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Candida albicans/Macrophage Biointerface on Human and Porcine Decellularized Adipose Matrices
Macrophages, cells effective in sensing, internalizing and killing Candida albicans, are intertwined with the extracellular matrix (ECM) through different signals, which include the release of specific cytokines. Due to the importance of these interactions, the employment of in vitro models mimickin...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8156393/ https://www.ncbi.nlm.nih.gov/pubmed/34067785 http://dx.doi.org/10.3390/jof7050392 |
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author | Cicuéndez, Mónica Casarrubios, Laura Feito, María José Madarieta, Iratxe Garcia-Urkia, Nerea Murua, Olatz Olalde, Beatriz Briz, Nerea Diez-Orejas, Rosalía Portolés, María Teresa |
author_facet | Cicuéndez, Mónica Casarrubios, Laura Feito, María José Madarieta, Iratxe Garcia-Urkia, Nerea Murua, Olatz Olalde, Beatriz Briz, Nerea Diez-Orejas, Rosalía Portolés, María Teresa |
author_sort | Cicuéndez, Mónica |
collection | PubMed |
description | Macrophages, cells effective in sensing, internalizing and killing Candida albicans, are intertwined with the extracellular matrix (ECM) through different signals, which include the release of specific cytokines. Due to the importance of these interactions, the employment of in vitro models mimicking a fungal infection scenario is essential to evaluate the ECM effects on the macrophage response. In this work, we have analyzed the effects of human and porcine decellularized adipose matrices (DAMs), obtained by either enzymatic or organic solvent treatment, on the macrophage/Candida albicans interface. The present study has allowed us to detect differences on the activation of macrophages cultured on either human- or porcine-derived DAMs, evidencing changes in the macrophage actin cytoskeleton, such as distinct F-actin-rich membrane structures to surround the pathogen. The macrophage morphological changes observed on these four DAMs are key to understand the defense capability of these cells against this fungal pathogen. This work has contributed to the knowledge of the influence that the extracellular matrix and its components can exert on macrophage metabolism, immunocompetence and capacity to respond to the microenvironment in a possible infection scenario. |
format | Online Article Text |
id | pubmed-8156393 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-81563932021-05-28 Candida albicans/Macrophage Biointerface on Human and Porcine Decellularized Adipose Matrices Cicuéndez, Mónica Casarrubios, Laura Feito, María José Madarieta, Iratxe Garcia-Urkia, Nerea Murua, Olatz Olalde, Beatriz Briz, Nerea Diez-Orejas, Rosalía Portolés, María Teresa J Fungi (Basel) Article Macrophages, cells effective in sensing, internalizing and killing Candida albicans, are intertwined with the extracellular matrix (ECM) through different signals, which include the release of specific cytokines. Due to the importance of these interactions, the employment of in vitro models mimicking a fungal infection scenario is essential to evaluate the ECM effects on the macrophage response. In this work, we have analyzed the effects of human and porcine decellularized adipose matrices (DAMs), obtained by either enzymatic or organic solvent treatment, on the macrophage/Candida albicans interface. The present study has allowed us to detect differences on the activation of macrophages cultured on either human- or porcine-derived DAMs, evidencing changes in the macrophage actin cytoskeleton, such as distinct F-actin-rich membrane structures to surround the pathogen. The macrophage morphological changes observed on these four DAMs are key to understand the defense capability of these cells against this fungal pathogen. This work has contributed to the knowledge of the influence that the extracellular matrix and its components can exert on macrophage metabolism, immunocompetence and capacity to respond to the microenvironment in a possible infection scenario. MDPI 2021-05-17 /pmc/articles/PMC8156393/ /pubmed/34067785 http://dx.doi.org/10.3390/jof7050392 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cicuéndez, Mónica Casarrubios, Laura Feito, María José Madarieta, Iratxe Garcia-Urkia, Nerea Murua, Olatz Olalde, Beatriz Briz, Nerea Diez-Orejas, Rosalía Portolés, María Teresa Candida albicans/Macrophage Biointerface on Human and Porcine Decellularized Adipose Matrices |
title | Candida albicans/Macrophage Biointerface on Human and Porcine Decellularized Adipose Matrices |
title_full | Candida albicans/Macrophage Biointerface on Human and Porcine Decellularized Adipose Matrices |
title_fullStr | Candida albicans/Macrophage Biointerface on Human and Porcine Decellularized Adipose Matrices |
title_full_unstemmed | Candida albicans/Macrophage Biointerface on Human and Porcine Decellularized Adipose Matrices |
title_short | Candida albicans/Macrophage Biointerface on Human and Porcine Decellularized Adipose Matrices |
title_sort | candida albicans/macrophage biointerface on human and porcine decellularized adipose matrices |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8156393/ https://www.ncbi.nlm.nih.gov/pubmed/34067785 http://dx.doi.org/10.3390/jof7050392 |
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