Susceptibility of South Texas Aedes aegypti to Pyriproxyfen

SIMPLE SUMMARY: We evaluated the susceptibility of an Ae. aegypti strain from the Lower Rio Grande Valley (LRGV) of South Texas to the insect growth regulator pyriproxyfen. We observed a difference in the inhibition of emergence to the lowest doses of pyriproxyfen tested between our field strain and a susceptible strain. However, the doses used are 10 times lower from the recommended application of <50 ppb for vector control programs. Our results suggest that pyriproxyfen should be an effective active ingredient in the LRGV to help reduce Ae. aegypti populations in the LRGV. ABSTRACT: An integral part to integrated mosquito management is to ensure chemical products used for area-wide control are effective against a susceptible population of mosquitoes. Prior to conducting an intervention trial using an insect growth regulator, pyriproxyfen, in South Texas to control Aedes aegypti, we conducted a larval bioassay to evaluate baseline levels of susceptibility. We used seven serially-diluted doses ranging from 2.5 ppb to 6.3 × 10(−4) ppb. We observed 100% inhibition emergence (IE) at even the lowest dose of 6.3 × 10(−4) ppb in our susceptible reference colony of Ae. aegypti Liverpool. In our field strain of Ae. aegypti (F5 colonized from South Texas) we observed 79.8% IE at 6.3 × 10(−4) ppb, 17.7% IE at 1.25 × 10(−3) ppb, 98.7% IE at 1.25 × 10(−2) ppb, and 100% emergence inhibition for the remainder of the doses. Given that commercial pyriproxyfen products are labeled for doses ranging to 50 ppb, we conclude that the field population sampled by this study are susceptible to this insect growth regulator.