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Förster Resonance Energy Transfer Nanoplatform Based on Recognition-Induced Fusion/Fission of DNA Mixed Micelles for Nucleic Acid Sensing

[Image: see text] The dynamic nature of micellar nanostructures is employed to form a self-assembled Förster resonance energy transfer (FRET) nanoplatform for enhanced sensing of DNA. The platform consists of lipid oligonucleotide FRET probes incorporated into micellar scaffolds, where single recogn...

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Autores principales: Vafaei, Setareh, Allabush, Francia, Tabaei, Seyed R., Male, Louise, Dafforn, Timothy R., Tucker, James H. R., Mendes, Paula M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8158853/
https://www.ncbi.nlm.nih.gov/pubmed/33961404
http://dx.doi.org/10.1021/acsnano.1c00156
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author Vafaei, Setareh
Allabush, Francia
Tabaei, Seyed R.
Male, Louise
Dafforn, Timothy R.
Tucker, James H. R.
Mendes, Paula M.
author_facet Vafaei, Setareh
Allabush, Francia
Tabaei, Seyed R.
Male, Louise
Dafforn, Timothy R.
Tucker, James H. R.
Mendes, Paula M.
author_sort Vafaei, Setareh
collection PubMed
description [Image: see text] The dynamic nature of micellar nanostructures is employed to form a self-assembled Förster resonance energy transfer (FRET) nanoplatform for enhanced sensing of DNA. The platform consists of lipid oligonucleotide FRET probes incorporated into micellar scaffolds, where single recognition events result in fusion and fission of DNA mixed micelles, triggering the fluorescence response of multiple rather than a single FRET pair. In comparison to conventional FRET substrates where a single donor interacts with a single acceptor, the micellar multiplex FRET system showed ∼20- and ∼3-fold enhancements in the limit of detection and FRET efficiency, respectively. This supramolecular signal amplification approach could potentially be used to improve FRET-based diagnostic assays of nucleic acid and non-DNA based targets.
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spelling pubmed-81588532021-05-28 Förster Resonance Energy Transfer Nanoplatform Based on Recognition-Induced Fusion/Fission of DNA Mixed Micelles for Nucleic Acid Sensing Vafaei, Setareh Allabush, Francia Tabaei, Seyed R. Male, Louise Dafforn, Timothy R. Tucker, James H. R. Mendes, Paula M. ACS Nano [Image: see text] The dynamic nature of micellar nanostructures is employed to form a self-assembled Förster resonance energy transfer (FRET) nanoplatform for enhanced sensing of DNA. The platform consists of lipid oligonucleotide FRET probes incorporated into micellar scaffolds, where single recognition events result in fusion and fission of DNA mixed micelles, triggering the fluorescence response of multiple rather than a single FRET pair. In comparison to conventional FRET substrates where a single donor interacts with a single acceptor, the micellar multiplex FRET system showed ∼20- and ∼3-fold enhancements in the limit of detection and FRET efficiency, respectively. This supramolecular signal amplification approach could potentially be used to improve FRET-based diagnostic assays of nucleic acid and non-DNA based targets. American Chemical Society 2021-05-07 2021-05-25 /pmc/articles/PMC8158853/ /pubmed/33961404 http://dx.doi.org/10.1021/acsnano.1c00156 Text en © 2021 The Authors. Published by American Chemical Society Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Vafaei, Setareh
Allabush, Francia
Tabaei, Seyed R.
Male, Louise
Dafforn, Timothy R.
Tucker, James H. R.
Mendes, Paula M.
Förster Resonance Energy Transfer Nanoplatform Based on Recognition-Induced Fusion/Fission of DNA Mixed Micelles for Nucleic Acid Sensing
title Förster Resonance Energy Transfer Nanoplatform Based on Recognition-Induced Fusion/Fission of DNA Mixed Micelles for Nucleic Acid Sensing
title_full Förster Resonance Energy Transfer Nanoplatform Based on Recognition-Induced Fusion/Fission of DNA Mixed Micelles for Nucleic Acid Sensing
title_fullStr Förster Resonance Energy Transfer Nanoplatform Based on Recognition-Induced Fusion/Fission of DNA Mixed Micelles for Nucleic Acid Sensing
title_full_unstemmed Förster Resonance Energy Transfer Nanoplatform Based on Recognition-Induced Fusion/Fission of DNA Mixed Micelles for Nucleic Acid Sensing
title_short Förster Resonance Energy Transfer Nanoplatform Based on Recognition-Induced Fusion/Fission of DNA Mixed Micelles for Nucleic Acid Sensing
title_sort förster resonance energy transfer nanoplatform based on recognition-induced fusion/fission of dna mixed micelles for nucleic acid sensing
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8158853/
https://www.ncbi.nlm.nih.gov/pubmed/33961404
http://dx.doi.org/10.1021/acsnano.1c00156
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