A new species of Chlamydia isolated from Siamese crocodiles (Crocodylus siamensis)

Chlamydia is a known pathogen in both saltwater and freshwater crocodiles. However, the exact species/strain has not been clearly identified. In this study, we successfully cultivated Siamese crocodile Chlamydia in McCoy cells at a temperature of 30°C. Electron microscopy; phylogeny based on nine co...

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Autores principales: Chaiwattanarungruengpaisan, Somjit, Thongdee, Metawee, Anuntakarun, Songtham, Payungporn, Sunchai, Arya, Nlin, Punchukrang, Apichart, Ramasoota, Pongrama, Singhakaew, Sombat, Atithep, Thassanant, Sariya, Ladawan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8158970/
https://www.ncbi.nlm.nih.gov/pubmed/34043689
http://dx.doi.org/10.1371/journal.pone.0252081
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author Chaiwattanarungruengpaisan, Somjit
Thongdee, Metawee
Anuntakarun, Songtham
Payungporn, Sunchai
Arya, Nlin
Punchukrang, Apichart
Ramasoota, Pongrama
Singhakaew, Sombat
Atithep, Thassanant
Sariya, Ladawan
author_facet Chaiwattanarungruengpaisan, Somjit
Thongdee, Metawee
Anuntakarun, Songtham
Payungporn, Sunchai
Arya, Nlin
Punchukrang, Apichart
Ramasoota, Pongrama
Singhakaew, Sombat
Atithep, Thassanant
Sariya, Ladawan
author_sort Chaiwattanarungruengpaisan, Somjit
collection PubMed
description Chlamydia is a known pathogen in both saltwater and freshwater crocodiles. However, the exact species/strain has not been clearly identified. In this study, we successfully cultivated Siamese crocodile Chlamydia in McCoy cells at a temperature of 30°C. Electron microscopy; phylogeny based on nine conserved taxonomically informative markers, on ompA, or on seven housekeeping genes; and whole-genome sequencing and analysis of the isolate confirmed the identity of the isolate as a new member of the genus Chlamydia, a new species that we name Chlamydia crocodili.
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spelling pubmed-81589702021-06-10 A new species of Chlamydia isolated from Siamese crocodiles (Crocodylus siamensis) Chaiwattanarungruengpaisan, Somjit Thongdee, Metawee Anuntakarun, Songtham Payungporn, Sunchai Arya, Nlin Punchukrang, Apichart Ramasoota, Pongrama Singhakaew, Sombat Atithep, Thassanant Sariya, Ladawan PLoS One Research Article Chlamydia is a known pathogen in both saltwater and freshwater crocodiles. However, the exact species/strain has not been clearly identified. In this study, we successfully cultivated Siamese crocodile Chlamydia in McCoy cells at a temperature of 30°C. Electron microscopy; phylogeny based on nine conserved taxonomically informative markers, on ompA, or on seven housekeeping genes; and whole-genome sequencing and analysis of the isolate confirmed the identity of the isolate as a new member of the genus Chlamydia, a new species that we name Chlamydia crocodili. Public Library of Science 2021-05-27 /pmc/articles/PMC8158970/ /pubmed/34043689 http://dx.doi.org/10.1371/journal.pone.0252081 Text en © 2021 Chaiwattanarungruengpaisan et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Chaiwattanarungruengpaisan, Somjit
Thongdee, Metawee
Anuntakarun, Songtham
Payungporn, Sunchai
Arya, Nlin
Punchukrang, Apichart
Ramasoota, Pongrama
Singhakaew, Sombat
Atithep, Thassanant
Sariya, Ladawan
A new species of Chlamydia isolated from Siamese crocodiles (Crocodylus siamensis)
title A new species of Chlamydia isolated from Siamese crocodiles (Crocodylus siamensis)
title_full A new species of Chlamydia isolated from Siamese crocodiles (Crocodylus siamensis)
title_fullStr A new species of Chlamydia isolated from Siamese crocodiles (Crocodylus siamensis)
title_full_unstemmed A new species of Chlamydia isolated from Siamese crocodiles (Crocodylus siamensis)
title_short A new species of Chlamydia isolated from Siamese crocodiles (Crocodylus siamensis)
title_sort new species of chlamydia isolated from siamese crocodiles (crocodylus siamensis)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8158970/
https://www.ncbi.nlm.nih.gov/pubmed/34043689
http://dx.doi.org/10.1371/journal.pone.0252081
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