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Optogenetic Approaches for the Spatiotemporal Control of Signal Transduction Pathways
Biological signals are sensed by their respective receptors and are transduced and processed by a sophisticated intracellular signaling network leading to a signal-specific cellular response. Thereby, the response to the signal depends on the strength, the frequency, and the duration of the stimulus...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8157557/ https://www.ncbi.nlm.nih.gov/pubmed/34069904 http://dx.doi.org/10.3390/ijms22105300 |
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author | Kramer, Markus M. Lataster, Levin Weber, Wilfried Radziwill, Gerald |
author_facet | Kramer, Markus M. Lataster, Levin Weber, Wilfried Radziwill, Gerald |
author_sort | Kramer, Markus M. |
collection | PubMed |
description | Biological signals are sensed by their respective receptors and are transduced and processed by a sophisticated intracellular signaling network leading to a signal-specific cellular response. Thereby, the response to the signal depends on the strength, the frequency, and the duration of the stimulus as well as on the subcellular signal progression. Optogenetic tools are based on genetically encoded light-sensing proteins facilitating the precise spatiotemporal control of signal transduction pathways and cell fate decisions in the absence of natural ligands. In this review, we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins. We briefly discuss the most common optogenetic switches and their mode of action. The main part deals with the engineering and application of optogenetic tools for the control of transmembrane receptors including receptor tyrosine kinases, the T cell receptor and integrins, and their effector proteins. We also address the hallmarks of optogenetics, the spatial and temporal control of signaling events. |
format | Online Article Text |
id | pubmed-8157557 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-81575572021-05-28 Optogenetic Approaches for the Spatiotemporal Control of Signal Transduction Pathways Kramer, Markus M. Lataster, Levin Weber, Wilfried Radziwill, Gerald Int J Mol Sci Review Biological signals are sensed by their respective receptors and are transduced and processed by a sophisticated intracellular signaling network leading to a signal-specific cellular response. Thereby, the response to the signal depends on the strength, the frequency, and the duration of the stimulus as well as on the subcellular signal progression. Optogenetic tools are based on genetically encoded light-sensing proteins facilitating the precise spatiotemporal control of signal transduction pathways and cell fate decisions in the absence of natural ligands. In this review, we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins. We briefly discuss the most common optogenetic switches and their mode of action. The main part deals with the engineering and application of optogenetic tools for the control of transmembrane receptors including receptor tyrosine kinases, the T cell receptor and integrins, and their effector proteins. We also address the hallmarks of optogenetics, the spatial and temporal control of signaling events. MDPI 2021-05-18 /pmc/articles/PMC8157557/ /pubmed/34069904 http://dx.doi.org/10.3390/ijms22105300 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Kramer, Markus M. Lataster, Levin Weber, Wilfried Radziwill, Gerald Optogenetic Approaches for the Spatiotemporal Control of Signal Transduction Pathways |
title | Optogenetic Approaches for the Spatiotemporal Control of Signal Transduction Pathways |
title_full | Optogenetic Approaches for the Spatiotemporal Control of Signal Transduction Pathways |
title_fullStr | Optogenetic Approaches for the Spatiotemporal Control of Signal Transduction Pathways |
title_full_unstemmed | Optogenetic Approaches for the Spatiotemporal Control of Signal Transduction Pathways |
title_short | Optogenetic Approaches for the Spatiotemporal Control of Signal Transduction Pathways |
title_sort | optogenetic approaches for the spatiotemporal control of signal transduction pathways |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8157557/ https://www.ncbi.nlm.nih.gov/pubmed/34069904 http://dx.doi.org/10.3390/ijms22105300 |
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