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Effect of exercise training on skeletal muscle protein expression in relation to insulin sensitivity: Per‐protocol analysis of a randomized controlled trial (GO‐ACTIWE)

Exercise training improves peripheral insulin sensitivity and leads to molecular adaptations in the skeletal muscle. We investigated changes in the expression of key muscle proteins in the glucose metabolic pathway following active commuting by bike or leisure‐time exercise at two different intensit...

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Autores principales: Bruhn, Lea, Kjøbsted, Rasmus, Quist, Jonas Salling, Gram, Anne Sofie, Rosenkilde, Mads, Færch, Kristine, Wojtaszewski, Jørgen F.P., Stallknecht, Bente, Blond, Martin Bæk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8157763/
https://www.ncbi.nlm.nih.gov/pubmed/34042297
http://dx.doi.org/10.14814/phy2.14850
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author Bruhn, Lea
Kjøbsted, Rasmus
Quist, Jonas Salling
Gram, Anne Sofie
Rosenkilde, Mads
Færch, Kristine
Wojtaszewski, Jørgen F.P.
Stallknecht, Bente
Blond, Martin Bæk
author_facet Bruhn, Lea
Kjøbsted, Rasmus
Quist, Jonas Salling
Gram, Anne Sofie
Rosenkilde, Mads
Færch, Kristine
Wojtaszewski, Jørgen F.P.
Stallknecht, Bente
Blond, Martin Bæk
author_sort Bruhn, Lea
collection PubMed
description Exercise training improves peripheral insulin sensitivity and leads to molecular adaptations in the skeletal muscle. We investigated changes in the expression of key muscle proteins in the glucose metabolic pathway following active commuting by bike or leisure‐time exercise at two different intensities. In addition, potential associations between insulin sensitivity and muscle protein expression were examined. This per‐protocol analysis included 72 out of 130 physically inactive, healthy women and men (20–45 years) with overweight/obesity (BMI: 25–35 kg/m(2)) who completed 6 months of no intervention (CON, n = 12), active commuting by bike (BIKE, n = 14), or leisure‐time exercise of moderate (MOD, n = 28) or vigorous (VIG, n = 18) intensity. Exercise was prescribed 5 days/week with a weekly exercise energy expenditure of 1,600 kcal for women and 2,100 kcal for men. Insulin sensitivity was determined by a hyperinsulinemic euglycemic clamp and skeletal muscle biopsies were obtained from m. vastus lateralis and analyzed for protein expression at baseline and after 3 and 6 months of intervention. We found an increased expression of pyruvate dehydrogenase (PDH) in the exercise groups compared with the control group following 6 months of training. No differential effects were observed on the protein expression following moderate versus vigorous intensity exercise. In addition, we found a positive association between insulin sensitivity and the expression of glucose transporter type 4 as well as PDH. The positive association and the increase in expression of PDH after exercise training points toward a role for PDH in the training‐induced enhancement of insulin sensitivity.
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spelling pubmed-81577632021-06-03 Effect of exercise training on skeletal muscle protein expression in relation to insulin sensitivity: Per‐protocol analysis of a randomized controlled trial (GO‐ACTIWE) Bruhn, Lea Kjøbsted, Rasmus Quist, Jonas Salling Gram, Anne Sofie Rosenkilde, Mads Færch, Kristine Wojtaszewski, Jørgen F.P. Stallknecht, Bente Blond, Martin Bæk Physiol Rep Original Articles Exercise training improves peripheral insulin sensitivity and leads to molecular adaptations in the skeletal muscle. We investigated changes in the expression of key muscle proteins in the glucose metabolic pathway following active commuting by bike or leisure‐time exercise at two different intensities. In addition, potential associations between insulin sensitivity and muscle protein expression were examined. This per‐protocol analysis included 72 out of 130 physically inactive, healthy women and men (20–45 years) with overweight/obesity (BMI: 25–35 kg/m(2)) who completed 6 months of no intervention (CON, n = 12), active commuting by bike (BIKE, n = 14), or leisure‐time exercise of moderate (MOD, n = 28) or vigorous (VIG, n = 18) intensity. Exercise was prescribed 5 days/week with a weekly exercise energy expenditure of 1,600 kcal for women and 2,100 kcal for men. Insulin sensitivity was determined by a hyperinsulinemic euglycemic clamp and skeletal muscle biopsies were obtained from m. vastus lateralis and analyzed for protein expression at baseline and after 3 and 6 months of intervention. We found an increased expression of pyruvate dehydrogenase (PDH) in the exercise groups compared with the control group following 6 months of training. No differential effects were observed on the protein expression following moderate versus vigorous intensity exercise. In addition, we found a positive association between insulin sensitivity and the expression of glucose transporter type 4 as well as PDH. The positive association and the increase in expression of PDH after exercise training points toward a role for PDH in the training‐induced enhancement of insulin sensitivity. John Wiley and Sons Inc. 2021-05-27 /pmc/articles/PMC8157763/ /pubmed/34042297 http://dx.doi.org/10.14814/phy2.14850 Text en © 2021 The Authors. Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Bruhn, Lea
Kjøbsted, Rasmus
Quist, Jonas Salling
Gram, Anne Sofie
Rosenkilde, Mads
Færch, Kristine
Wojtaszewski, Jørgen F.P.
Stallknecht, Bente
Blond, Martin Bæk
Effect of exercise training on skeletal muscle protein expression in relation to insulin sensitivity: Per‐protocol analysis of a randomized controlled trial (GO‐ACTIWE)
title Effect of exercise training on skeletal muscle protein expression in relation to insulin sensitivity: Per‐protocol analysis of a randomized controlled trial (GO‐ACTIWE)
title_full Effect of exercise training on skeletal muscle protein expression in relation to insulin sensitivity: Per‐protocol analysis of a randomized controlled trial (GO‐ACTIWE)
title_fullStr Effect of exercise training on skeletal muscle protein expression in relation to insulin sensitivity: Per‐protocol analysis of a randomized controlled trial (GO‐ACTIWE)
title_full_unstemmed Effect of exercise training on skeletal muscle protein expression in relation to insulin sensitivity: Per‐protocol analysis of a randomized controlled trial (GO‐ACTIWE)
title_short Effect of exercise training on skeletal muscle protein expression in relation to insulin sensitivity: Per‐protocol analysis of a randomized controlled trial (GO‐ACTIWE)
title_sort effect of exercise training on skeletal muscle protein expression in relation to insulin sensitivity: per‐protocol analysis of a randomized controlled trial (go‐actiwe)
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8157763/
https://www.ncbi.nlm.nih.gov/pubmed/34042297
http://dx.doi.org/10.14814/phy2.14850
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