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Candida albicans/Macrophage Biointerface on Human and Porcine Decellularized Adipose Matrices

Macrophages, cells effective in sensing, internalizing and killing Candida albicans, are intertwined with the extracellular matrix (ECM) through different signals, which include the release of specific cytokines. Due to the importance of these interactions, the employment of in vitro models mimickin...

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Autores principales: Cicuéndez, Mónica, Casarrubios, Laura, Feito, María José, Madarieta, Iratxe, Garcia-Urkia, Nerea, Murua, Olatz, Olalde, Beatriz, Briz, Nerea, Diez-Orejas, Rosalía, Portolés, María Teresa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8156393/
https://www.ncbi.nlm.nih.gov/pubmed/34067785
http://dx.doi.org/10.3390/jof7050392
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author Cicuéndez, Mónica
Casarrubios, Laura
Feito, María José
Madarieta, Iratxe
Garcia-Urkia, Nerea
Murua, Olatz
Olalde, Beatriz
Briz, Nerea
Diez-Orejas, Rosalía
Portolés, María Teresa
author_facet Cicuéndez, Mónica
Casarrubios, Laura
Feito, María José
Madarieta, Iratxe
Garcia-Urkia, Nerea
Murua, Olatz
Olalde, Beatriz
Briz, Nerea
Diez-Orejas, Rosalía
Portolés, María Teresa
author_sort Cicuéndez, Mónica
collection PubMed
description Macrophages, cells effective in sensing, internalizing and killing Candida albicans, are intertwined with the extracellular matrix (ECM) through different signals, which include the release of specific cytokines. Due to the importance of these interactions, the employment of in vitro models mimicking a fungal infection scenario is essential to evaluate the ECM effects on the macrophage response. In this work, we have analyzed the effects of human and porcine decellularized adipose matrices (DAMs), obtained by either enzymatic or organic solvent treatment, on the macrophage/Candida albicans interface. The present study has allowed us to detect differences on the activation of macrophages cultured on either human- or porcine-derived DAMs, evidencing changes in the macrophage actin cytoskeleton, such as distinct F-actin-rich membrane structures to surround the pathogen. The macrophage morphological changes observed on these four DAMs are key to understand the defense capability of these cells against this fungal pathogen. This work has contributed to the knowledge of the influence that the extracellular matrix and its components can exert on macrophage metabolism, immunocompetence and capacity to respond to the microenvironment in a possible infection scenario.
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spelling pubmed-81563932021-05-28 Candida albicans/Macrophage Biointerface on Human and Porcine Decellularized Adipose Matrices Cicuéndez, Mónica Casarrubios, Laura Feito, María José Madarieta, Iratxe Garcia-Urkia, Nerea Murua, Olatz Olalde, Beatriz Briz, Nerea Diez-Orejas, Rosalía Portolés, María Teresa J Fungi (Basel) Article Macrophages, cells effective in sensing, internalizing and killing Candida albicans, are intertwined with the extracellular matrix (ECM) through different signals, which include the release of specific cytokines. Due to the importance of these interactions, the employment of in vitro models mimicking a fungal infection scenario is essential to evaluate the ECM effects on the macrophage response. In this work, we have analyzed the effects of human and porcine decellularized adipose matrices (DAMs), obtained by either enzymatic or organic solvent treatment, on the macrophage/Candida albicans interface. The present study has allowed us to detect differences on the activation of macrophages cultured on either human- or porcine-derived DAMs, evidencing changes in the macrophage actin cytoskeleton, such as distinct F-actin-rich membrane structures to surround the pathogen. The macrophage morphological changes observed on these four DAMs are key to understand the defense capability of these cells against this fungal pathogen. This work has contributed to the knowledge of the influence that the extracellular matrix and its components can exert on macrophage metabolism, immunocompetence and capacity to respond to the microenvironment in a possible infection scenario. MDPI 2021-05-17 /pmc/articles/PMC8156393/ /pubmed/34067785 http://dx.doi.org/10.3390/jof7050392 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cicuéndez, Mónica
Casarrubios, Laura
Feito, María José
Madarieta, Iratxe
Garcia-Urkia, Nerea
Murua, Olatz
Olalde, Beatriz
Briz, Nerea
Diez-Orejas, Rosalía
Portolés, María Teresa
Candida albicans/Macrophage Biointerface on Human and Porcine Decellularized Adipose Matrices
title Candida albicans/Macrophage Biointerface on Human and Porcine Decellularized Adipose Matrices
title_full Candida albicans/Macrophage Biointerface on Human and Porcine Decellularized Adipose Matrices
title_fullStr Candida albicans/Macrophage Biointerface on Human and Porcine Decellularized Adipose Matrices
title_full_unstemmed Candida albicans/Macrophage Biointerface on Human and Porcine Decellularized Adipose Matrices
title_short Candida albicans/Macrophage Biointerface on Human and Porcine Decellularized Adipose Matrices
title_sort candida albicans/macrophage biointerface on human and porcine decellularized adipose matrices
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8156393/
https://www.ncbi.nlm.nih.gov/pubmed/34067785
http://dx.doi.org/10.3390/jof7050392
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