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Cytotoxic Effect of Graphene Oxide Nanoribbons on Escherichia coli

The biological and environmental toxicity of graphene and graphene derivatives have attracted great research interest due to their increasing applications. However, the cytotoxic mechanism is poorly understood. Here, we investigated the cytotoxic effect of graphene oxide nanoribbons (GORs) on Escher...

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Autores principales: Qiang, Shirong, Li, Zhengbin, Zhang, Li, Luo, Dongxia, Geng, Rongyue, Zeng, Xueli, Liang, Jianjun, Li, Ping, Fan, Qiaohui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8160729/
https://www.ncbi.nlm.nih.gov/pubmed/34069641
http://dx.doi.org/10.3390/nano11051339
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author Qiang, Shirong
Li, Zhengbin
Zhang, Li
Luo, Dongxia
Geng, Rongyue
Zeng, Xueli
Liang, Jianjun
Li, Ping
Fan, Qiaohui
author_facet Qiang, Shirong
Li, Zhengbin
Zhang, Li
Luo, Dongxia
Geng, Rongyue
Zeng, Xueli
Liang, Jianjun
Li, Ping
Fan, Qiaohui
author_sort Qiang, Shirong
collection PubMed
description The biological and environmental toxicity of graphene and graphene derivatives have attracted great research interest due to their increasing applications. However, the cytotoxic mechanism is poorly understood. Here, we investigated the cytotoxic effect of graphene oxide nanoribbons (GORs) on Escherichia coli (E. coli) in an in vitro method. The fabricated GORs formed long ribbons, 200 nm wide. Based on the results of the MTT assay and plate-culture experiments, GORs significantly inhibited the growth and reproduction of E. coli in a concentration-dependent manner. We found that GORs stimulated E. coli to secrete reactive oxygen species, which then oxidized and damaged the bacterial cell membrane. Moreover, interaction between GORs and E. coli cytomembrane resulted in polysaccharide adsorption by GORs and the release of lactic dehydrogenase. Furthermore, GORs effectively depleted the metal ions as nutrients in the culture medium by adsorption. Notably, mechanical cutting by GORs was not obvious, which is quite different from the case of graphene oxide sheets to E. coli.
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spelling pubmed-81607292021-05-29 Cytotoxic Effect of Graphene Oxide Nanoribbons on Escherichia coli Qiang, Shirong Li, Zhengbin Zhang, Li Luo, Dongxia Geng, Rongyue Zeng, Xueli Liang, Jianjun Li, Ping Fan, Qiaohui Nanomaterials (Basel) Article The biological and environmental toxicity of graphene and graphene derivatives have attracted great research interest due to their increasing applications. However, the cytotoxic mechanism is poorly understood. Here, we investigated the cytotoxic effect of graphene oxide nanoribbons (GORs) on Escherichia coli (E. coli) in an in vitro method. The fabricated GORs formed long ribbons, 200 nm wide. Based on the results of the MTT assay and plate-culture experiments, GORs significantly inhibited the growth and reproduction of E. coli in a concentration-dependent manner. We found that GORs stimulated E. coli to secrete reactive oxygen species, which then oxidized and damaged the bacterial cell membrane. Moreover, interaction between GORs and E. coli cytomembrane resulted in polysaccharide adsorption by GORs and the release of lactic dehydrogenase. Furthermore, GORs effectively depleted the metal ions as nutrients in the culture medium by adsorption. Notably, mechanical cutting by GORs was not obvious, which is quite different from the case of graphene oxide sheets to E. coli. MDPI 2021-05-19 /pmc/articles/PMC8160729/ /pubmed/34069641 http://dx.doi.org/10.3390/nano11051339 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Qiang, Shirong
Li, Zhengbin
Zhang, Li
Luo, Dongxia
Geng, Rongyue
Zeng, Xueli
Liang, Jianjun
Li, Ping
Fan, Qiaohui
Cytotoxic Effect of Graphene Oxide Nanoribbons on Escherichia coli
title Cytotoxic Effect of Graphene Oxide Nanoribbons on Escherichia coli
title_full Cytotoxic Effect of Graphene Oxide Nanoribbons on Escherichia coli
title_fullStr Cytotoxic Effect of Graphene Oxide Nanoribbons on Escherichia coli
title_full_unstemmed Cytotoxic Effect of Graphene Oxide Nanoribbons on Escherichia coli
title_short Cytotoxic Effect of Graphene Oxide Nanoribbons on Escherichia coli
title_sort cytotoxic effect of graphene oxide nanoribbons on escherichia coli
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8160729/
https://www.ncbi.nlm.nih.gov/pubmed/34069641
http://dx.doi.org/10.3390/nano11051339
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