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Single-Use Bioreactors for Human Pluripotent and Adult Stem Cells: Towards Regenerative Medicine Applications

Research on human stem cells, such as pluripotent stem cells and mesenchymal stromal cells, has shown much promise in their use for regenerative medicine approaches. However, their use in patients requires large-scale expansion systems while maintaining the quality of the cells. Due to their charact...

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Detalles Bibliográficos
Autores principales: Nogueira, Diogo E.S., Cabral, Joaquim M.S., Rodrigues, Carlos A.V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8156863/
https://www.ncbi.nlm.nih.gov/pubmed/34067549
http://dx.doi.org/10.3390/bioengineering8050068
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author Nogueira, Diogo E.S.
Cabral, Joaquim M.S.
Rodrigues, Carlos A.V.
author_facet Nogueira, Diogo E.S.
Cabral, Joaquim M.S.
Rodrigues, Carlos A.V.
author_sort Nogueira, Diogo E.S.
collection PubMed
description Research on human stem cells, such as pluripotent stem cells and mesenchymal stromal cells, has shown much promise in their use for regenerative medicine approaches. However, their use in patients requires large-scale expansion systems while maintaining the quality of the cells. Due to their characteristics, bioreactors have been regarded as ideal platforms to harbour stem cell biomanufacturing at a large scale. Specifically, single-use bioreactors have been recommended by regulatory agencies due to reducing the risk of product contamination, and many different systems have already been developed. This review describes single-use bioreactor platforms which have been used for human stem cell expansion and differentiation, along with their comparison with reusable systems in the development of a stem cell bioprocess for clinical applications.
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spelling pubmed-81568632021-05-28 Single-Use Bioreactors for Human Pluripotent and Adult Stem Cells: Towards Regenerative Medicine Applications Nogueira, Diogo E.S. Cabral, Joaquim M.S. Rodrigues, Carlos A.V. Bioengineering (Basel) Review Research on human stem cells, such as pluripotent stem cells and mesenchymal stromal cells, has shown much promise in their use for regenerative medicine approaches. However, their use in patients requires large-scale expansion systems while maintaining the quality of the cells. Due to their characteristics, bioreactors have been regarded as ideal platforms to harbour stem cell biomanufacturing at a large scale. Specifically, single-use bioreactors have been recommended by regulatory agencies due to reducing the risk of product contamination, and many different systems have already been developed. This review describes single-use bioreactor platforms which have been used for human stem cell expansion and differentiation, along with their comparison with reusable systems in the development of a stem cell bioprocess for clinical applications. MDPI 2021-05-17 /pmc/articles/PMC8156863/ /pubmed/34067549 http://dx.doi.org/10.3390/bioengineering8050068 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Nogueira, Diogo E.S.
Cabral, Joaquim M.S.
Rodrigues, Carlos A.V.
Single-Use Bioreactors for Human Pluripotent and Adult Stem Cells: Towards Regenerative Medicine Applications
title Single-Use Bioreactors for Human Pluripotent and Adult Stem Cells: Towards Regenerative Medicine Applications
title_full Single-Use Bioreactors for Human Pluripotent and Adult Stem Cells: Towards Regenerative Medicine Applications
title_fullStr Single-Use Bioreactors for Human Pluripotent and Adult Stem Cells: Towards Regenerative Medicine Applications
title_full_unstemmed Single-Use Bioreactors for Human Pluripotent and Adult Stem Cells: Towards Regenerative Medicine Applications
title_short Single-Use Bioreactors for Human Pluripotent and Adult Stem Cells: Towards Regenerative Medicine Applications
title_sort single-use bioreactors for human pluripotent and adult stem cells: towards regenerative medicine applications
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8156863/
https://www.ncbi.nlm.nih.gov/pubmed/34067549
http://dx.doi.org/10.3390/bioengineering8050068
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