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NBD-Based Environment-Sensitive Fluorescent Probes for the Human Ether-a-Go-Go–Related Gene Potassium Channel

Three environment-sensitive probes were developed for the hERG channel based on the nitrobenzoxadiazole fluorophore herein. After careful evaluation, probes M1 and M3 were found to have a high affinity for imaging the hERG channel in the cell-based experiment. Compared with other fluorescent labelin...

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Autores principales: Li, Qi, Chai, Lijuan, Dong, Gaopan, Zhang, Xiaomeng, Du, Lupei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8160426/
https://www.ncbi.nlm.nih.gov/pubmed/34055884
http://dx.doi.org/10.3389/fmolb.2021.666605
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author Li, Qi
Chai, Lijuan
Dong, Gaopan
Zhang, Xiaomeng
Du, Lupei
author_facet Li, Qi
Chai, Lijuan
Dong, Gaopan
Zhang, Xiaomeng
Du, Lupei
author_sort Li, Qi
collection PubMed
description Three environment-sensitive probes were developed for the hERG channel based on the nitrobenzoxadiazole fluorophore herein. After careful evaluation, probes M1 and M3 were found to have a high affinity for imaging the hERG channel in the cell-based experiment. Compared with other fluorescent labeling technologies (such as fluorescent proteins), these probes afford a convenient and economical method to determine hERG channel in vitro and in cellulo. Therefore, these probes are expected to be applicable for usage in physiological and pathological studies of hERG channels and have the potential to establish a screening system for hERG channels.
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spelling pubmed-81604262021-05-29 NBD-Based Environment-Sensitive Fluorescent Probes for the Human Ether-a-Go-Go–Related Gene Potassium Channel Li, Qi Chai, Lijuan Dong, Gaopan Zhang, Xiaomeng Du, Lupei Front Mol Biosci Molecular Biosciences Three environment-sensitive probes were developed for the hERG channel based on the nitrobenzoxadiazole fluorophore herein. After careful evaluation, probes M1 and M3 were found to have a high affinity for imaging the hERG channel in the cell-based experiment. Compared with other fluorescent labeling technologies (such as fluorescent proteins), these probes afford a convenient and economical method to determine hERG channel in vitro and in cellulo. Therefore, these probes are expected to be applicable for usage in physiological and pathological studies of hERG channels and have the potential to establish a screening system for hERG channels. Frontiers Media S.A. 2021-05-14 /pmc/articles/PMC8160426/ /pubmed/34055884 http://dx.doi.org/10.3389/fmolb.2021.666605 Text en Copyright © 2021 Li, Chai, Dong, Zhang and Du. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Biosciences
Li, Qi
Chai, Lijuan
Dong, Gaopan
Zhang, Xiaomeng
Du, Lupei
NBD-Based Environment-Sensitive Fluorescent Probes for the Human Ether-a-Go-Go–Related Gene Potassium Channel
title NBD-Based Environment-Sensitive Fluorescent Probes for the Human Ether-a-Go-Go–Related Gene Potassium Channel
title_full NBD-Based Environment-Sensitive Fluorescent Probes for the Human Ether-a-Go-Go–Related Gene Potassium Channel
title_fullStr NBD-Based Environment-Sensitive Fluorescent Probes for the Human Ether-a-Go-Go–Related Gene Potassium Channel
title_full_unstemmed NBD-Based Environment-Sensitive Fluorescent Probes for the Human Ether-a-Go-Go–Related Gene Potassium Channel
title_short NBD-Based Environment-Sensitive Fluorescent Probes for the Human Ether-a-Go-Go–Related Gene Potassium Channel
title_sort nbd-based environment-sensitive fluorescent probes for the human ether-a-go-go–related gene potassium channel
topic Molecular Biosciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8160426/
https://www.ncbi.nlm.nih.gov/pubmed/34055884
http://dx.doi.org/10.3389/fmolb.2021.666605
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