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Mapping the colorectal tumor microbiota

The gut microbiome in patients with colorectal cancer (CRC) is different than that of healthy controls. Previous studies have profiled the CRC tumor microbiome using a single biopsy. However, since the morphology and cellular subtype vary significantly within an individual tumor, the possibility of...

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Detalles Bibliográficos
Autores principales: Murphy, CL, Barrett, M, Pellanda, P, Killeen, S, McCourt, M, Andrews, E, O’ Riordain, M, Shanahan, F, O’Toole, Pw
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8158024/
https://www.ncbi.nlm.nih.gov/pubmed/34030582
http://dx.doi.org/10.1080/19490976.2021.1920657
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author Murphy, CL
Barrett, M
Pellanda, P
Killeen, S
McCourt, M
Andrews, E
O’ Riordain, M
Shanahan, F
O’Toole, Pw
author_facet Murphy, CL
Barrett, M
Pellanda, P
Killeen, S
McCourt, M
Andrews, E
O’ Riordain, M
Shanahan, F
O’Toole, Pw
author_sort Murphy, CL
collection PubMed
description The gut microbiome in patients with colorectal cancer (CRC) is different than that of healthy controls. Previous studies have profiled the CRC tumor microbiome using a single biopsy. However, since the morphology and cellular subtype vary significantly within an individual tumor, the possibility of sampling error arises for the microbiome within an individual tumor. To test this hypothesis, seven biopsies were taken from representative areas on and off the tumor in five patients with CRC. The microbiome composition was strikingly similar across all samples from an individual. The variation in microbiome alpha-diversity was significantly greater between individuals’ samples then within individuals. This is the first study, to our knowledge, that shows that the microbiome of an individual tumor is spatially homogeneous. Our finding strengthens the assumption that a single biopsy is representative of the entire tumor, and that microbiota changes are not limited to a specific area of the neoplasm.
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spelling pubmed-81580242021-07-09 Mapping the colorectal tumor microbiota Murphy, CL Barrett, M Pellanda, P Killeen, S McCourt, M Andrews, E O’ Riordain, M Shanahan, F O’Toole, Pw Gut Microbes Brief Report The gut microbiome in patients with colorectal cancer (CRC) is different than that of healthy controls. Previous studies have profiled the CRC tumor microbiome using a single biopsy. However, since the morphology and cellular subtype vary significantly within an individual tumor, the possibility of sampling error arises for the microbiome within an individual tumor. To test this hypothesis, seven biopsies were taken from representative areas on and off the tumor in five patients with CRC. The microbiome composition was strikingly similar across all samples from an individual. The variation in microbiome alpha-diversity was significantly greater between individuals’ samples then within individuals. This is the first study, to our knowledge, that shows that the microbiome of an individual tumor is spatially homogeneous. Our finding strengthens the assumption that a single biopsy is representative of the entire tumor, and that microbiota changes are not limited to a specific area of the neoplasm. Taylor & Francis 2021-05-25 /pmc/articles/PMC8158024/ /pubmed/34030582 http://dx.doi.org/10.1080/19490976.2021.1920657 Text en © 2021 The Author(s). Published with license by Taylor & Francis Group, LLC. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Brief Report
Murphy, CL
Barrett, M
Pellanda, P
Killeen, S
McCourt, M
Andrews, E
O’ Riordain, M
Shanahan, F
O’Toole, Pw
Mapping the colorectal tumor microbiota
title Mapping the colorectal tumor microbiota
title_full Mapping the colorectal tumor microbiota
title_fullStr Mapping the colorectal tumor microbiota
title_full_unstemmed Mapping the colorectal tumor microbiota
title_short Mapping the colorectal tumor microbiota
title_sort mapping the colorectal tumor microbiota
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8158024/
https://www.ncbi.nlm.nih.gov/pubmed/34030582
http://dx.doi.org/10.1080/19490976.2021.1920657
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