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Sow Vaccination with a Protein Fragment against Virulent Glaesserella (Haemophilus) parasuis Modulates Immunity Traits in Their Offspring

Glaesserella (Haemophilus) parasuis, an early colonizer of the nasal cavity in piglets, is a highly heterogeneous species, comprising both commensal and virulent strains. Virulent G. parasuis strains can cause fibrinous polyserositis called Glässer’s disease. Colostrum is a source of passive immunit...

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Autores principales: López-Serrano, Sergi, Neila-Ibáñez, Carlos, Costa-Hurtado, Mar, Mahmmod, Yasser, Martínez-Martínez, Jorge, Galindo-Cardiel, Iván José, Darji, Ayub, Rodríguez, Fernando, Sibila, Marina, Aragon, Virginia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8160652/
https://www.ncbi.nlm.nih.gov/pubmed/34065547
http://dx.doi.org/10.3390/vaccines9050534
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author López-Serrano, Sergi
Neila-Ibáñez, Carlos
Costa-Hurtado, Mar
Mahmmod, Yasser
Martínez-Martínez, Jorge
Galindo-Cardiel, Iván José
Darji, Ayub
Rodríguez, Fernando
Sibila, Marina
Aragon, Virginia
author_facet López-Serrano, Sergi
Neila-Ibáñez, Carlos
Costa-Hurtado, Mar
Mahmmod, Yasser
Martínez-Martínez, Jorge
Galindo-Cardiel, Iván José
Darji, Ayub
Rodríguez, Fernando
Sibila, Marina
Aragon, Virginia
author_sort López-Serrano, Sergi
collection PubMed
description Glaesserella (Haemophilus) parasuis, an early colonizer of the nasal cavity in piglets, is a highly heterogeneous species, comprising both commensal and virulent strains. Virulent G. parasuis strains can cause fibrinous polyserositis called Glässer’s disease. Colostrum is a source of passive immunity for young piglets. When vaccinating sows, protective antibodies are transferred to their offspring through the colostrum. Here, sow vaccination was performed with a protein fragment, F4, from the outer membrane trimeric autotransporters VtaAs exclusively found in virulent G. parasuis. Piglets were allowed to suckle for 3 weeks, following which a challenge with two virulent strains of G. parasuis was performed. A group of nonvaccinated sows and their piglets were included as a control. Antibodies against F4 were confirmed using ELISA in the vaccinated sows and their offspring before the G. parasuis challenge. Compared to the control group, F4-vaccination also resulted in an increased level of serum TGF-β both in vaccinated sows and in their offspring at early time points of life. After the challenge, a lower body temperature and a higher weight were observed in the group of piglets from vaccinated sows. One piglet from the non-vaccinated group succumbed to the infection, but no other significant differences in clinical signs were noticed. At necropsy, performed 2 weeks after the virulent challenge, the level of surfactant protein D (SP-D) in bronchoalveolar lavage was higher in the piglets from vaccinated sows. Vaccination did not inhibit the nasal colonization of the piglets by the challenge strains.
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spelling pubmed-81606522021-05-29 Sow Vaccination with a Protein Fragment against Virulent Glaesserella (Haemophilus) parasuis Modulates Immunity Traits in Their Offspring López-Serrano, Sergi Neila-Ibáñez, Carlos Costa-Hurtado, Mar Mahmmod, Yasser Martínez-Martínez, Jorge Galindo-Cardiel, Iván José Darji, Ayub Rodríguez, Fernando Sibila, Marina Aragon, Virginia Vaccines (Basel) Article Glaesserella (Haemophilus) parasuis, an early colonizer of the nasal cavity in piglets, is a highly heterogeneous species, comprising both commensal and virulent strains. Virulent G. parasuis strains can cause fibrinous polyserositis called Glässer’s disease. Colostrum is a source of passive immunity for young piglets. When vaccinating sows, protective antibodies are transferred to their offspring through the colostrum. Here, sow vaccination was performed with a protein fragment, F4, from the outer membrane trimeric autotransporters VtaAs exclusively found in virulent G. parasuis. Piglets were allowed to suckle for 3 weeks, following which a challenge with two virulent strains of G. parasuis was performed. A group of nonvaccinated sows and their piglets were included as a control. Antibodies against F4 were confirmed using ELISA in the vaccinated sows and their offspring before the G. parasuis challenge. Compared to the control group, F4-vaccination also resulted in an increased level of serum TGF-β both in vaccinated sows and in their offspring at early time points of life. After the challenge, a lower body temperature and a higher weight were observed in the group of piglets from vaccinated sows. One piglet from the non-vaccinated group succumbed to the infection, but no other significant differences in clinical signs were noticed. At necropsy, performed 2 weeks after the virulent challenge, the level of surfactant protein D (SP-D) in bronchoalveolar lavage was higher in the piglets from vaccinated sows. Vaccination did not inhibit the nasal colonization of the piglets by the challenge strains. MDPI 2021-05-20 /pmc/articles/PMC8160652/ /pubmed/34065547 http://dx.doi.org/10.3390/vaccines9050534 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
López-Serrano, Sergi
Neila-Ibáñez, Carlos
Costa-Hurtado, Mar
Mahmmod, Yasser
Martínez-Martínez, Jorge
Galindo-Cardiel, Iván José
Darji, Ayub
Rodríguez, Fernando
Sibila, Marina
Aragon, Virginia
Sow Vaccination with a Protein Fragment against Virulent Glaesserella (Haemophilus) parasuis Modulates Immunity Traits in Their Offspring
title Sow Vaccination with a Protein Fragment against Virulent Glaesserella (Haemophilus) parasuis Modulates Immunity Traits in Their Offspring
title_full Sow Vaccination with a Protein Fragment against Virulent Glaesserella (Haemophilus) parasuis Modulates Immunity Traits in Their Offspring
title_fullStr Sow Vaccination with a Protein Fragment against Virulent Glaesserella (Haemophilus) parasuis Modulates Immunity Traits in Their Offspring
title_full_unstemmed Sow Vaccination with a Protein Fragment against Virulent Glaesserella (Haemophilus) parasuis Modulates Immunity Traits in Their Offspring
title_short Sow Vaccination with a Protein Fragment against Virulent Glaesserella (Haemophilus) parasuis Modulates Immunity Traits in Their Offspring
title_sort sow vaccination with a protein fragment against virulent glaesserella (haemophilus) parasuis modulates immunity traits in their offspring
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8160652/
https://www.ncbi.nlm.nih.gov/pubmed/34065547
http://dx.doi.org/10.3390/vaccines9050534
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