The Study on Molecular Profile Changes of Pathogens via Zinc Nanocomposites Immobilization Approach

The most critical group of all includes multidrug resistant bacteria that pose a particular threat in hospitals, as they can cause severe and often deadly infections. Modern medicine still faces the difficult task of developing new agents for the effective control of bacterial-based diseases. The ta...

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Autores principales: Rogowska, Agnieszka, Railean-Plugaru, Viorica, Pomastowski, Paweł, Walczak-Skierska, Justyna, Król-Górniak, Anna, Gołębiowski, Adrian, Buszewski, Bogusław
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8160681/
https://www.ncbi.nlm.nih.gov/pubmed/34065496
http://dx.doi.org/10.3390/ijms22105395
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author Rogowska, Agnieszka
Railean-Plugaru, Viorica
Pomastowski, Paweł
Walczak-Skierska, Justyna
Król-Górniak, Anna
Gołębiowski, Adrian
Buszewski, Bogusław
author_facet Rogowska, Agnieszka
Railean-Plugaru, Viorica
Pomastowski, Paweł
Walczak-Skierska, Justyna
Król-Górniak, Anna
Gołębiowski, Adrian
Buszewski, Bogusław
author_sort Rogowska, Agnieszka
collection PubMed
description The most critical group of all includes multidrug resistant bacteria that pose a particular threat in hospitals, as they can cause severe and often deadly infections. Modern medicine still faces the difficult task of developing new agents for the effective control of bacterial-based diseases. The targeted administration of nanoparticles can enhance the efficiency of conventional pharmaceutical agents. However, the interpretation of interfaces’ interactions between nanoparticles and biological systems still remains a challenge for researchers. In fact, the current research presents a strategy for using ZnO NPs immobilization with ampicillin and tetracycline. Firstly, the study provides the mechanism of the ampicillin and tetracycline binding on the surface of ZnO NPs. Secondly, it examines the effect of non-immobilized ZnO NPs, immobilized with ampicillin (ZnONPs/AMP) and tetracycline (ZnONPs/TET), on the cells’ metabolism and morphology, based on the protein and lipid profiles. A sorption kinetics study showed that the antibiotics binding on the surface of ZnONPs depend on their structure. The efficiency of the process was definitely higher in the case of ampicillin. In addition, flow cytometry results showed that immobilized nanoparticles present a different mechanism of action. Moreover, according to the MALDI approach, the antibacterial activity mechanism of the investigated ZnO complexes is mainly based on the destruction of cell membrane integrity by lipids and proteins, which is necessary for proper cell function. Additionally, it was noticed that some of the identified changes indicate the activation of defense mechanisms by cells, leading to a decrease in the permeability of a cell’s external barriers or the synthesis of repair proteins.
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spelling pubmed-81606812021-05-29 The Study on Molecular Profile Changes of Pathogens via Zinc Nanocomposites Immobilization Approach Rogowska, Agnieszka Railean-Plugaru, Viorica Pomastowski, Paweł Walczak-Skierska, Justyna Król-Górniak, Anna Gołębiowski, Adrian Buszewski, Bogusław Int J Mol Sci Article The most critical group of all includes multidrug resistant bacteria that pose a particular threat in hospitals, as they can cause severe and often deadly infections. Modern medicine still faces the difficult task of developing new agents for the effective control of bacterial-based diseases. The targeted administration of nanoparticles can enhance the efficiency of conventional pharmaceutical agents. However, the interpretation of interfaces’ interactions between nanoparticles and biological systems still remains a challenge for researchers. In fact, the current research presents a strategy for using ZnO NPs immobilization with ampicillin and tetracycline. Firstly, the study provides the mechanism of the ampicillin and tetracycline binding on the surface of ZnO NPs. Secondly, it examines the effect of non-immobilized ZnO NPs, immobilized with ampicillin (ZnONPs/AMP) and tetracycline (ZnONPs/TET), on the cells’ metabolism and morphology, based on the protein and lipid profiles. A sorption kinetics study showed that the antibiotics binding on the surface of ZnONPs depend on their structure. The efficiency of the process was definitely higher in the case of ampicillin. In addition, flow cytometry results showed that immobilized nanoparticles present a different mechanism of action. Moreover, according to the MALDI approach, the antibacterial activity mechanism of the investigated ZnO complexes is mainly based on the destruction of cell membrane integrity by lipids and proteins, which is necessary for proper cell function. Additionally, it was noticed that some of the identified changes indicate the activation of defense mechanisms by cells, leading to a decrease in the permeability of a cell’s external barriers or the synthesis of repair proteins. MDPI 2021-05-20 /pmc/articles/PMC8160681/ /pubmed/34065496 http://dx.doi.org/10.3390/ijms22105395 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rogowska, Agnieszka
Railean-Plugaru, Viorica
Pomastowski, Paweł
Walczak-Skierska, Justyna
Król-Górniak, Anna
Gołębiowski, Adrian
Buszewski, Bogusław
The Study on Molecular Profile Changes of Pathogens via Zinc Nanocomposites Immobilization Approach
title The Study on Molecular Profile Changes of Pathogens via Zinc Nanocomposites Immobilization Approach
title_full The Study on Molecular Profile Changes of Pathogens via Zinc Nanocomposites Immobilization Approach
title_fullStr The Study on Molecular Profile Changes of Pathogens via Zinc Nanocomposites Immobilization Approach
title_full_unstemmed The Study on Molecular Profile Changes of Pathogens via Zinc Nanocomposites Immobilization Approach
title_short The Study on Molecular Profile Changes of Pathogens via Zinc Nanocomposites Immobilization Approach
title_sort study on molecular profile changes of pathogens via zinc nanocomposites immobilization approach
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8160681/
https://www.ncbi.nlm.nih.gov/pubmed/34065496
http://dx.doi.org/10.3390/ijms22105395
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