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What Role Might Non-Mating Receptors Play in Schizophyllum commune?

The B mating-type locus of the tetrapolar basidiomycete Schizophyllum commune encodes pheromones and pheromone receptors in multiple allelic specificities. This work adds substantial new evidence into the organization of the B mating-type loci of distantly related S. commune strains showing a high l...

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Autores principales: Wirth, Sophia, Freihorst, Daniela, Krause, Katrin, Kothe, Erika
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8161036/
https://www.ncbi.nlm.nih.gov/pubmed/34065484
http://dx.doi.org/10.3390/jof7050399
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author Wirth, Sophia
Freihorst, Daniela
Krause, Katrin
Kothe, Erika
author_facet Wirth, Sophia
Freihorst, Daniela
Krause, Katrin
Kothe, Erika
author_sort Wirth, Sophia
collection PubMed
description The B mating-type locus of the tetrapolar basidiomycete Schizophyllum commune encodes pheromones and pheromone receptors in multiple allelic specificities. This work adds substantial new evidence into the organization of the B mating-type loci of distantly related S. commune strains showing a high level of synteny in gene order and neighboring genes. Four pheromone receptor-like genes were found in the genome of S. commune with brl1, brl2 and brl3 located at the B mating-type locus, whereas brl4 is located separately. Expression analysis of brl genes in different developmental stages indicates a function in filamentous growth and mating. Based on the extensive sequence analysis and functional characterization of brl-overexpression mutants, a function of Brl1 in mating is proposed, while Brl3, Brl4 and Brl2 (to a lower extent) have a role in vegetative growth, possible determination of growth direction. The brl3 and brl4 overexpression mutants had a dikaryon-like, irregular and feathery phenotype, and they avoided the formation of same-clone colonies on solid medium, which points towards enhanced detection of self-signals. These data are supported by localization of Brl fusion proteins in tips, at septa and in not-yet-fused clamps of a dikaryon, confirming their importance for growth and development in S. commune.
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spelling pubmed-81610362021-05-29 What Role Might Non-Mating Receptors Play in Schizophyllum commune? Wirth, Sophia Freihorst, Daniela Krause, Katrin Kothe, Erika J Fungi (Basel) Article The B mating-type locus of the tetrapolar basidiomycete Schizophyllum commune encodes pheromones and pheromone receptors in multiple allelic specificities. This work adds substantial new evidence into the organization of the B mating-type loci of distantly related S. commune strains showing a high level of synteny in gene order and neighboring genes. Four pheromone receptor-like genes were found in the genome of S. commune with brl1, brl2 and brl3 located at the B mating-type locus, whereas brl4 is located separately. Expression analysis of brl genes in different developmental stages indicates a function in filamentous growth and mating. Based on the extensive sequence analysis and functional characterization of brl-overexpression mutants, a function of Brl1 in mating is proposed, while Brl3, Brl4 and Brl2 (to a lower extent) have a role in vegetative growth, possible determination of growth direction. The brl3 and brl4 overexpression mutants had a dikaryon-like, irregular and feathery phenotype, and they avoided the formation of same-clone colonies on solid medium, which points towards enhanced detection of self-signals. These data are supported by localization of Brl fusion proteins in tips, at septa and in not-yet-fused clamps of a dikaryon, confirming their importance for growth and development in S. commune. MDPI 2021-05-20 /pmc/articles/PMC8161036/ /pubmed/34065484 http://dx.doi.org/10.3390/jof7050399 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wirth, Sophia
Freihorst, Daniela
Krause, Katrin
Kothe, Erika
What Role Might Non-Mating Receptors Play in Schizophyllum commune?
title What Role Might Non-Mating Receptors Play in Schizophyllum commune?
title_full What Role Might Non-Mating Receptors Play in Schizophyllum commune?
title_fullStr What Role Might Non-Mating Receptors Play in Schizophyllum commune?
title_full_unstemmed What Role Might Non-Mating Receptors Play in Schizophyllum commune?
title_short What Role Might Non-Mating Receptors Play in Schizophyllum commune?
title_sort what role might non-mating receptors play in schizophyllum commune?
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8161036/
https://www.ncbi.nlm.nih.gov/pubmed/34065484
http://dx.doi.org/10.3390/jof7050399
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