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MicroRNA Profiles of Maternal and Neonatal Endothelial Progenitor Cells in Preeclampsia

Preeclampsia is associated with an increased cardiovascular morbidity of mother and offspring, thus contributing to a substantial burden in women and children’s health. It has been proven that endothelial progenitor cell (EPC) numbers and functional characteristics are impaired in cardiovascular dis...

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Autores principales: Brodowski, Lars, Schröder-Heurich, Bianca, von Hardenberg, Sandra, Richter, Katja, von Kaisenberg, Constantin S., Dittrich-Breiholz, Oliver, Meyer, Nadia, Dörk, Thilo, von Versen-Höynck, Frauke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8158476/
https://www.ncbi.nlm.nih.gov/pubmed/34070163
http://dx.doi.org/10.3390/ijms22105320
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author Brodowski, Lars
Schröder-Heurich, Bianca
von Hardenberg, Sandra
Richter, Katja
von Kaisenberg, Constantin S.
Dittrich-Breiholz, Oliver
Meyer, Nadia
Dörk, Thilo
von Versen-Höynck, Frauke
author_facet Brodowski, Lars
Schröder-Heurich, Bianca
von Hardenberg, Sandra
Richter, Katja
von Kaisenberg, Constantin S.
Dittrich-Breiholz, Oliver
Meyer, Nadia
Dörk, Thilo
von Versen-Höynck, Frauke
author_sort Brodowski, Lars
collection PubMed
description Preeclampsia is associated with an increased cardiovascular morbidity of mother and offspring, thus contributing to a substantial burden in women and children’s health. It has been proven that endothelial progenitor cell (EPC) numbers and functional characteristics are impaired in cardiovascular disease and preeclampsia, although causative factors for the latter have remained elusive. MicroRNA (miRNA) modifications are a potential mechanism through which exposure to an altered environment translates into the development of chronic disease. In this study, we examined whether development of preeclampsia corresponds to alterations of miRNAs in maternal- and cord-blood-derived EPC. To test this end, we analyzed maternal and neonatal miRNAs via RNA sequencing from endothelial cells of preeclamptic and healthy controls in different cell culture passages. We were able to demonstrate differentially represented miRNAs in all groups. Hsa-miR-1270 showed significantly different levels in cord blood EPC from preeclampsia versus control and was negatively correlated with mRNA levels of its predicted targets ANGPTL7 and TFRC. Transfection with an hsa-miR-1270 inhibitor decreased the tube formation capacity and chemotactic motility but did not change proliferation in vitro. Target predictions and gene set enrichment analyses identified alternative splicing as a significantly enriched pathway for hsa-miR-1270. The top miRNAs in three other groups were predicted to target transcriptional and developmental pathways. Here, we showed for the first time significantly different levels of miRNAs and differently represented mRNA levels of predicted target genes in EPC derived from preeclampsia. Understanding the effects of preeclampsia on the epigenetic mechanisms of EPC will be crucial and may provide initial insights for further evaluation of the benefits of therapies targeting this cell population.
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spelling pubmed-81584762021-05-28 MicroRNA Profiles of Maternal and Neonatal Endothelial Progenitor Cells in Preeclampsia Brodowski, Lars Schröder-Heurich, Bianca von Hardenberg, Sandra Richter, Katja von Kaisenberg, Constantin S. Dittrich-Breiholz, Oliver Meyer, Nadia Dörk, Thilo von Versen-Höynck, Frauke Int J Mol Sci Article Preeclampsia is associated with an increased cardiovascular morbidity of mother and offspring, thus contributing to a substantial burden in women and children’s health. It has been proven that endothelial progenitor cell (EPC) numbers and functional characteristics are impaired in cardiovascular disease and preeclampsia, although causative factors for the latter have remained elusive. MicroRNA (miRNA) modifications are a potential mechanism through which exposure to an altered environment translates into the development of chronic disease. In this study, we examined whether development of preeclampsia corresponds to alterations of miRNAs in maternal- and cord-blood-derived EPC. To test this end, we analyzed maternal and neonatal miRNAs via RNA sequencing from endothelial cells of preeclamptic and healthy controls in different cell culture passages. We were able to demonstrate differentially represented miRNAs in all groups. Hsa-miR-1270 showed significantly different levels in cord blood EPC from preeclampsia versus control and was negatively correlated with mRNA levels of its predicted targets ANGPTL7 and TFRC. Transfection with an hsa-miR-1270 inhibitor decreased the tube formation capacity and chemotactic motility but did not change proliferation in vitro. Target predictions and gene set enrichment analyses identified alternative splicing as a significantly enriched pathway for hsa-miR-1270. The top miRNAs in three other groups were predicted to target transcriptional and developmental pathways. Here, we showed for the first time significantly different levels of miRNAs and differently represented mRNA levels of predicted target genes in EPC derived from preeclampsia. Understanding the effects of preeclampsia on the epigenetic mechanisms of EPC will be crucial and may provide initial insights for further evaluation of the benefits of therapies targeting this cell population. MDPI 2021-05-18 /pmc/articles/PMC8158476/ /pubmed/34070163 http://dx.doi.org/10.3390/ijms22105320 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Brodowski, Lars
Schröder-Heurich, Bianca
von Hardenberg, Sandra
Richter, Katja
von Kaisenberg, Constantin S.
Dittrich-Breiholz, Oliver
Meyer, Nadia
Dörk, Thilo
von Versen-Höynck, Frauke
MicroRNA Profiles of Maternal and Neonatal Endothelial Progenitor Cells in Preeclampsia
title MicroRNA Profiles of Maternal and Neonatal Endothelial Progenitor Cells in Preeclampsia
title_full MicroRNA Profiles of Maternal and Neonatal Endothelial Progenitor Cells in Preeclampsia
title_fullStr MicroRNA Profiles of Maternal and Neonatal Endothelial Progenitor Cells in Preeclampsia
title_full_unstemmed MicroRNA Profiles of Maternal and Neonatal Endothelial Progenitor Cells in Preeclampsia
title_short MicroRNA Profiles of Maternal and Neonatal Endothelial Progenitor Cells in Preeclampsia
title_sort microrna profiles of maternal and neonatal endothelial progenitor cells in preeclampsia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8158476/
https://www.ncbi.nlm.nih.gov/pubmed/34070163
http://dx.doi.org/10.3390/ijms22105320
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