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One stone, three birds: one AIEgen with three colors for fast differentiation of three pathogens
Visually identifying pathogens favors rapid diagnosis at the point-of-care testing level. Here, we developed a microenvironment-sensitive aggregation-induced emission luminogen (AIEgen), namely IQ–Cm, for achieving fast discrimination of Gram-negative bacteria, Gram-positive bacteria and fungi by th...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8159167/ https://www.ncbi.nlm.nih.gov/pubmed/34122928 http://dx.doi.org/10.1039/d0sc00256a |
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author | Zhou, Chengcheng Jiang, Meijuan Du, Jian Bai, Haotian Shan, Guogang Kwok, Ryan T. K. Chau, Joe H. C. Zhang, Jun Lam, Jacky W. Y. Huang, Peng Tang, Ben Zhong |
author_facet | Zhou, Chengcheng Jiang, Meijuan Du, Jian Bai, Haotian Shan, Guogang Kwok, Ryan T. K. Chau, Joe H. C. Zhang, Jun Lam, Jacky W. Y. Huang, Peng Tang, Ben Zhong |
author_sort | Zhou, Chengcheng |
collection | PubMed |
description | Visually identifying pathogens favors rapid diagnosis at the point-of-care testing level. Here, we developed a microenvironment-sensitive aggregation-induced emission luminogen (AIEgen), namely IQ–Cm, for achieving fast discrimination of Gram-negative bacteria, Gram-positive bacteria and fungi by the naked-eye. With a twisted donor–acceptor and multi-rotor structure, IQ–Cm shows twisted intramolecular charge transfer (TICT) and AIE properties with sensitive fluorescence color response to the microenvironment of pathogens. Driven by the intrinsic structural differences of pathogens, IQ–Cm with a cationic isoquinolinium moiety and a membrane-active coumarin unit as the targeting and interacting groups selectively locates in different sites of three pathogens and gives three naked-eye discernible emission colors. Gram-negative bacteria are weak pink, Gram-positive bacteria are orange-red and fungi are bright yellow. Therefore, based on their distinctive fluorescence response, IQ–Cm can directly discriminate the three pathogens at the cell level under a fluorescence microscope. Furthermore, we demonstrated the feasibility of IQ–Cm as a visual probe for fast diagnosis of urinary tract infections, timely monitoring of hospital-acquired infection processes and fast detection of molds in the food field. This simple visualization strategy based on one single AIEgen provides a promising platform for rapid pathogen detection and point-of-care diagnosis. |
format | Online Article Text |
id | pubmed-8159167 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-81591672021-06-11 One stone, three birds: one AIEgen with three colors for fast differentiation of three pathogens Zhou, Chengcheng Jiang, Meijuan Du, Jian Bai, Haotian Shan, Guogang Kwok, Ryan T. K. Chau, Joe H. C. Zhang, Jun Lam, Jacky W. Y. Huang, Peng Tang, Ben Zhong Chem Sci Chemistry Visually identifying pathogens favors rapid diagnosis at the point-of-care testing level. Here, we developed a microenvironment-sensitive aggregation-induced emission luminogen (AIEgen), namely IQ–Cm, for achieving fast discrimination of Gram-negative bacteria, Gram-positive bacteria and fungi by the naked-eye. With a twisted donor–acceptor and multi-rotor structure, IQ–Cm shows twisted intramolecular charge transfer (TICT) and AIE properties with sensitive fluorescence color response to the microenvironment of pathogens. Driven by the intrinsic structural differences of pathogens, IQ–Cm with a cationic isoquinolinium moiety and a membrane-active coumarin unit as the targeting and interacting groups selectively locates in different sites of three pathogens and gives three naked-eye discernible emission colors. Gram-negative bacteria are weak pink, Gram-positive bacteria are orange-red and fungi are bright yellow. Therefore, based on their distinctive fluorescence response, IQ–Cm can directly discriminate the three pathogens at the cell level under a fluorescence microscope. Furthermore, we demonstrated the feasibility of IQ–Cm as a visual probe for fast diagnosis of urinary tract infections, timely monitoring of hospital-acquired infection processes and fast detection of molds in the food field. This simple visualization strategy based on one single AIEgen provides a promising platform for rapid pathogen detection and point-of-care diagnosis. The Royal Society of Chemistry 2020-04-14 /pmc/articles/PMC8159167/ /pubmed/34122928 http://dx.doi.org/10.1039/d0sc00256a Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/ |
spellingShingle | Chemistry Zhou, Chengcheng Jiang, Meijuan Du, Jian Bai, Haotian Shan, Guogang Kwok, Ryan T. K. Chau, Joe H. C. Zhang, Jun Lam, Jacky W. Y. Huang, Peng Tang, Ben Zhong One stone, three birds: one AIEgen with three colors for fast differentiation of three pathogens |
title | One stone, three birds: one AIEgen with three colors for fast differentiation of three pathogens |
title_full | One stone, three birds: one AIEgen with three colors for fast differentiation of three pathogens |
title_fullStr | One stone, three birds: one AIEgen with three colors for fast differentiation of three pathogens |
title_full_unstemmed | One stone, three birds: one AIEgen with three colors for fast differentiation of three pathogens |
title_short | One stone, three birds: one AIEgen with three colors for fast differentiation of three pathogens |
title_sort | one stone, three birds: one aiegen with three colors for fast differentiation of three pathogens |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8159167/ https://www.ncbi.nlm.nih.gov/pubmed/34122928 http://dx.doi.org/10.1039/d0sc00256a |
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