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Surfactant-Free Decellularization of Porcine Aortic Tissue by Subcritical Dimethyl Ether
[Image: see text] Porcine aortic tissue was decellularized by subcritical dimethyl ether (DME) used as an alternative to the surfactant sodium dodecyl sulfate. The process included three steps. For the first step, lipids were extracted from the porcine aorta using subcritical DME at 23 °C with a DME...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8158793/ https://www.ncbi.nlm.nih.gov/pubmed/34056489 http://dx.doi.org/10.1021/acsomega.1c01549 |
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author | Kanda, Hideki Ando, Daigo Hoshino, Rintaro Yamamoto, Tetsuya Wahyudiono, Suzuki, Shogo Shinohara, Satoshi Goto, Motonobu |
author_facet | Kanda, Hideki Ando, Daigo Hoshino, Rintaro Yamamoto, Tetsuya Wahyudiono, Suzuki, Shogo Shinohara, Satoshi Goto, Motonobu |
author_sort | Kanda, Hideki |
collection | PubMed |
description | [Image: see text] Porcine aortic tissue was decellularized by subcritical dimethyl ether (DME) used as an alternative to the surfactant sodium dodecyl sulfate. The process included three steps. For the first step, lipids were extracted from the porcine aorta using subcritical DME at 23 °C with a DME pressure of 0.56 MPa. Next, DME was evaporated from the aorta under atmospheric pressure and temperature. The second step involved DNA fragmentation by DNase, which was primarily identical to the common method. For the third step, similar to the common method, DNA fragments were removed by washing with water and ethanol. After 3 days of DNase treatment, the amount of DNA remaining in the porcine aorta was 40 ng/dry-mg, which was lower than the standard value of 50 ng/mg-dry. Hematoxylin and eosin staining showed that most cell nuclei were removed from the aorta. These results demonstrate that subcritical DME eliminates the need to utilize surfactants. |
format | Online Article Text |
id | pubmed-8158793 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-81587932021-05-28 Surfactant-Free Decellularization of Porcine Aortic Tissue by Subcritical Dimethyl Ether Kanda, Hideki Ando, Daigo Hoshino, Rintaro Yamamoto, Tetsuya Wahyudiono, Suzuki, Shogo Shinohara, Satoshi Goto, Motonobu ACS Omega [Image: see text] Porcine aortic tissue was decellularized by subcritical dimethyl ether (DME) used as an alternative to the surfactant sodium dodecyl sulfate. The process included three steps. For the first step, lipids were extracted from the porcine aorta using subcritical DME at 23 °C with a DME pressure of 0.56 MPa. Next, DME was evaporated from the aorta under atmospheric pressure and temperature. The second step involved DNA fragmentation by DNase, which was primarily identical to the common method. For the third step, similar to the common method, DNA fragments were removed by washing with water and ethanol. After 3 days of DNase treatment, the amount of DNA remaining in the porcine aorta was 40 ng/dry-mg, which was lower than the standard value of 50 ng/mg-dry. Hematoxylin and eosin staining showed that most cell nuclei were removed from the aorta. These results demonstrate that subcritical DME eliminates the need to utilize surfactants. American Chemical Society 2021-05-14 /pmc/articles/PMC8158793/ /pubmed/34056489 http://dx.doi.org/10.1021/acsomega.1c01549 Text en © 2021 The Authors. Published by American Chemical Society Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Kanda, Hideki Ando, Daigo Hoshino, Rintaro Yamamoto, Tetsuya Wahyudiono, Suzuki, Shogo Shinohara, Satoshi Goto, Motonobu Surfactant-Free Decellularization of Porcine Aortic Tissue by Subcritical Dimethyl Ether |
title | Surfactant-Free Decellularization of Porcine Aortic
Tissue by Subcritical Dimethyl Ether |
title_full | Surfactant-Free Decellularization of Porcine Aortic
Tissue by Subcritical Dimethyl Ether |
title_fullStr | Surfactant-Free Decellularization of Porcine Aortic
Tissue by Subcritical Dimethyl Ether |
title_full_unstemmed | Surfactant-Free Decellularization of Porcine Aortic
Tissue by Subcritical Dimethyl Ether |
title_short | Surfactant-Free Decellularization of Porcine Aortic
Tissue by Subcritical Dimethyl Ether |
title_sort | surfactant-free decellularization of porcine aortic
tissue by subcritical dimethyl ether |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8158793/ https://www.ncbi.nlm.nih.gov/pubmed/34056489 http://dx.doi.org/10.1021/acsomega.1c01549 |
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