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An interparticle relatively motional DNA walker and its sensing application

DNA molecular machines are DNA self-assemblies that perform quasi-mechanical movement at the micro–nano scale, and have attracted increasing attention in the fields of biosensing, drug delivery and biocomputing. Herein, we report the concept and operation of an interparticle relatively motional DNA...

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Autores principales: Zhang, Hong, Xu, Xiaowen, Jiang, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8159414/
https://www.ncbi.nlm.nih.gov/pubmed/34123022
http://dx.doi.org/10.1039/d0sc00109k
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author Zhang, Hong
Xu, Xiaowen
Jiang, Wei
author_facet Zhang, Hong
Xu, Xiaowen
Jiang, Wei
author_sort Zhang, Hong
collection PubMed
description DNA molecular machines are DNA self-assemblies that perform quasi-mechanical movement at the micro–nano scale, and have attracted increasing attention in the fields of biosensing, drug delivery and biocomputing. Herein, we report the concept and operation of an interparticle relatively motional DNA walker. The walker is composed of walking particles (WPs) and track particles (TPs). The WPs and TPs are obtained by respective functionalization of locked walking strands containing DNAzyme sequences and fluorophore-labelled track strands containing substrate sequences onto gold nanoparticles (AuNPs). Triggered by the target that specifically unlocks the walking strand, the liberated walking strands cooperatively hybridize with the track strands. The track strand gets cleaved by the DNAzyme, accompanied by the fluorophore release. The adjacent walking strand on the WP subsequently hybridizes to the next track strand, inducing the relative motion of the WP around the TP. After walking along the surface of one TP, the WP can continue to interact with another TP. As a result of the improved moving freedom and area, the interparticle motional mode induces high continuity and achieves large signal accumulation. Taking Zika virus RNA fragments (ZIKV-RNA) as a model target, the DNA walker shows a high sensitivity with a detection limit of 118 pM, and can reliably detect the target in biological fluids due to the stability of its components. The constructed DNA walker provides a new type of free and robust motion mode between particles and holds potential in clinical diagnosis.
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spelling pubmed-81594142021-06-11 An interparticle relatively motional DNA walker and its sensing application Zhang, Hong Xu, Xiaowen Jiang, Wei Chem Sci Chemistry DNA molecular machines are DNA self-assemblies that perform quasi-mechanical movement at the micro–nano scale, and have attracted increasing attention in the fields of biosensing, drug delivery and biocomputing. Herein, we report the concept and operation of an interparticle relatively motional DNA walker. The walker is composed of walking particles (WPs) and track particles (TPs). The WPs and TPs are obtained by respective functionalization of locked walking strands containing DNAzyme sequences and fluorophore-labelled track strands containing substrate sequences onto gold nanoparticles (AuNPs). Triggered by the target that specifically unlocks the walking strand, the liberated walking strands cooperatively hybridize with the track strands. The track strand gets cleaved by the DNAzyme, accompanied by the fluorophore release. The adjacent walking strand on the WP subsequently hybridizes to the next track strand, inducing the relative motion of the WP around the TP. After walking along the surface of one TP, the WP can continue to interact with another TP. As a result of the improved moving freedom and area, the interparticle motional mode induces high continuity and achieves large signal accumulation. Taking Zika virus RNA fragments (ZIKV-RNA) as a model target, the DNA walker shows a high sensitivity with a detection limit of 118 pM, and can reliably detect the target in biological fluids due to the stability of its components. The constructed DNA walker provides a new type of free and robust motion mode between particles and holds potential in clinical diagnosis. The Royal Society of Chemistry 2020-06-30 /pmc/articles/PMC8159414/ /pubmed/34123022 http://dx.doi.org/10.1039/d0sc00109k Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Zhang, Hong
Xu, Xiaowen
Jiang, Wei
An interparticle relatively motional DNA walker and its sensing application
title An interparticle relatively motional DNA walker and its sensing application
title_full An interparticle relatively motional DNA walker and its sensing application
title_fullStr An interparticle relatively motional DNA walker and its sensing application
title_full_unstemmed An interparticle relatively motional DNA walker and its sensing application
title_short An interparticle relatively motional DNA walker and its sensing application
title_sort interparticle relatively motional dna walker and its sensing application
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8159414/
https://www.ncbi.nlm.nih.gov/pubmed/34123022
http://dx.doi.org/10.1039/d0sc00109k
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