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Impacts of the Type I Toxin–Antitoxin System, SprG1/SprF1, on Staphylococcus aureus Gene Expression

Type I toxin–antitoxin (TA) systems are widespread genetic modules in bacterial genomes. They express toxic peptides whose overexpression leads to growth arrest or cell death, whereas antitoxins regulate the expression of toxins, acting as labile antisense RNAs. The Staphylococcus aureus (S. aureus)...

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Autores principales: Chlebicka, Kinga, Bonar, Emilia, Suder, Piotr, Ostyn, Emeline, Felden, Brice, Wladyka, Benedykt, Pinel-Marie, Marie-Laure
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8158120/
https://www.ncbi.nlm.nih.gov/pubmed/34070083
http://dx.doi.org/10.3390/genes12050770
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author Chlebicka, Kinga
Bonar, Emilia
Suder, Piotr
Ostyn, Emeline
Felden, Brice
Wladyka, Benedykt
Pinel-Marie, Marie-Laure
author_facet Chlebicka, Kinga
Bonar, Emilia
Suder, Piotr
Ostyn, Emeline
Felden, Brice
Wladyka, Benedykt
Pinel-Marie, Marie-Laure
author_sort Chlebicka, Kinga
collection PubMed
description Type I toxin–antitoxin (TA) systems are widespread genetic modules in bacterial genomes. They express toxic peptides whose overexpression leads to growth arrest or cell death, whereas antitoxins regulate the expression of toxins, acting as labile antisense RNAs. The Staphylococcus aureus (S. aureus) genome contains and expresses several functional type I TA systems, but their biological functions remain unclear. Here, we addressed and challenged experimentally, by proteomics, if the type I TA system, the SprG1/SprF1 pair, influences the overall gene expression in S. aureus. Deleted and complemented S. aureus strains were analyzed for their proteomes, both intracellular and extracellular, during growth. Comparison of intracellular proteomes among the strains points to the SprF1 antitoxin as moderately downregulating protein expression. In the strain naturally expressing the SprG1 toxin, cytoplasmic proteins are excreted into the medium, but this is not due to unspecific cell leakages. Such a toxin-driven release of the cytoplasmic proteins may modulate the host inflammatory response that, in turn, could amplify the S. aureus infection spread.
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spelling pubmed-81581202021-05-28 Impacts of the Type I Toxin–Antitoxin System, SprG1/SprF1, on Staphylococcus aureus Gene Expression Chlebicka, Kinga Bonar, Emilia Suder, Piotr Ostyn, Emeline Felden, Brice Wladyka, Benedykt Pinel-Marie, Marie-Laure Genes (Basel) Article Type I toxin–antitoxin (TA) systems are widespread genetic modules in bacterial genomes. They express toxic peptides whose overexpression leads to growth arrest or cell death, whereas antitoxins regulate the expression of toxins, acting as labile antisense RNAs. The Staphylococcus aureus (S. aureus) genome contains and expresses several functional type I TA systems, but their biological functions remain unclear. Here, we addressed and challenged experimentally, by proteomics, if the type I TA system, the SprG1/SprF1 pair, influences the overall gene expression in S. aureus. Deleted and complemented S. aureus strains were analyzed for their proteomes, both intracellular and extracellular, during growth. Comparison of intracellular proteomes among the strains points to the SprF1 antitoxin as moderately downregulating protein expression. In the strain naturally expressing the SprG1 toxin, cytoplasmic proteins are excreted into the medium, but this is not due to unspecific cell leakages. Such a toxin-driven release of the cytoplasmic proteins may modulate the host inflammatory response that, in turn, could amplify the S. aureus infection spread. MDPI 2021-05-18 /pmc/articles/PMC8158120/ /pubmed/34070083 http://dx.doi.org/10.3390/genes12050770 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chlebicka, Kinga
Bonar, Emilia
Suder, Piotr
Ostyn, Emeline
Felden, Brice
Wladyka, Benedykt
Pinel-Marie, Marie-Laure
Impacts of the Type I Toxin–Antitoxin System, SprG1/SprF1, on Staphylococcus aureus Gene Expression
title Impacts of the Type I Toxin–Antitoxin System, SprG1/SprF1, on Staphylococcus aureus Gene Expression
title_full Impacts of the Type I Toxin–Antitoxin System, SprG1/SprF1, on Staphylococcus aureus Gene Expression
title_fullStr Impacts of the Type I Toxin–Antitoxin System, SprG1/SprF1, on Staphylococcus aureus Gene Expression
title_full_unstemmed Impacts of the Type I Toxin–Antitoxin System, SprG1/SprF1, on Staphylococcus aureus Gene Expression
title_short Impacts of the Type I Toxin–Antitoxin System, SprG1/SprF1, on Staphylococcus aureus Gene Expression
title_sort impacts of the type i toxin–antitoxin system, sprg1/sprf1, on staphylococcus aureus gene expression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8158120/
https://www.ncbi.nlm.nih.gov/pubmed/34070083
http://dx.doi.org/10.3390/genes12050770
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