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Cytokine-Mediated Inflammation in the Oral Cavity and Its Effect on Lipid Nanocarriers

Topical drug administration to the oral mucosa proves to be a promising treatment alternative for inflammatory diseases. However, disease-related changes in the cell barrier must be considered when developing such delivery systems. This study aimed at investigating the changes in the lining mucosa c...

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Autores principales: Tetyczka, Carolin, Hartl, Sonja, Jeitler, Ramona, Absenger-Novak, Markus, Meindl, Claudia, Fröhlich, Eleonore, Riedl, Sabrina, Zweytick, Dagmar, Roblegg, Eva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8157841/
https://www.ncbi.nlm.nih.gov/pubmed/34070004
http://dx.doi.org/10.3390/nano11051330
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author Tetyczka, Carolin
Hartl, Sonja
Jeitler, Ramona
Absenger-Novak, Markus
Meindl, Claudia
Fröhlich, Eleonore
Riedl, Sabrina
Zweytick, Dagmar
Roblegg, Eva
author_facet Tetyczka, Carolin
Hartl, Sonja
Jeitler, Ramona
Absenger-Novak, Markus
Meindl, Claudia
Fröhlich, Eleonore
Riedl, Sabrina
Zweytick, Dagmar
Roblegg, Eva
author_sort Tetyczka, Carolin
collection PubMed
description Topical drug administration to the oral mucosa proves to be a promising treatment alternative for inflammatory diseases. However, disease-related changes in the cell barrier must be considered when developing such delivery systems. This study aimed at investigating the changes in the lining mucosa caused by inflammation and evaluating the consequences on drug delivery systems such as nanostructured lipid carriers (NLC). For this, TR146 cells were treated with inflammatory cytokines and bacterial components. Cell viability and integrity, reactive oxygen species (ROS), and interleukin (IL)-8 release were used as endpoints to assess inflammation. Translocation of phosphatidylserine, cytoskeletal arrangement, opening of desmosomes, and cell proliferation were examined. Transport studies with NLC were performed considering active and passive pathways. The results showed that IL-1ß and tumor necrosis factor α induced inflammation by increasing IL-8 and ROS production (22-fold and 2-fold). Morphologically, loss of cell–cell connections and formation of stress fibers and hyperplasia were observed. The charge of the cell membrane shifted from neutral to negative, which increased the absorption of NLC due to the repulsive interactions between the hydrophobic negative particles and the cell membrane on the one hand, and interactions with lipophilic membrane proteins such as caveolin on the other.
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spelling pubmed-81578412021-05-28 Cytokine-Mediated Inflammation in the Oral Cavity and Its Effect on Lipid Nanocarriers Tetyczka, Carolin Hartl, Sonja Jeitler, Ramona Absenger-Novak, Markus Meindl, Claudia Fröhlich, Eleonore Riedl, Sabrina Zweytick, Dagmar Roblegg, Eva Nanomaterials (Basel) Article Topical drug administration to the oral mucosa proves to be a promising treatment alternative for inflammatory diseases. However, disease-related changes in the cell barrier must be considered when developing such delivery systems. This study aimed at investigating the changes in the lining mucosa caused by inflammation and evaluating the consequences on drug delivery systems such as nanostructured lipid carriers (NLC). For this, TR146 cells were treated with inflammatory cytokines and bacterial components. Cell viability and integrity, reactive oxygen species (ROS), and interleukin (IL)-8 release were used as endpoints to assess inflammation. Translocation of phosphatidylserine, cytoskeletal arrangement, opening of desmosomes, and cell proliferation were examined. Transport studies with NLC were performed considering active and passive pathways. The results showed that IL-1ß and tumor necrosis factor α induced inflammation by increasing IL-8 and ROS production (22-fold and 2-fold). Morphologically, loss of cell–cell connections and formation of stress fibers and hyperplasia were observed. The charge of the cell membrane shifted from neutral to negative, which increased the absorption of NLC due to the repulsive interactions between the hydrophobic negative particles and the cell membrane on the one hand, and interactions with lipophilic membrane proteins such as caveolin on the other. MDPI 2021-05-18 /pmc/articles/PMC8157841/ /pubmed/34070004 http://dx.doi.org/10.3390/nano11051330 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tetyczka, Carolin
Hartl, Sonja
Jeitler, Ramona
Absenger-Novak, Markus
Meindl, Claudia
Fröhlich, Eleonore
Riedl, Sabrina
Zweytick, Dagmar
Roblegg, Eva
Cytokine-Mediated Inflammation in the Oral Cavity and Its Effect on Lipid Nanocarriers
title Cytokine-Mediated Inflammation in the Oral Cavity and Its Effect on Lipid Nanocarriers
title_full Cytokine-Mediated Inflammation in the Oral Cavity and Its Effect on Lipid Nanocarriers
title_fullStr Cytokine-Mediated Inflammation in the Oral Cavity and Its Effect on Lipid Nanocarriers
title_full_unstemmed Cytokine-Mediated Inflammation in the Oral Cavity and Its Effect on Lipid Nanocarriers
title_short Cytokine-Mediated Inflammation in the Oral Cavity and Its Effect on Lipid Nanocarriers
title_sort cytokine-mediated inflammation in the oral cavity and its effect on lipid nanocarriers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8157841/
https://www.ncbi.nlm.nih.gov/pubmed/34070004
http://dx.doi.org/10.3390/nano11051330
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