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Chemical Profiling and Antimicrobial Properties of Honey Bee (Apis mellifera L.) Venom

The incidence of antibiotic resistance in pathogenic bacteria has become an alarming clinical and social problem. Therefore, the demand for alternative antimicrobial compounds has increased. In this study, a chemical profile of honey bee (Apis mellifera L.) venom (HBV) has been determined by HPLC an...

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Autores principales: Tanuwidjaja, Irina, Svečnjak, Lidija, Gugić, Domenika, Levanić, Marko, Jurić, Slaven, Vinceković, Marko, Mrkonjić Fuka, Mirna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8160683/
https://www.ncbi.nlm.nih.gov/pubmed/34065282
http://dx.doi.org/10.3390/molecules26103049
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author Tanuwidjaja, Irina
Svečnjak, Lidija
Gugić, Domenika
Levanić, Marko
Jurić, Slaven
Vinceković, Marko
Mrkonjić Fuka, Mirna
author_facet Tanuwidjaja, Irina
Svečnjak, Lidija
Gugić, Domenika
Levanić, Marko
Jurić, Slaven
Vinceković, Marko
Mrkonjić Fuka, Mirna
author_sort Tanuwidjaja, Irina
collection PubMed
description The incidence of antibiotic resistance in pathogenic bacteria has become an alarming clinical and social problem. Therefore, the demand for alternative antimicrobial compounds has increased. In this study, a chemical profile of honey bee (Apis mellifera L.) venom (HBV) has been determined by HPLC and FTIR-ATR spectroscopy, and tested for antibacterial activity, as well as efficiency with regard to conventional antibiotics. The investigated HBV was of high quality with melittin and total protein contents of 70.10 ± 7.01%, and 84.44 ± 3.12 g/100 g, respectively. The purity of HBV was confirmed by FTIR-ATR spectral profiling, which revealed a unique pattern of absorption bands that are characteristic of its major fractions. In addition, HBV showed a broad spectrum of activity against all three tested biomasses of potentially pathogenic Gram-positive and Gram-negative bacteria with MIC values ranging between 12.5 and 200 µg/mL, and MBC between 12.5 and 400 µg/mL. When compared to conventional antibiotics, HBV (400 µg) showed up to 27.8% efficiency of tetracycline (30 µg), 52.2% erythromycin (15 µg), 21.2% ciprofloxacin (5 µg), and 34.6% of ampicillin-sulbactam (20 µg). The overall results demonstrate the therapeutic potential of the analyzed HBV.
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spelling pubmed-81606832021-05-29 Chemical Profiling and Antimicrobial Properties of Honey Bee (Apis mellifera L.) Venom Tanuwidjaja, Irina Svečnjak, Lidija Gugić, Domenika Levanić, Marko Jurić, Slaven Vinceković, Marko Mrkonjić Fuka, Mirna Molecules Article The incidence of antibiotic resistance in pathogenic bacteria has become an alarming clinical and social problem. Therefore, the demand for alternative antimicrobial compounds has increased. In this study, a chemical profile of honey bee (Apis mellifera L.) venom (HBV) has been determined by HPLC and FTIR-ATR spectroscopy, and tested for antibacterial activity, as well as efficiency with regard to conventional antibiotics. The investigated HBV was of high quality with melittin and total protein contents of 70.10 ± 7.01%, and 84.44 ± 3.12 g/100 g, respectively. The purity of HBV was confirmed by FTIR-ATR spectral profiling, which revealed a unique pattern of absorption bands that are characteristic of its major fractions. In addition, HBV showed a broad spectrum of activity against all three tested biomasses of potentially pathogenic Gram-positive and Gram-negative bacteria with MIC values ranging between 12.5 and 200 µg/mL, and MBC between 12.5 and 400 µg/mL. When compared to conventional antibiotics, HBV (400 µg) showed up to 27.8% efficiency of tetracycline (30 µg), 52.2% erythromycin (15 µg), 21.2% ciprofloxacin (5 µg), and 34.6% of ampicillin-sulbactam (20 µg). The overall results demonstrate the therapeutic potential of the analyzed HBV. MDPI 2021-05-20 /pmc/articles/PMC8160683/ /pubmed/34065282 http://dx.doi.org/10.3390/molecules26103049 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tanuwidjaja, Irina
Svečnjak, Lidija
Gugić, Domenika
Levanić, Marko
Jurić, Slaven
Vinceković, Marko
Mrkonjić Fuka, Mirna
Chemical Profiling and Antimicrobial Properties of Honey Bee (Apis mellifera L.) Venom
title Chemical Profiling and Antimicrobial Properties of Honey Bee (Apis mellifera L.) Venom
title_full Chemical Profiling and Antimicrobial Properties of Honey Bee (Apis mellifera L.) Venom
title_fullStr Chemical Profiling and Antimicrobial Properties of Honey Bee (Apis mellifera L.) Venom
title_full_unstemmed Chemical Profiling and Antimicrobial Properties of Honey Bee (Apis mellifera L.) Venom
title_short Chemical Profiling and Antimicrobial Properties of Honey Bee (Apis mellifera L.) Venom
title_sort chemical profiling and antimicrobial properties of honey bee (apis mellifera l.) venom
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8160683/
https://www.ncbi.nlm.nih.gov/pubmed/34065282
http://dx.doi.org/10.3390/molecules26103049
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