode of 337 stem and the upper hypocotyl. Our results show that large amounts of auxin-related genes are differentially expressed in both parts, implying some possible interactions between auxin and lupeol production. We also found that several auxin-responsive cis-elements are present in promoter regions of HMGR and LUS genes encoding two key enzymes involved in lupeol production. Furthermore, auxin treatments apparently induced the expression levels of RcHMGR and RcLUS. Furthermore, we observed that auxin treatment significantly increased lupeol contents, whereas inhibiting auxin transport led to an opposite phenotype. Our study reveals some relationships between hormone activity and lupeol synthesis and might provide a promising way for improving lupeol yields in castor.
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spelling pubmed-81563322021-05-28 Lupeol Accumulation Correlates with Auxin in the Epidermis of Castor Li, Donghai Pan, Cheng Lu, Jianjun Zaman, Wajid Zhao, Huayan Zhang, Jixing Lü, Shiyou Molecules Article Lupeol, a natural lupane-type pentacyclic triterpene, possesses various pharmacological properties, and its production attracts attention. Significant quantities of lupeol are deposited on the castor aerial organ surface and are easily extractable as a predominant wax constituent. Thus, castor might be considered as a potential bioreactor for the production of lupeol. The lupeol biosynthesis pathway is well known, but how it is regulated remains largely unknown. Among large numbers of castor cultivars, we targeted one accession line (337) with high levels of lupeol on its stem surface and low levels thereof on its hypocotyl surface, implicating that lupeol synthesis is differentially regulated in the two organs. To explore the underlying mechanisms, we did comparative transcriptome analysis of the first internode of 337 stem and the upper hypocotyl. Our results show that large amounts of auxin-related genes are differentially expressed in both parts, implying some possible interactions between auxin and lupeol production. We also found that several auxin-responsive cis-elements are present in promoter regions of HMGR and LUS genes encoding two key enzymes involved in lupeol production. Furthermore, auxin treatments apparently induced the expression levels of RcHMGR and RcLUS. Furthermore, we observed that auxin treatment significantly increased lupeol contents, whereas inhibiting auxin transport led to an opposite phenotype. Our study reveals some relationships between hormone activity and lupeol synthesis and might provide a promising way for improving lupeol yields in castor. MDPI 2021-05-17 /pmc/articles/PMC8156332/ /pubmed/34067825 http://dx.doi.org/10.3390/molecules26102978 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Donghai
Pan, Cheng
Lu, Jianjun
Zaman, Wajid
Zhao, Huayan
Zhang, Jixing
Lü, Shiyou
Lupeol Accumulation Correlates with Auxin in the Epidermis of Castor
title Lupeol Accumulation Correlates with Auxin in the Epidermis of Castor
title_full Lupeol Accumulation Correlates with Auxin in the Epidermis of Castor
title_fullStr Lupeol Accumulation Correlates with Auxin in the Epidermis of Castor
title_full_unstemmed Lupeol Accumulation Correlates with Auxin in the Epidermis of Castor
title_short Lupeol Accumulation Correlates with Auxin in the Epidermis of Castor
title_sort lupeol accumulation correlates with auxin in the epidermis of castor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8156332/
https://www.ncbi.nlm.nih.gov/pubmed/34067825
http://dx.doi.org/10.3390/molecules26102978
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