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In Vitro Antiviral Activity of α-Mangostin against Dengue Virus Serotype-2 (DENV-2)
Dengue virus (DENV), a member of the family Flaviviridae, is a threat for global health as it infects more than 100 million people yearly. Approved antiviral therapies or vaccines for the treatment or prevention of DENV infections are not available. In the present study, natural compounds were scree...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8158742/ https://www.ncbi.nlm.nih.gov/pubmed/34069351 http://dx.doi.org/10.3390/molecules26103016 |
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author | Panda, Kingshuk Alagarasu, Kalichamy Patil, Poonam Agrawal, Megha More, Ashwini Kumar, Naveen V. Mainkar, Prathama S. Parashar, Deepti Cherian, Sarah |
author_facet | Panda, Kingshuk Alagarasu, Kalichamy Patil, Poonam Agrawal, Megha More, Ashwini Kumar, Naveen V. Mainkar, Prathama S. Parashar, Deepti Cherian, Sarah |
author_sort | Panda, Kingshuk |
collection | PubMed |
description | Dengue virus (DENV), a member of the family Flaviviridae, is a threat for global health as it infects more than 100 million people yearly. Approved antiviral therapies or vaccines for the treatment or prevention of DENV infections are not available. In the present study, natural compounds were screened for their antiviral activity against DENV by in vitro cell line-based assay. α-Mangostin, a xanthanoid, was observed to exert antiviral activity against DENV-2 under pre-, co- and post-treatment testing conditions. The antiviral activity was determined by foci forming unit (FFU) assay, quantitative RT-PCR and cell-based immunofluorescence assay (IFA). A complete inhibition of DENV-2 was observed at 8 µM under the co-treatment condition. The possible inhibitory mechanism of α-Mangostin was also determined by docking studies. The molecular docking experiments indicate that α-Mangostin can interact with multiple DENV protein targets such as the NS5 methyltransferase, NS2B-NS3 protease and the glycoprotein E. The in vitro and in silico findings suggest that α-Mangostin possesses the ability to suppress DENV-2 production at different stages of its replication cycle and might act as a prophylactic/therapeutic agent against DENV-2. |
format | Online Article Text |
id | pubmed-8158742 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-81587422021-05-28 In Vitro Antiviral Activity of α-Mangostin against Dengue Virus Serotype-2 (DENV-2) Panda, Kingshuk Alagarasu, Kalichamy Patil, Poonam Agrawal, Megha More, Ashwini Kumar, Naveen V. Mainkar, Prathama S. Parashar, Deepti Cherian, Sarah Molecules Article Dengue virus (DENV), a member of the family Flaviviridae, is a threat for global health as it infects more than 100 million people yearly. Approved antiviral therapies or vaccines for the treatment or prevention of DENV infections are not available. In the present study, natural compounds were screened for their antiviral activity against DENV by in vitro cell line-based assay. α-Mangostin, a xanthanoid, was observed to exert antiviral activity against DENV-2 under pre-, co- and post-treatment testing conditions. The antiviral activity was determined by foci forming unit (FFU) assay, quantitative RT-PCR and cell-based immunofluorescence assay (IFA). A complete inhibition of DENV-2 was observed at 8 µM under the co-treatment condition. The possible inhibitory mechanism of α-Mangostin was also determined by docking studies. The molecular docking experiments indicate that α-Mangostin can interact with multiple DENV protein targets such as the NS5 methyltransferase, NS2B-NS3 protease and the glycoprotein E. The in vitro and in silico findings suggest that α-Mangostin possesses the ability to suppress DENV-2 production at different stages of its replication cycle and might act as a prophylactic/therapeutic agent against DENV-2. MDPI 2021-05-19 /pmc/articles/PMC8158742/ /pubmed/34069351 http://dx.doi.org/10.3390/molecules26103016 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Panda, Kingshuk Alagarasu, Kalichamy Patil, Poonam Agrawal, Megha More, Ashwini Kumar, Naveen V. Mainkar, Prathama S. Parashar, Deepti Cherian, Sarah In Vitro Antiviral Activity of α-Mangostin against Dengue Virus Serotype-2 (DENV-2) |
title | In Vitro Antiviral Activity of α-Mangostin against Dengue Virus Serotype-2 (DENV-2) |
title_full | In Vitro Antiviral Activity of α-Mangostin against Dengue Virus Serotype-2 (DENV-2) |
title_fullStr | In Vitro Antiviral Activity of α-Mangostin against Dengue Virus Serotype-2 (DENV-2) |
title_full_unstemmed | In Vitro Antiviral Activity of α-Mangostin against Dengue Virus Serotype-2 (DENV-2) |
title_short | In Vitro Antiviral Activity of α-Mangostin against Dengue Virus Serotype-2 (DENV-2) |
title_sort | in vitro antiviral activity of α-mangostin against dengue virus serotype-2 (denv-2) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8158742/ https://www.ncbi.nlm.nih.gov/pubmed/34069351 http://dx.doi.org/10.3390/molecules26103016 |
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