Cargando…
Dynamic contrast with reversibly photoswitchable fluorescent labels for imaging living cells
Interrogating living cells requires sensitive imaging of a large number of components in real time. The state-of-the-art of multiplexed imaging is usually limited to a few components. This review reports on the promise and the challenges of dynamic contrast to overcome this limitation.
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8157520/ https://www.ncbi.nlm.nih.gov/pubmed/34122788 http://dx.doi.org/10.1039/d0sc00182a |
_version_ | 1783699699469910016 |
---|---|
author | Chouket, Raja Pellissier-Tanon, Agnès Lemarchand, Annie Espagne, Agathe Le Saux, Thomas Jullien, Ludovic |
author_facet | Chouket, Raja Pellissier-Tanon, Agnès Lemarchand, Annie Espagne, Agathe Le Saux, Thomas Jullien, Ludovic |
author_sort | Chouket, Raja |
collection | PubMed |
description | Interrogating living cells requires sensitive imaging of a large number of components in real time. The state-of-the-art of multiplexed imaging is usually limited to a few components. This review reports on the promise and the challenges of dynamic contrast to overcome this limitation. |
format | Online Article Text |
id | pubmed-8157520 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-81575202021-06-11 Dynamic contrast with reversibly photoswitchable fluorescent labels for imaging living cells Chouket, Raja Pellissier-Tanon, Agnès Lemarchand, Annie Espagne, Agathe Le Saux, Thomas Jullien, Ludovic Chem Sci Chemistry Interrogating living cells requires sensitive imaging of a large number of components in real time. The state-of-the-art of multiplexed imaging is usually limited to a few components. This review reports on the promise and the challenges of dynamic contrast to overcome this limitation. The Royal Society of Chemistry 2020-02-25 /pmc/articles/PMC8157520/ /pubmed/34122788 http://dx.doi.org/10.1039/d0sc00182a Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Chouket, Raja Pellissier-Tanon, Agnès Lemarchand, Annie Espagne, Agathe Le Saux, Thomas Jullien, Ludovic Dynamic contrast with reversibly photoswitchable fluorescent labels for imaging living cells |
title | Dynamic contrast with reversibly photoswitchable fluorescent labels for imaging living cells |
title_full | Dynamic contrast with reversibly photoswitchable fluorescent labels for imaging living cells |
title_fullStr | Dynamic contrast with reversibly photoswitchable fluorescent labels for imaging living cells |
title_full_unstemmed | Dynamic contrast with reversibly photoswitchable fluorescent labels for imaging living cells |
title_short | Dynamic contrast with reversibly photoswitchable fluorescent labels for imaging living cells |
title_sort | dynamic contrast with reversibly photoswitchable fluorescent labels for imaging living cells |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8157520/ https://www.ncbi.nlm.nih.gov/pubmed/34122788 http://dx.doi.org/10.1039/d0sc00182a |
work_keys_str_mv | AT chouketraja dynamiccontrastwithreversiblyphotoswitchablefluorescentlabelsforimaginglivingcells AT pellissiertanonagnes dynamiccontrastwithreversiblyphotoswitchablefluorescentlabelsforimaginglivingcells AT lemarchandannie dynamiccontrastwithreversiblyphotoswitchablefluorescentlabelsforimaginglivingcells AT espagneagathe dynamiccontrastwithreversiblyphotoswitchablefluorescentlabelsforimaginglivingcells AT lesauxthomas dynamiccontrastwithreversiblyphotoswitchablefluorescentlabelsforimaginglivingcells AT jullienludovic dynamiccontrastwithreversiblyphotoswitchablefluorescentlabelsforimaginglivingcells |