Cargando…

Chemical Composition and Biological Properties of Two Jatropha Species: Different Parts and Different Extraction Methods

Jatropha L. species, in particular, J. curcas and J. gossypiifolia, are well known medicinal plants used for treating various diseases. In the present study, leaf and stem bark extracts of J. curcas and J. gossypiifolia obtained by maceration or homogenizer assisted extraction, were investigated for...

Descripción completa

Detalles Bibliográficos
Autores principales: Zengin, Gokhan, Mahomoodally, Mohamad Fawzi, Sinan, Kouadio Ibrahime, Ak, Gunes, Etienne, Ouattara Katinan, Sharmeen, Jugreet B., Brunetti, Luigi, Leone, Sheila, Di Simone, Simonetta Cristina, Recinella, Lucia, Chiavaroli, Annalisa, Menghini, Luigi, Orlando, Giustino, Jekő, József, Cziáky, Zoltán, Ferrante, Claudio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8156752/
https://www.ncbi.nlm.nih.gov/pubmed/34067702
http://dx.doi.org/10.3390/antiox10050792
_version_ 1783699520768442368
author Zengin, Gokhan
Mahomoodally, Mohamad Fawzi
Sinan, Kouadio Ibrahime
Ak, Gunes
Etienne, Ouattara Katinan
Sharmeen, Jugreet B.
Brunetti, Luigi
Leone, Sheila
Di Simone, Simonetta Cristina
Recinella, Lucia
Chiavaroli, Annalisa
Menghini, Luigi
Orlando, Giustino
Jekő, József
Cziáky, Zoltán
Ferrante, Claudio
author_facet Zengin, Gokhan
Mahomoodally, Mohamad Fawzi
Sinan, Kouadio Ibrahime
Ak, Gunes
Etienne, Ouattara Katinan
Sharmeen, Jugreet B.
Brunetti, Luigi
Leone, Sheila
Di Simone, Simonetta Cristina
Recinella, Lucia
Chiavaroli, Annalisa
Menghini, Luigi
Orlando, Giustino
Jekő, József
Cziáky, Zoltán
Ferrante, Claudio
author_sort Zengin, Gokhan
collection PubMed
description Jatropha L. species, in particular, J. curcas and J. gossypiifolia, are well known medicinal plants used for treating various diseases. In the present study, leaf and stem bark extracts of J. curcas and J. gossypiifolia obtained by maceration or homogenizer assisted extraction, were investigated for their phytochemical contents and biological potential as antioxidants, enzyme inhibitors and neuromodulators. In this regard, the gene expression of tumor necrosis factor α (TNFα) and brain-derived neurotrophic factor (BDNF) was investigated in hypothalamic HypoE22 cells. Finally, a bioinformatics analysis was carried out with the aim to unravel the putative mechanisms consistent with both metabolomic fingerprints and pharmacological effects. The leaf extracts of J. curcas showed higher total phenolic content (TPC) and total flavonoid content (TFC) than the stem bark extracts (range: 5.79–48.95 mg GAE/g and 1.64–13.99 mg RE/g, respectively), while J. gossypiifolia possessed TPC and TFC in the range of 42.62–62.83 mg GAE/g and 6.97–17.63 mg RE/g, respectively. HPLC-MS/MS analysis revealed that the leaf extracts of both species obtained by homogenizer assisted extraction are richer in phytochemical compounds compared to the stem bark extracts obtained by the same extraction method. In vitro antioxidant potentials were also demonstrated in different assays (DPPH: 6.89–193.93 mg TE/g, ABTS: 20.20–255.39 mg TE/g, CUPRAC: 21.07–333.30 mg TE/g, FRAP: 14.02–168.93 mg TE/g, metal chelating activity: 3.21–17.51 mg EDTAE/g and phosphomolybdenum assay: 1.76–3.55 mmol TE/g). In particular, the leaf extract of J. curcas and the stem bark extract of J. gossypiifolia, both obtained by homogenizer assisted extraction, showed the most potent antioxidant capacity in terms of free radical scavenging and reducing activity, which could be related to their higher TPC and TFC. Furthermore, anti-neurodegenerative (acetylcholinesterase inhibition: 1.12–2.36 mg GALAE/g; butyrylcholinetserase inhibition: 0.50–3.68 mg GALAE/g), anti-hyperpigmentation (tyrosinase inhibition: 38.14–57.59 mg KAE/g) and antidiabetic (amylase inhibition: 0.28–0.62 mmol ACAE/g; glucosidase inhibition: 0.65–0.81 mmol ACAE/g) properties were displayed differentially by the different extracts. Additionally, the extracts were effective in reducing the gene expression of both TNFα and BDNF, which could be partially mediated by phenolic compounds such as naringenin, apigenin and quercetin. Indeed, the scientific data obtained from the present study complement the several other reports highlighting the pharmacological potentials of these two species, thus supporting their uses as therapeutically active plants.
format Online
Article
Text
id pubmed-8156752
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-81567522021-05-28 Chemical Composition and Biological Properties of Two Jatropha Species: Different Parts and Different Extraction Methods Zengin, Gokhan Mahomoodally, Mohamad Fawzi Sinan, Kouadio Ibrahime Ak, Gunes Etienne, Ouattara Katinan Sharmeen, Jugreet B. Brunetti, Luigi Leone, Sheila Di Simone, Simonetta Cristina Recinella, Lucia Chiavaroli, Annalisa Menghini, Luigi Orlando, Giustino Jekő, József Cziáky, Zoltán Ferrante, Claudio Antioxidants (Basel) Article Jatropha L. species, in particular, J. curcas and J. gossypiifolia, are well known medicinal plants used for treating various diseases. In the present study, leaf and stem bark extracts of J. curcas and J. gossypiifolia obtained by maceration or homogenizer assisted extraction, were investigated for their phytochemical contents and biological potential as antioxidants, enzyme inhibitors and neuromodulators. In this regard, the gene expression of tumor necrosis factor α (TNFα) and brain-derived neurotrophic factor (BDNF) was investigated in hypothalamic HypoE22 cells. Finally, a bioinformatics analysis was carried out with the aim to unravel the putative mechanisms consistent with both metabolomic fingerprints and pharmacological effects. The leaf extracts of J. curcas showed higher total phenolic content (TPC) and total flavonoid content (TFC) than the stem bark extracts (range: 5.79–48.95 mg GAE/g and 1.64–13.99 mg RE/g, respectively), while J. gossypiifolia possessed TPC and TFC in the range of 42.62–62.83 mg GAE/g and 6.97–17.63 mg RE/g, respectively. HPLC-MS/MS analysis revealed that the leaf extracts of both species obtained by homogenizer assisted extraction are richer in phytochemical compounds compared to the stem bark extracts obtained by the same extraction method. In vitro antioxidant potentials were also demonstrated in different assays (DPPH: 6.89–193.93 mg TE/g, ABTS: 20.20–255.39 mg TE/g, CUPRAC: 21.07–333.30 mg TE/g, FRAP: 14.02–168.93 mg TE/g, metal chelating activity: 3.21–17.51 mg EDTAE/g and phosphomolybdenum assay: 1.76–3.55 mmol TE/g). In particular, the leaf extract of J. curcas and the stem bark extract of J. gossypiifolia, both obtained by homogenizer assisted extraction, showed the most potent antioxidant capacity in terms of free radical scavenging and reducing activity, which could be related to their higher TPC and TFC. Furthermore, anti-neurodegenerative (acetylcholinesterase inhibition: 1.12–2.36 mg GALAE/g; butyrylcholinetserase inhibition: 0.50–3.68 mg GALAE/g), anti-hyperpigmentation (tyrosinase inhibition: 38.14–57.59 mg KAE/g) and antidiabetic (amylase inhibition: 0.28–0.62 mmol ACAE/g; glucosidase inhibition: 0.65–0.81 mmol ACAE/g) properties were displayed differentially by the different extracts. Additionally, the extracts were effective in reducing the gene expression of both TNFα and BDNF, which could be partially mediated by phenolic compounds such as naringenin, apigenin and quercetin. Indeed, the scientific data obtained from the present study complement the several other reports highlighting the pharmacological potentials of these two species, thus supporting their uses as therapeutically active plants. MDPI 2021-05-17 /pmc/articles/PMC8156752/ /pubmed/34067702 http://dx.doi.org/10.3390/antiox10050792 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zengin, Gokhan
Mahomoodally, Mohamad Fawzi
Sinan, Kouadio Ibrahime
Ak, Gunes
Etienne, Ouattara Katinan
Sharmeen, Jugreet B.
Brunetti, Luigi
Leone, Sheila
Di Simone, Simonetta Cristina
Recinella, Lucia
Chiavaroli, Annalisa
Menghini, Luigi
Orlando, Giustino
Jekő, József
Cziáky, Zoltán
Ferrante, Claudio
Chemical Composition and Biological Properties of Two Jatropha Species: Different Parts and Different Extraction Methods
title Chemical Composition and Biological Properties of Two Jatropha Species: Different Parts and Different Extraction Methods
title_full Chemical Composition and Biological Properties of Two Jatropha Species: Different Parts and Different Extraction Methods
title_fullStr Chemical Composition and Biological Properties of Two Jatropha Species: Different Parts and Different Extraction Methods
title_full_unstemmed Chemical Composition and Biological Properties of Two Jatropha Species: Different Parts and Different Extraction Methods
title_short Chemical Composition and Biological Properties of Two Jatropha Species: Different Parts and Different Extraction Methods
title_sort chemical composition and biological properties of two jatropha species: different parts and different extraction methods
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8156752/
https://www.ncbi.nlm.nih.gov/pubmed/34067702
http://dx.doi.org/10.3390/antiox10050792
work_keys_str_mv AT zengingokhan chemicalcompositionandbiologicalpropertiesoftwojatrophaspeciesdifferentpartsanddifferentextractionmethods
AT mahomoodallymohamadfawzi chemicalcompositionandbiologicalpropertiesoftwojatrophaspeciesdifferentpartsanddifferentextractionmethods
AT sinankouadioibrahime chemicalcompositionandbiologicalpropertiesoftwojatrophaspeciesdifferentpartsanddifferentextractionmethods
AT akgunes chemicalcompositionandbiologicalpropertiesoftwojatrophaspeciesdifferentpartsanddifferentextractionmethods
AT etienneouattarakatinan chemicalcompositionandbiologicalpropertiesoftwojatrophaspeciesdifferentpartsanddifferentextractionmethods
AT sharmeenjugreetb chemicalcompositionandbiologicalpropertiesoftwojatrophaspeciesdifferentpartsanddifferentextractionmethods
AT brunettiluigi chemicalcompositionandbiologicalpropertiesoftwojatrophaspeciesdifferentpartsanddifferentextractionmethods
AT leonesheila chemicalcompositionandbiologicalpropertiesoftwojatrophaspeciesdifferentpartsanddifferentextractionmethods
AT disimonesimonettacristina chemicalcompositionandbiologicalpropertiesoftwojatrophaspeciesdifferentpartsanddifferentextractionmethods
AT recinellalucia chemicalcompositionandbiologicalpropertiesoftwojatrophaspeciesdifferentpartsanddifferentextractionmethods
AT chiavaroliannalisa chemicalcompositionandbiologicalpropertiesoftwojatrophaspeciesdifferentpartsanddifferentextractionmethods
AT menghiniluigi chemicalcompositionandbiologicalpropertiesoftwojatrophaspeciesdifferentpartsanddifferentextractionmethods
AT orlandogiustino chemicalcompositionandbiologicalpropertiesoftwojatrophaspeciesdifferentpartsanddifferentextractionmethods
AT jekojozsef chemicalcompositionandbiologicalpropertiesoftwojatrophaspeciesdifferentpartsanddifferentextractionmethods
AT cziakyzoltan chemicalcompositionandbiologicalpropertiesoftwojatrophaspeciesdifferentpartsanddifferentextractionmethods
AT ferranteclaudio chemicalcompositionandbiologicalpropertiesoftwojatrophaspeciesdifferentpartsanddifferentextractionmethods