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Antimicrobial Activity of Cyclic-Monomeric and Dimeric Derivatives of the Snail-Derived Peptide Cm-p5 against Viral and Multidrug-Resistant Bacterial Strains

Cm-p5 is a snail-derived antimicrobial peptide, which demonstrated antifungal activity against the pathogenic strains of Candida albicans. Previously we synthetized a cyclic monomer as well as a parallel and an antiparallel dimer of Cm-p5 with improved antifungal activity. Considering the alarming i...

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Autores principales: González-García, Melaine, Morales-Vicente, Fidel, Pico, Erbio Díaz, Garay, Hilda, Rivera, Daniel G., Grieshober, Mark, Raluca Olari, Lia, Groß, Rüdiger, Conzelmann, Carina, Krüger, Franziska, Zech, Fabian, Prelli Bozzo, Caterina, Müller, Janis A., Zelikin, Alexander, Raber, Heinz, Kubiczek, Dennis, Rosenau, Frank, Münch, Jan, Stenger, Steffen, Spellerberg, Barbara, Franco, Octavio L., Rodriguez Alfonso, Armando A., Ständker, Ludger, Otero-Gonzalez, Anselmo J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8156702/
https://www.ncbi.nlm.nih.gov/pubmed/34067685
http://dx.doi.org/10.3390/biom11050745
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author González-García, Melaine
Morales-Vicente, Fidel
Pico, Erbio Díaz
Garay, Hilda
Rivera, Daniel G.
Grieshober, Mark
Raluca Olari, Lia
Groß, Rüdiger
Conzelmann, Carina
Krüger, Franziska
Zech, Fabian
Prelli Bozzo, Caterina
Müller, Janis A.
Zelikin, Alexander
Raber, Heinz
Kubiczek, Dennis
Rosenau, Frank
Münch, Jan
Stenger, Steffen
Spellerberg, Barbara
Franco, Octavio L.
Rodriguez Alfonso, Armando A.
Ständker, Ludger
Otero-Gonzalez, Anselmo J.
author_facet González-García, Melaine
Morales-Vicente, Fidel
Pico, Erbio Díaz
Garay, Hilda
Rivera, Daniel G.
Grieshober, Mark
Raluca Olari, Lia
Groß, Rüdiger
Conzelmann, Carina
Krüger, Franziska
Zech, Fabian
Prelli Bozzo, Caterina
Müller, Janis A.
Zelikin, Alexander
Raber, Heinz
Kubiczek, Dennis
Rosenau, Frank
Münch, Jan
Stenger, Steffen
Spellerberg, Barbara
Franco, Octavio L.
Rodriguez Alfonso, Armando A.
Ständker, Ludger
Otero-Gonzalez, Anselmo J.
author_sort González-García, Melaine
collection PubMed
description Cm-p5 is a snail-derived antimicrobial peptide, which demonstrated antifungal activity against the pathogenic strains of Candida albicans. Previously we synthetized a cyclic monomer as well as a parallel and an antiparallel dimer of Cm-p5 with improved antifungal activity. Considering the alarming increase of microbial resistance to conventional antibiotics, here we evaluated the antimicrobial activity of these derivatives against multiresistant and problematic bacteria and against important viral agents. The three peptides showed a moderate activity against Pseudomonas aeruginosa, Klebsiella pneumoniae Extended Spectrum β-Lactamase (ESBL), and Streptococcus agalactiae, with MIC values > 100 µg/mL. They exerted a considerable activity with MIC values between 25–50 µg/mL against Acinetobacter baumanii and Enterococcus faecium. In addition, the two dimers showed a moderate activity against Pseudomonas aeruginosa PA14. The three Cm-p5 derivatives inhibited a virulent extracellular strain of Mycobacterium tuberculosis, in a dose-dependent manner. Moreover, they inhibited Herpes Simplex Virus 2 (HSV-2) infection in a concentration-dependent manner, but had no effect on infection by the Zika Virus (ZIKV) or pseudoparticles of Severe Acute Respiratory Syndrome Corona Virus 2 (SARS-CoV-2). At concentrations of >100 µg/mL, the three new Cm-p5 derivatives showed toxicity on different eukaryotic cells tested. Considering a certain cell toxicity but a potential interesting activity against the multiresistant strains of bacteria and HSV-2, our compounds require future structural optimization.
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spelling pubmed-81567022021-05-28 Antimicrobial Activity of Cyclic-Monomeric and Dimeric Derivatives of the Snail-Derived Peptide Cm-p5 against Viral and Multidrug-Resistant Bacterial Strains González-García, Melaine Morales-Vicente, Fidel Pico, Erbio Díaz Garay, Hilda Rivera, Daniel G. Grieshober, Mark Raluca Olari, Lia Groß, Rüdiger Conzelmann, Carina Krüger, Franziska Zech, Fabian Prelli Bozzo, Caterina Müller, Janis A. Zelikin, Alexander Raber, Heinz Kubiczek, Dennis Rosenau, Frank Münch, Jan Stenger, Steffen Spellerberg, Barbara Franco, Octavio L. Rodriguez Alfonso, Armando A. Ständker, Ludger Otero-Gonzalez, Anselmo J. Biomolecules Article Cm-p5 is a snail-derived antimicrobial peptide, which demonstrated antifungal activity against the pathogenic strains of Candida albicans. Previously we synthetized a cyclic monomer as well as a parallel and an antiparallel dimer of Cm-p5 with improved antifungal activity. Considering the alarming increase of microbial resistance to conventional antibiotics, here we evaluated the antimicrobial activity of these derivatives against multiresistant and problematic bacteria and against important viral agents. The three peptides showed a moderate activity against Pseudomonas aeruginosa, Klebsiella pneumoniae Extended Spectrum β-Lactamase (ESBL), and Streptococcus agalactiae, with MIC values > 100 µg/mL. They exerted a considerable activity with MIC values between 25–50 µg/mL against Acinetobacter baumanii and Enterococcus faecium. In addition, the two dimers showed a moderate activity against Pseudomonas aeruginosa PA14. The three Cm-p5 derivatives inhibited a virulent extracellular strain of Mycobacterium tuberculosis, in a dose-dependent manner. Moreover, they inhibited Herpes Simplex Virus 2 (HSV-2) infection in a concentration-dependent manner, but had no effect on infection by the Zika Virus (ZIKV) or pseudoparticles of Severe Acute Respiratory Syndrome Corona Virus 2 (SARS-CoV-2). At concentrations of >100 µg/mL, the three new Cm-p5 derivatives showed toxicity on different eukaryotic cells tested. Considering a certain cell toxicity but a potential interesting activity against the multiresistant strains of bacteria and HSV-2, our compounds require future structural optimization. MDPI 2021-05-17 /pmc/articles/PMC8156702/ /pubmed/34067685 http://dx.doi.org/10.3390/biom11050745 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
González-García, Melaine
Morales-Vicente, Fidel
Pico, Erbio Díaz
Garay, Hilda
Rivera, Daniel G.
Grieshober, Mark
Raluca Olari, Lia
Groß, Rüdiger
Conzelmann, Carina
Krüger, Franziska
Zech, Fabian
Prelli Bozzo, Caterina
Müller, Janis A.
Zelikin, Alexander
Raber, Heinz
Kubiczek, Dennis
Rosenau, Frank
Münch, Jan
Stenger, Steffen
Spellerberg, Barbara
Franco, Octavio L.
Rodriguez Alfonso, Armando A.
Ständker, Ludger
Otero-Gonzalez, Anselmo J.
Antimicrobial Activity of Cyclic-Monomeric and Dimeric Derivatives of the Snail-Derived Peptide Cm-p5 against Viral and Multidrug-Resistant Bacterial Strains
title Antimicrobial Activity of Cyclic-Monomeric and Dimeric Derivatives of the Snail-Derived Peptide Cm-p5 against Viral and Multidrug-Resistant Bacterial Strains
title_full Antimicrobial Activity of Cyclic-Monomeric and Dimeric Derivatives of the Snail-Derived Peptide Cm-p5 against Viral and Multidrug-Resistant Bacterial Strains
title_fullStr Antimicrobial Activity of Cyclic-Monomeric and Dimeric Derivatives of the Snail-Derived Peptide Cm-p5 against Viral and Multidrug-Resistant Bacterial Strains
title_full_unstemmed Antimicrobial Activity of Cyclic-Monomeric and Dimeric Derivatives of the Snail-Derived Peptide Cm-p5 against Viral and Multidrug-Resistant Bacterial Strains
title_short Antimicrobial Activity of Cyclic-Monomeric and Dimeric Derivatives of the Snail-Derived Peptide Cm-p5 against Viral and Multidrug-Resistant Bacterial Strains
title_sort antimicrobial activity of cyclic-monomeric and dimeric derivatives of the snail-derived peptide cm-p5 against viral and multidrug-resistant bacterial strains
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8156702/
https://www.ncbi.nlm.nih.gov/pubmed/34067685
http://dx.doi.org/10.3390/biom11050745
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