Pre-digest of unprotected DNA by Benzonase improves the representation of living skin bacteria and efficiently depletes host DNA

BACKGROUND: The identification of microbiota based on next-generation sequencing (NGS) of extracted DNA has drastically improved our understanding of the role of microbial communities in health and disease. However, DNA-based microbiome analysis cannot per se differentiate between living and dead mi...

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Detalles Bibliográficos
Autores principales: Amar, Yacine, Lagkouvardos, Ilias, Silva, Rafaela L., Ishola, Oluwaseun Ayodeji, Foesel, Bärbel U., Kublik, Susanne, Schöler, Anne, Niedermeier, Sebastian, Bleuel, Rachela, Zink, Alexander, Neuhaus, Klaus, Schloter, Michael, Biedermann, Tilo, Köberle, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8157445/
https://www.ncbi.nlm.nih.gov/pubmed/34039428
http://dx.doi.org/10.1186/s40168-021-01067-0

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