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Effects of Three-Month Administration of High-Saturated Fat Diet and High-Polyunsaturated Fat Diets with Different Linoleic Acid (LA, C18:2n–6) to α-Linolenic Acid (ALA, C18:3n–3) Ratio on the Mouse Liver Proteome

The aim of the study was to evaluate the effect of different types of high-fat diets (HFDs) on the proteomic profile of mouse liver. The analysis included four dietary groups of mice fed a standard diet (STD group), a high-fat diet rich in SFAs (SFA group), and high-fat diets dominated by PUFAs with...

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Autores principales: Liput, Kamila P., Lepczyński, Adam, Nawrocka, Agata, Poławska, Ewa, Ogłuszka, Magdalena, Jończy, Aneta, Grzybek, Weronika, Liput, Michał, Szostak, Agnieszka, Urbański, Paweł, Roszczyk, Agnieszka, Pareek, Chandra S., Pierzchała, Mariusz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8156955/
https://www.ncbi.nlm.nih.gov/pubmed/34063343
http://dx.doi.org/10.3390/nu13051678
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author Liput, Kamila P.
Lepczyński, Adam
Nawrocka, Agata
Poławska, Ewa
Ogłuszka, Magdalena
Jończy, Aneta
Grzybek, Weronika
Liput, Michał
Szostak, Agnieszka
Urbański, Paweł
Roszczyk, Agnieszka
Pareek, Chandra S.
Pierzchała, Mariusz
author_facet Liput, Kamila P.
Lepczyński, Adam
Nawrocka, Agata
Poławska, Ewa
Ogłuszka, Magdalena
Jończy, Aneta
Grzybek, Weronika
Liput, Michał
Szostak, Agnieszka
Urbański, Paweł
Roszczyk, Agnieszka
Pareek, Chandra S.
Pierzchała, Mariusz
author_sort Liput, Kamila P.
collection PubMed
description The aim of the study was to evaluate the effect of different types of high-fat diets (HFDs) on the proteomic profile of mouse liver. The analysis included four dietary groups of mice fed a standard diet (STD group), a high-fat diet rich in SFAs (SFA group), and high-fat diets dominated by PUFAs with linoleic acid (LA, C18:2n–6) to α-linolenic acid (ALA, C18:3n–3) ratios of 14:1 (14:1 group) and 5:1 (5:1 group). After three months of diets, liver proteins were resolved by two-dimensional gel electrophoresis (2DE) using 17 cm non-linear 3–10 pH gradient strips. Protein spots with different expression were identified by MALDI-TOF/TOF. The expression of 13 liver proteins was changed in the SFA group compared to the STD group (↓: ALB, APOA1, IVD, MAT1A, OAT and PHB; ↑: ALDH1L1, UniProtKB—Q91V76, GALK1, GPD1, HMGCS2, KHK and TKFC). Eleven proteins with altered expression were recorded in the 14:1 group compared to the SFA group (↓: ARG1, FTL1, GPD1, HGD, HMGCS2 and MAT1A; ↑: APOA1, CA3, GLO1, HDHD3 and IVD). The expression of 11 proteins was altered in the 5:1 group compared to the SFA group (↓: ATP5F1B, FTL1, GALK1, HGD, HSPA9, HSPD1, PC and TKFC; ↑: ACAT2, CA3 and GSTP1). High-PUFA diets significantly affected the expression of proteins involved in, e.g., carbohydrate metabolism, and had varying effects on plasma total cholesterol and glucose levels. The outcomes of this study revealed crucial liver proteins affected by different high-fat diets.
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spelling pubmed-81569552021-05-28 Effects of Three-Month Administration of High-Saturated Fat Diet and High-Polyunsaturated Fat Diets with Different Linoleic Acid (LA, C18:2n–6) to α-Linolenic Acid (ALA, C18:3n–3) Ratio on the Mouse Liver Proteome Liput, Kamila P. Lepczyński, Adam Nawrocka, Agata Poławska, Ewa Ogłuszka, Magdalena Jończy, Aneta Grzybek, Weronika Liput, Michał Szostak, Agnieszka Urbański, Paweł Roszczyk, Agnieszka Pareek, Chandra S. Pierzchała, Mariusz Nutrients Article The aim of the study was to evaluate the effect of different types of high-fat diets (HFDs) on the proteomic profile of mouse liver. The analysis included four dietary groups of mice fed a standard diet (STD group), a high-fat diet rich in SFAs (SFA group), and high-fat diets dominated by PUFAs with linoleic acid (LA, C18:2n–6) to α-linolenic acid (ALA, C18:3n–3) ratios of 14:1 (14:1 group) and 5:1 (5:1 group). After three months of diets, liver proteins were resolved by two-dimensional gel electrophoresis (2DE) using 17 cm non-linear 3–10 pH gradient strips. Protein spots with different expression were identified by MALDI-TOF/TOF. The expression of 13 liver proteins was changed in the SFA group compared to the STD group (↓: ALB, APOA1, IVD, MAT1A, OAT and PHB; ↑: ALDH1L1, UniProtKB—Q91V76, GALK1, GPD1, HMGCS2, KHK and TKFC). Eleven proteins with altered expression were recorded in the 14:1 group compared to the SFA group (↓: ARG1, FTL1, GPD1, HGD, HMGCS2 and MAT1A; ↑: APOA1, CA3, GLO1, HDHD3 and IVD). The expression of 11 proteins was altered in the 5:1 group compared to the SFA group (↓: ATP5F1B, FTL1, GALK1, HGD, HSPA9, HSPD1, PC and TKFC; ↑: ACAT2, CA3 and GSTP1). High-PUFA diets significantly affected the expression of proteins involved in, e.g., carbohydrate metabolism, and had varying effects on plasma total cholesterol and glucose levels. The outcomes of this study revealed crucial liver proteins affected by different high-fat diets. MDPI 2021-05-15 /pmc/articles/PMC8156955/ /pubmed/34063343 http://dx.doi.org/10.3390/nu13051678 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liput, Kamila P.
Lepczyński, Adam
Nawrocka, Agata
Poławska, Ewa
Ogłuszka, Magdalena
Jończy, Aneta
Grzybek, Weronika
Liput, Michał
Szostak, Agnieszka
Urbański, Paweł
Roszczyk, Agnieszka
Pareek, Chandra S.
Pierzchała, Mariusz
Effects of Three-Month Administration of High-Saturated Fat Diet and High-Polyunsaturated Fat Diets with Different Linoleic Acid (LA, C18:2n–6) to α-Linolenic Acid (ALA, C18:3n–3) Ratio on the Mouse Liver Proteome
title Effects of Three-Month Administration of High-Saturated Fat Diet and High-Polyunsaturated Fat Diets with Different Linoleic Acid (LA, C18:2n–6) to α-Linolenic Acid (ALA, C18:3n–3) Ratio on the Mouse Liver Proteome
title_full Effects of Three-Month Administration of High-Saturated Fat Diet and High-Polyunsaturated Fat Diets with Different Linoleic Acid (LA, C18:2n–6) to α-Linolenic Acid (ALA, C18:3n–3) Ratio on the Mouse Liver Proteome
title_fullStr Effects of Three-Month Administration of High-Saturated Fat Diet and High-Polyunsaturated Fat Diets with Different Linoleic Acid (LA, C18:2n–6) to α-Linolenic Acid (ALA, C18:3n–3) Ratio on the Mouse Liver Proteome
title_full_unstemmed Effects of Three-Month Administration of High-Saturated Fat Diet and High-Polyunsaturated Fat Diets with Different Linoleic Acid (LA, C18:2n–6) to α-Linolenic Acid (ALA, C18:3n–3) Ratio on the Mouse Liver Proteome
title_short Effects of Three-Month Administration of High-Saturated Fat Diet and High-Polyunsaturated Fat Diets with Different Linoleic Acid (LA, C18:2n–6) to α-Linolenic Acid (ALA, C18:3n–3) Ratio on the Mouse Liver Proteome
title_sort effects of three-month administration of high-saturated fat diet and high-polyunsaturated fat diets with different linoleic acid (la, c18:2n–6) to α-linolenic acid (ala, c18:3n–3) ratio on the mouse liver proteome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8156955/
https://www.ncbi.nlm.nih.gov/pubmed/34063343
http://dx.doi.org/10.3390/nu13051678
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