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Comparative Studies of Selected Criteria Enabling Optimization of the Extraction of Polar Biologically Active Compounds from Alfalfa with Supercritical Carbon Dioxide

The aim of this research was to provide crucial and useful data about the selection of the optimization criteria of supercritical carbon dioxide extraction of alfalfa at a quarter-technical plant. The correlation between more general output, including total phenolics and flavonoids content, and a mo...

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Autores principales: Wrona, Olga, Rafińska, Katarzyna, Krakowska-Sieprawska, Aneta, Buszewski, Bogusław
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8157860/
https://www.ncbi.nlm.nih.gov/pubmed/34069978
http://dx.doi.org/10.3390/molecules26102994
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author Wrona, Olga
Rafińska, Katarzyna
Krakowska-Sieprawska, Aneta
Buszewski, Bogusław
author_facet Wrona, Olga
Rafińska, Katarzyna
Krakowska-Sieprawska, Aneta
Buszewski, Bogusław
author_sort Wrona, Olga
collection PubMed
description The aim of this research was to provide crucial and useful data about the selection of the optimization criteria of supercritical carbon dioxide extraction of alfalfa at a quarter-technical plant. The correlation between more general output, including total phenolics and flavonoids content, and a more specified composition of polar constituents was extensively studied. In all alfalfa extracts, polar bioactive constituents were analyzed by both spectrometric (general output) and chromatographic (detailed output) analyses. Eight specific phenolic acids and nine flavonoids were determined. The most dominant were salicylic acid (221.41 µg g(−1)), ferulic acid (119.73 µg g(−1)), quercetin (2.23 µg g(−1)), and apigenin (2.60 µg g(−1)). For all seventeen analyzed compounds, response surface methodology and analysis of variance were used to provide the optimal conditions of supercritical fluid extraction for each individual constituent. The obtained data have shown that eight of those compounds have a similar range of optimal process parameters, being significantly analogous for optimization based on total flavonoid content.
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spelling pubmed-81578602021-05-28 Comparative Studies of Selected Criteria Enabling Optimization of the Extraction of Polar Biologically Active Compounds from Alfalfa with Supercritical Carbon Dioxide Wrona, Olga Rafińska, Katarzyna Krakowska-Sieprawska, Aneta Buszewski, Bogusław Molecules Article The aim of this research was to provide crucial and useful data about the selection of the optimization criteria of supercritical carbon dioxide extraction of alfalfa at a quarter-technical plant. The correlation between more general output, including total phenolics and flavonoids content, and a more specified composition of polar constituents was extensively studied. In all alfalfa extracts, polar bioactive constituents were analyzed by both spectrometric (general output) and chromatographic (detailed output) analyses. Eight specific phenolic acids and nine flavonoids were determined. The most dominant were salicylic acid (221.41 µg g(−1)), ferulic acid (119.73 µg g(−1)), quercetin (2.23 µg g(−1)), and apigenin (2.60 µg g(−1)). For all seventeen analyzed compounds, response surface methodology and analysis of variance were used to provide the optimal conditions of supercritical fluid extraction for each individual constituent. The obtained data have shown that eight of those compounds have a similar range of optimal process parameters, being significantly analogous for optimization based on total flavonoid content. MDPI 2021-05-18 /pmc/articles/PMC8157860/ /pubmed/34069978 http://dx.doi.org/10.3390/molecules26102994 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wrona, Olga
Rafińska, Katarzyna
Krakowska-Sieprawska, Aneta
Buszewski, Bogusław
Comparative Studies of Selected Criteria Enabling Optimization of the Extraction of Polar Biologically Active Compounds from Alfalfa with Supercritical Carbon Dioxide
title Comparative Studies of Selected Criteria Enabling Optimization of the Extraction of Polar Biologically Active Compounds from Alfalfa with Supercritical Carbon Dioxide
title_full Comparative Studies of Selected Criteria Enabling Optimization of the Extraction of Polar Biologically Active Compounds from Alfalfa with Supercritical Carbon Dioxide
title_fullStr Comparative Studies of Selected Criteria Enabling Optimization of the Extraction of Polar Biologically Active Compounds from Alfalfa with Supercritical Carbon Dioxide
title_full_unstemmed Comparative Studies of Selected Criteria Enabling Optimization of the Extraction of Polar Biologically Active Compounds from Alfalfa with Supercritical Carbon Dioxide
title_short Comparative Studies of Selected Criteria Enabling Optimization of the Extraction of Polar Biologically Active Compounds from Alfalfa with Supercritical Carbon Dioxide
title_sort comparative studies of selected criteria enabling optimization of the extraction of polar biologically active compounds from alfalfa with supercritical carbon dioxide
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8157860/
https://www.ncbi.nlm.nih.gov/pubmed/34069978
http://dx.doi.org/10.3390/molecules26102994
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