Cargando…

Experimental Evaluation of an Interferometric Light Microscopy Particle Counter for Titering and Characterization of Virus Preparations

Virus particle concentration is a critical piece of information for virology, viral vaccines and gene therapy research. We tested a novel nanoparticle counting device, “Videodrop”, for its efficacy in titering and characterization of virus particles. The Videodrop nanoparticle counter is based on in...

Descripción completa

Detalles Bibliográficos
Autores principales: Turkki, Vesa, Alppila, Elisa, Ylä-Herttuala, Seppo, Lesch, Hanna P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8160961/
https://www.ncbi.nlm.nih.gov/pubmed/34069520
http://dx.doi.org/10.3390/v13050939
_version_ 1783700400503783424
author Turkki, Vesa
Alppila, Elisa
Ylä-Herttuala, Seppo
Lesch, Hanna P.
author_facet Turkki, Vesa
Alppila, Elisa
Ylä-Herttuala, Seppo
Lesch, Hanna P.
author_sort Turkki, Vesa
collection PubMed
description Virus particle concentration is a critical piece of information for virology, viral vaccines and gene therapy research. We tested a novel nanoparticle counting device, “Videodrop”, for its efficacy in titering and characterization of virus particles. The Videodrop nanoparticle counter is based on interferometric light microscopy (ILM). The method allows the detection of particles under the diffraction limit capabilities of conventional light microscopy. We analyzed lenti-, adeno-, and baculovirus samples in different concentrations and compared the readings against traditional titering and characterization methods. The tested Videodrop particle counter is especially useful when measuring high-concentration purified virus preparations. Certain non-purified sample types or small viruses may be impossible to characterize or may require the use of standard curve or background subtraction methods, which increases the duration of the analysis. Together, our testing shows that Videodrop is a reasonable option for virus particle counting in situations where a moderate number of samples need to be analyzed quickly.
format Online
Article
Text
id pubmed-8160961
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-81609612021-05-29 Experimental Evaluation of an Interferometric Light Microscopy Particle Counter for Titering and Characterization of Virus Preparations Turkki, Vesa Alppila, Elisa Ylä-Herttuala, Seppo Lesch, Hanna P. Viruses Article Virus particle concentration is a critical piece of information for virology, viral vaccines and gene therapy research. We tested a novel nanoparticle counting device, “Videodrop”, for its efficacy in titering and characterization of virus particles. The Videodrop nanoparticle counter is based on interferometric light microscopy (ILM). The method allows the detection of particles under the diffraction limit capabilities of conventional light microscopy. We analyzed lenti-, adeno-, and baculovirus samples in different concentrations and compared the readings against traditional titering and characterization methods. The tested Videodrop particle counter is especially useful when measuring high-concentration purified virus preparations. Certain non-purified sample types or small viruses may be impossible to characterize or may require the use of standard curve or background subtraction methods, which increases the duration of the analysis. Together, our testing shows that Videodrop is a reasonable option for virus particle counting in situations where a moderate number of samples need to be analyzed quickly. MDPI 2021-05-19 /pmc/articles/PMC8160961/ /pubmed/34069520 http://dx.doi.org/10.3390/v13050939 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Turkki, Vesa
Alppila, Elisa
Ylä-Herttuala, Seppo
Lesch, Hanna P.
Experimental Evaluation of an Interferometric Light Microscopy Particle Counter for Titering and Characterization of Virus Preparations
title Experimental Evaluation of an Interferometric Light Microscopy Particle Counter for Titering and Characterization of Virus Preparations
title_full Experimental Evaluation of an Interferometric Light Microscopy Particle Counter for Titering and Characterization of Virus Preparations
title_fullStr Experimental Evaluation of an Interferometric Light Microscopy Particle Counter for Titering and Characterization of Virus Preparations
title_full_unstemmed Experimental Evaluation of an Interferometric Light Microscopy Particle Counter for Titering and Characterization of Virus Preparations
title_short Experimental Evaluation of an Interferometric Light Microscopy Particle Counter for Titering and Characterization of Virus Preparations
title_sort experimental evaluation of an interferometric light microscopy particle counter for titering and characterization of virus preparations
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8160961/
https://www.ncbi.nlm.nih.gov/pubmed/34069520
http://dx.doi.org/10.3390/v13050939
work_keys_str_mv AT turkkivesa experimentalevaluationofaninterferometriclightmicroscopyparticlecounterfortiteringandcharacterizationofviruspreparations
AT alppilaelisa experimentalevaluationofaninterferometriclightmicroscopyparticlecounterfortiteringandcharacterizationofviruspreparations
AT ylaherttualaseppo experimentalevaluationofaninterferometriclightmicroscopyparticlecounterfortiteringandcharacterizationofviruspreparations
AT leschhannap experimentalevaluationofaninterferometriclightmicroscopyparticlecounterfortiteringandcharacterizationofviruspreparations