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Mitigating Antibiotic Resistance Genes in Wastewater by Sequential Treatment with Novel Nanomaterials

Wastewater (WW) has been widely recognized as the major sink of a variety of emerging pathogens (EPs), antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs), which may disseminate and impact wider environments. Improving and maximizing WW treatment efficiency to remove these mic...

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Autores principales: Paruch, Lisa, Paruch, Adam M., Iordache, Tanta-Verona, Olaru, Andreea G., Sarbu, Andrei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8157218/
https://www.ncbi.nlm.nih.gov/pubmed/34063382
http://dx.doi.org/10.3390/polym13101593
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author Paruch, Lisa
Paruch, Adam M.
Iordache, Tanta-Verona
Olaru, Andreea G.
Sarbu, Andrei
author_facet Paruch, Lisa
Paruch, Adam M.
Iordache, Tanta-Verona
Olaru, Andreea G.
Sarbu, Andrei
author_sort Paruch, Lisa
collection PubMed
description Wastewater (WW) has been widely recognized as the major sink of a variety of emerging pathogens (EPs), antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs), which may disseminate and impact wider environments. Improving and maximizing WW treatment efficiency to remove these microbial hazards is fundamentally imperative. Despite a variety of physical, biological and chemical treatment technologies, the efficiency of ARG removal is still far from satisfactory. Within our recently accomplished M-ERA.NET project, novel functionalized nanomaterials, i.e., molecularly imprinted polymer (MIP) films and quaternary ammonium salt (QAS) modified kaolin microparticles, were developed and demonstrated to have significant EP removal effectiveness on both Gram-positive bacteria (GPB) and Gram-negative bacteria (GNB) from WW. As a continuation of this project, we took the further step of exploring their ARG mitigation potential. Strikingly, by applying MIP and QAS functionalized kaolin microparticles in tandem, the ARGs prevalent in wastewater treatment plants (WWTPs), e.g., blaCTXM, ermB and qnrS, can be drastically reduced by 2.7, 3.9 and 4.9 log (copies/100 mL), respectively, whereas sul1, tetO and mecA can be eliminated below their detection limits. In terms of class I integron-integrase I (intI1), a mobile genetic element (MGE) for horizontal gene transfer (HGT), 4.3 log (copies/100 mL) reduction was achieved. Overall, the novel nanomaterials exhibit outstanding performance on attenuating ARGs in WW, being superior to their control references. This finding provides additional merit to the application of developed nanomaterials for WW purification towards ARG elimination, in addition to the proven bactericidal effect.
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spelling pubmed-81572182021-05-28 Mitigating Antibiotic Resistance Genes in Wastewater by Sequential Treatment with Novel Nanomaterials Paruch, Lisa Paruch, Adam M. Iordache, Tanta-Verona Olaru, Andreea G. Sarbu, Andrei Polymers (Basel) Communication Wastewater (WW) has been widely recognized as the major sink of a variety of emerging pathogens (EPs), antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs), which may disseminate and impact wider environments. Improving and maximizing WW treatment efficiency to remove these microbial hazards is fundamentally imperative. Despite a variety of physical, biological and chemical treatment technologies, the efficiency of ARG removal is still far from satisfactory. Within our recently accomplished M-ERA.NET project, novel functionalized nanomaterials, i.e., molecularly imprinted polymer (MIP) films and quaternary ammonium salt (QAS) modified kaolin microparticles, were developed and demonstrated to have significant EP removal effectiveness on both Gram-positive bacteria (GPB) and Gram-negative bacteria (GNB) from WW. As a continuation of this project, we took the further step of exploring their ARG mitigation potential. Strikingly, by applying MIP and QAS functionalized kaolin microparticles in tandem, the ARGs prevalent in wastewater treatment plants (WWTPs), e.g., blaCTXM, ermB and qnrS, can be drastically reduced by 2.7, 3.9 and 4.9 log (copies/100 mL), respectively, whereas sul1, tetO and mecA can be eliminated below their detection limits. In terms of class I integron-integrase I (intI1), a mobile genetic element (MGE) for horizontal gene transfer (HGT), 4.3 log (copies/100 mL) reduction was achieved. Overall, the novel nanomaterials exhibit outstanding performance on attenuating ARGs in WW, being superior to their control references. This finding provides additional merit to the application of developed nanomaterials for WW purification towards ARG elimination, in addition to the proven bactericidal effect. MDPI 2021-05-15 /pmc/articles/PMC8157218/ /pubmed/34063382 http://dx.doi.org/10.3390/polym13101593 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Paruch, Lisa
Paruch, Adam M.
Iordache, Tanta-Verona
Olaru, Andreea G.
Sarbu, Andrei
Mitigating Antibiotic Resistance Genes in Wastewater by Sequential Treatment with Novel Nanomaterials
title Mitigating Antibiotic Resistance Genes in Wastewater by Sequential Treatment with Novel Nanomaterials
title_full Mitigating Antibiotic Resistance Genes in Wastewater by Sequential Treatment with Novel Nanomaterials
title_fullStr Mitigating Antibiotic Resistance Genes in Wastewater by Sequential Treatment with Novel Nanomaterials
title_full_unstemmed Mitigating Antibiotic Resistance Genes in Wastewater by Sequential Treatment with Novel Nanomaterials
title_short Mitigating Antibiotic Resistance Genes in Wastewater by Sequential Treatment with Novel Nanomaterials
title_sort mitigating antibiotic resistance genes in wastewater by sequential treatment with novel nanomaterials
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8157218/
https://www.ncbi.nlm.nih.gov/pubmed/34063382
http://dx.doi.org/10.3390/polym13101593
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