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Xylitol Inhibits Growth and Blocks Virulence in Serratia marcescens

Serratia marcescens is an opportunistic nosocomial pathogen and causes wound and burn infections. It shows high resistance to antibiotics and its pathogenicity is mediated by an arsenal of virulence factors. Another therapeutic option to such infections is targeting quorum sensing (QS), which contro...

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Autores principales: Khayyat, Ahdab N., Hegazy, Wael A. H., Shaldam, Moataz A., Mosbah, Rasha, Almalki, Ahmad J., Ibrahim, Tarek S., Khayat, Maan T., Khafagy, El-Sayed, Soliman, Wafaa E., Abbas, Hisham A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8158113/
https://www.ncbi.nlm.nih.gov/pubmed/34070043
http://dx.doi.org/10.3390/microorganisms9051083
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author Khayyat, Ahdab N.
Hegazy, Wael A. H.
Shaldam, Moataz A.
Mosbah, Rasha
Almalki, Ahmad J.
Ibrahim, Tarek S.
Khayat, Maan T.
Khafagy, El-Sayed
Soliman, Wafaa E.
Abbas, Hisham A.
author_facet Khayyat, Ahdab N.
Hegazy, Wael A. H.
Shaldam, Moataz A.
Mosbah, Rasha
Almalki, Ahmad J.
Ibrahim, Tarek S.
Khayat, Maan T.
Khafagy, El-Sayed
Soliman, Wafaa E.
Abbas, Hisham A.
author_sort Khayyat, Ahdab N.
collection PubMed
description Serratia marcescens is an opportunistic nosocomial pathogen and causes wound and burn infections. It shows high resistance to antibiotics and its pathogenicity is mediated by an arsenal of virulence factors. Another therapeutic option to such infections is targeting quorum sensing (QS), which controls the expression of different S. marcescens virulence factors. Prevention of QS can deprive S. marcescens from its bacterial virulence without applying stress on the bacterial growth and facilitates the eradication of the bacteria by immunity. The objective of the current study is to explore the antimicrobial and antivirulence activities of xylitol against S. marcescens. Xylitol could inhibit the growth of S. marcescens. Sub-inhibitory concentrations of xylitol could inhibit biofilm formation, reduce prodigiosin production, and completely block protease activity. Moreover, xylitol decreased swimming motility, swarming motility and increased the sensitivity to hydrogen peroxide. The expression of rsmA, pigP, flhC, flhD fimA, fimC, shlA bsmB, and rssB genes that regulate virulence factor production was significantly downregulated by xylitol. In silico study showed that xylitol could bind with the SmaR receptor by hydrophobic interaction and hydrogen bonding, and interfere with the binding of the natural ligand with SmaR receptor. An in vivo mice survival test confirmed the ability of xylitol to protect mice against the virulence of S. marcescens. In conclusion, xylitol is a growth and virulence inhibitor in S. marcescens and can be employed for the treatment of S. marcescens wound and burn infections.
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spelling pubmed-81581132021-05-28 Xylitol Inhibits Growth and Blocks Virulence in Serratia marcescens Khayyat, Ahdab N. Hegazy, Wael A. H. Shaldam, Moataz A. Mosbah, Rasha Almalki, Ahmad J. Ibrahim, Tarek S. Khayat, Maan T. Khafagy, El-Sayed Soliman, Wafaa E. Abbas, Hisham A. Microorganisms Article Serratia marcescens is an opportunistic nosocomial pathogen and causes wound and burn infections. It shows high resistance to antibiotics and its pathogenicity is mediated by an arsenal of virulence factors. Another therapeutic option to such infections is targeting quorum sensing (QS), which controls the expression of different S. marcescens virulence factors. Prevention of QS can deprive S. marcescens from its bacterial virulence without applying stress on the bacterial growth and facilitates the eradication of the bacteria by immunity. The objective of the current study is to explore the antimicrobial and antivirulence activities of xylitol against S. marcescens. Xylitol could inhibit the growth of S. marcescens. Sub-inhibitory concentrations of xylitol could inhibit biofilm formation, reduce prodigiosin production, and completely block protease activity. Moreover, xylitol decreased swimming motility, swarming motility and increased the sensitivity to hydrogen peroxide. The expression of rsmA, pigP, flhC, flhD fimA, fimC, shlA bsmB, and rssB genes that regulate virulence factor production was significantly downregulated by xylitol. In silico study showed that xylitol could bind with the SmaR receptor by hydrophobic interaction and hydrogen bonding, and interfere with the binding of the natural ligand with SmaR receptor. An in vivo mice survival test confirmed the ability of xylitol to protect mice against the virulence of S. marcescens. In conclusion, xylitol is a growth and virulence inhibitor in S. marcescens and can be employed for the treatment of S. marcescens wound and burn infections. MDPI 2021-05-18 /pmc/articles/PMC8158113/ /pubmed/34070043 http://dx.doi.org/10.3390/microorganisms9051083 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Khayyat, Ahdab N.
Hegazy, Wael A. H.
Shaldam, Moataz A.
Mosbah, Rasha
Almalki, Ahmad J.
Ibrahim, Tarek S.
Khayat, Maan T.
Khafagy, El-Sayed
Soliman, Wafaa E.
Abbas, Hisham A.
Xylitol Inhibits Growth and Blocks Virulence in Serratia marcescens
title Xylitol Inhibits Growth and Blocks Virulence in Serratia marcescens
title_full Xylitol Inhibits Growth and Blocks Virulence in Serratia marcescens
title_fullStr Xylitol Inhibits Growth and Blocks Virulence in Serratia marcescens
title_full_unstemmed Xylitol Inhibits Growth and Blocks Virulence in Serratia marcescens
title_short Xylitol Inhibits Growth and Blocks Virulence in Serratia marcescens
title_sort xylitol inhibits growth and blocks virulence in serratia marcescens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8158113/
https://www.ncbi.nlm.nih.gov/pubmed/34070043
http://dx.doi.org/10.3390/microorganisms9051083
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