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Single-Use Bioreactors for Human Pluripotent and Adult Stem Cells: Towards Regenerative Medicine Applications
Research on human stem cells, such as pluripotent stem cells and mesenchymal stromal cells, has shown much promise in their use for regenerative medicine approaches. However, their use in patients requires large-scale expansion systems while maintaining the quality of the cells. Due to their charact...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8156863/ https://www.ncbi.nlm.nih.gov/pubmed/34067549 http://dx.doi.org/10.3390/bioengineering8050068 |
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author | Nogueira, Diogo E.S. Cabral, Joaquim M.S. Rodrigues, Carlos A.V. |
author_facet | Nogueira, Diogo E.S. Cabral, Joaquim M.S. Rodrigues, Carlos A.V. |
author_sort | Nogueira, Diogo E.S. |
collection | PubMed |
description | Research on human stem cells, such as pluripotent stem cells and mesenchymal stromal cells, has shown much promise in their use for regenerative medicine approaches. However, their use in patients requires large-scale expansion systems while maintaining the quality of the cells. Due to their characteristics, bioreactors have been regarded as ideal platforms to harbour stem cell biomanufacturing at a large scale. Specifically, single-use bioreactors have been recommended by regulatory agencies due to reducing the risk of product contamination, and many different systems have already been developed. This review describes single-use bioreactor platforms which have been used for human stem cell expansion and differentiation, along with their comparison with reusable systems in the development of a stem cell bioprocess for clinical applications. |
format | Online Article Text |
id | pubmed-8156863 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-81568632021-05-28 Single-Use Bioreactors for Human Pluripotent and Adult Stem Cells: Towards Regenerative Medicine Applications Nogueira, Diogo E.S. Cabral, Joaquim M.S. Rodrigues, Carlos A.V. Bioengineering (Basel) Review Research on human stem cells, such as pluripotent stem cells and mesenchymal stromal cells, has shown much promise in their use for regenerative medicine approaches. However, their use in patients requires large-scale expansion systems while maintaining the quality of the cells. Due to their characteristics, bioreactors have been regarded as ideal platforms to harbour stem cell biomanufacturing at a large scale. Specifically, single-use bioreactors have been recommended by regulatory agencies due to reducing the risk of product contamination, and many different systems have already been developed. This review describes single-use bioreactor platforms which have been used for human stem cell expansion and differentiation, along with their comparison with reusable systems in the development of a stem cell bioprocess for clinical applications. MDPI 2021-05-17 /pmc/articles/PMC8156863/ /pubmed/34067549 http://dx.doi.org/10.3390/bioengineering8050068 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Nogueira, Diogo E.S. Cabral, Joaquim M.S. Rodrigues, Carlos A.V. Single-Use Bioreactors for Human Pluripotent and Adult Stem Cells: Towards Regenerative Medicine Applications |
title | Single-Use Bioreactors for Human Pluripotent and Adult Stem Cells: Towards Regenerative Medicine Applications |
title_full | Single-Use Bioreactors for Human Pluripotent and Adult Stem Cells: Towards Regenerative Medicine Applications |
title_fullStr | Single-Use Bioreactors for Human Pluripotent and Adult Stem Cells: Towards Regenerative Medicine Applications |
title_full_unstemmed | Single-Use Bioreactors for Human Pluripotent and Adult Stem Cells: Towards Regenerative Medicine Applications |
title_short | Single-Use Bioreactors for Human Pluripotent and Adult Stem Cells: Towards Regenerative Medicine Applications |
title_sort | single-use bioreactors for human pluripotent and adult stem cells: towards regenerative medicine applications |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8156863/ https://www.ncbi.nlm.nih.gov/pubmed/34067549 http://dx.doi.org/10.3390/bioengineering8050068 |
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