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A Specific Tubular ApoA-I Distribution Is Associated to FSGS Recurrence after Kidney Transplantation

A major complication of primary focal segmental glomerulosclerosis (FSGS) is its recurrence after kidney transplantation that happens in 30 to 40% of the patients. The diagnosis of these relapses is not always easy as the histological lesions are not highly specific and appear after the proteinuria...

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Autores principales: Jacobs-Cachá, Conxita, Puig-Gay, Natàlia, Vergara, Ander, Gabaldon, Maria-Alejandra, Sellarés, Joana, Villena-Ortiz, Yolanda, Agraz, Irene, Moreso, Francesc, Soler, Maria José, Serón, Daniel, López-Hellín, Joan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8157584/
https://www.ncbi.nlm.nih.gov/pubmed/34069888
http://dx.doi.org/10.3390/jcm10102174
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author Jacobs-Cachá, Conxita
Puig-Gay, Natàlia
Vergara, Ander
Gabaldon, Maria-Alejandra
Sellarés, Joana
Villena-Ortiz, Yolanda
Agraz, Irene
Moreso, Francesc
Soler, Maria José
Serón, Daniel
López-Hellín, Joan
author_facet Jacobs-Cachá, Conxita
Puig-Gay, Natàlia
Vergara, Ander
Gabaldon, Maria-Alejandra
Sellarés, Joana
Villena-Ortiz, Yolanda
Agraz, Irene
Moreso, Francesc
Soler, Maria José
Serón, Daniel
López-Hellín, Joan
author_sort Jacobs-Cachá, Conxita
collection PubMed
description A major complication of primary focal segmental glomerulosclerosis (FSGS) is its recurrence after kidney transplantation that happens in 30 to 40% of the patients. The diagnosis of these relapses is not always easy as the histological lesions are not highly specific and appear after the proteinuria increase. Currently, there are no accurate biomarkers to detect FSGS recurrence. Our group identified a modified form of Apolipoprotein A-I (ApoA-I), named ApoA-Ib, specifically present in the urine of recurrent FSGS patients after kidney transplantation. Aberrant forms of ApoA-I have also been described in the urine of native primary FSGS patients; this feature has been associated with prominent staining of ApoA-I at the apical membrane of the tubular cells. In this study, we aim to analyze the ApoA-I distribution in kidney allograft biopsies of recurrent FSGS patients. We detected ApoA-I by immunohistochemistry in kidney allograft biopsies of patients with FSGS relapse after kidney transplantation and in kidney allograft biopsies of patients with a disease different from FSGS in the native kidney (non-FSGS). In recurrent FSGS patients, ApoA-I was prominently localized at the brush border of the tubular cells, while in the non-FSGS patients, ApoA-I was found along the cytoplasm of the tubular cells. The localization of ApoA-I at the brush border of the tubular cells is a specific feature of primary FSGS in relapse. This suggests that ApoA-I staining in kidney biopsies, coupled with ApoA-Ib measurement in urine, could be used as a diagnostic tool of primary FSGS relapse after kidney transplantation due to its highly specific tubular distribution.
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spelling pubmed-81575842021-05-28 A Specific Tubular ApoA-I Distribution Is Associated to FSGS Recurrence after Kidney Transplantation Jacobs-Cachá, Conxita Puig-Gay, Natàlia Vergara, Ander Gabaldon, Maria-Alejandra Sellarés, Joana Villena-Ortiz, Yolanda Agraz, Irene Moreso, Francesc Soler, Maria José Serón, Daniel López-Hellín, Joan J Clin Med Article A major complication of primary focal segmental glomerulosclerosis (FSGS) is its recurrence after kidney transplantation that happens in 30 to 40% of the patients. The diagnosis of these relapses is not always easy as the histological lesions are not highly specific and appear after the proteinuria increase. Currently, there are no accurate biomarkers to detect FSGS recurrence. Our group identified a modified form of Apolipoprotein A-I (ApoA-I), named ApoA-Ib, specifically present in the urine of recurrent FSGS patients after kidney transplantation. Aberrant forms of ApoA-I have also been described in the urine of native primary FSGS patients; this feature has been associated with prominent staining of ApoA-I at the apical membrane of the tubular cells. In this study, we aim to analyze the ApoA-I distribution in kidney allograft biopsies of recurrent FSGS patients. We detected ApoA-I by immunohistochemistry in kidney allograft biopsies of patients with FSGS relapse after kidney transplantation and in kidney allograft biopsies of patients with a disease different from FSGS in the native kidney (non-FSGS). In recurrent FSGS patients, ApoA-I was prominently localized at the brush border of the tubular cells, while in the non-FSGS patients, ApoA-I was found along the cytoplasm of the tubular cells. The localization of ApoA-I at the brush border of the tubular cells is a specific feature of primary FSGS in relapse. This suggests that ApoA-I staining in kidney biopsies, coupled with ApoA-Ib measurement in urine, could be used as a diagnostic tool of primary FSGS relapse after kidney transplantation due to its highly specific tubular distribution. MDPI 2021-05-18 /pmc/articles/PMC8157584/ /pubmed/34069888 http://dx.doi.org/10.3390/jcm10102174 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jacobs-Cachá, Conxita
Puig-Gay, Natàlia
Vergara, Ander
Gabaldon, Maria-Alejandra
Sellarés, Joana
Villena-Ortiz, Yolanda
Agraz, Irene
Moreso, Francesc
Soler, Maria José
Serón, Daniel
López-Hellín, Joan
A Specific Tubular ApoA-I Distribution Is Associated to FSGS Recurrence after Kidney Transplantation
title A Specific Tubular ApoA-I Distribution Is Associated to FSGS Recurrence after Kidney Transplantation
title_full A Specific Tubular ApoA-I Distribution Is Associated to FSGS Recurrence after Kidney Transplantation
title_fullStr A Specific Tubular ApoA-I Distribution Is Associated to FSGS Recurrence after Kidney Transplantation
title_full_unstemmed A Specific Tubular ApoA-I Distribution Is Associated to FSGS Recurrence after Kidney Transplantation
title_short A Specific Tubular ApoA-I Distribution Is Associated to FSGS Recurrence after Kidney Transplantation
title_sort specific tubular apoa-i distribution is associated to fsgs recurrence after kidney transplantation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8157584/
https://www.ncbi.nlm.nih.gov/pubmed/34069888
http://dx.doi.org/10.3390/jcm10102174
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