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Toxoplasma gondii Proteasome Subunit Alpha Type 1 with Chitosan: A Promising Alternative to Traditional Adjuvant

As an important zoonotic protozoan, Toxoplasma gondii (T. gondii) has spread around the world, leading to infections in one-third of the population. There is still no effective vaccine or medicine against T. gondii, and recombinant antigens entrapped within nanospheres have benefits over traditional...

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Autores principales: Yu, Zhengqing, Ding, Wenxi, Aleem, Muhammad Tahir, Su, Junzhi, Liu, Junlong, Luo, Jianxun, Yan, Ruofeng, Xu, Lixin, Song, Xiaokai, Li, Xiangrui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8161231/
https://www.ncbi.nlm.nih.gov/pubmed/34069589
http://dx.doi.org/10.3390/pharmaceutics13050752
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author Yu, Zhengqing
Ding, Wenxi
Aleem, Muhammad Tahir
Su, Junzhi
Liu, Junlong
Luo, Jianxun
Yan, Ruofeng
Xu, Lixin
Song, Xiaokai
Li, Xiangrui
author_facet Yu, Zhengqing
Ding, Wenxi
Aleem, Muhammad Tahir
Su, Junzhi
Liu, Junlong
Luo, Jianxun
Yan, Ruofeng
Xu, Lixin
Song, Xiaokai
Li, Xiangrui
author_sort Yu, Zhengqing
collection PubMed
description As an important zoonotic protozoan, Toxoplasma gondii (T. gondii) has spread around the world, leading to infections in one-third of the population. There is still no effective vaccine or medicine against T. gondii, and recombinant antigens entrapped within nanospheres have benefits over traditional vaccines. In the present study, we first expressed and purified T. gondii proteasome subunit alpha type 1 (TgPSA1), then encapsulated the recombinant TgPSA1 (rTgPSA1) in chitosan nanospheres (CS nanospheres, rTgPSA1/CS nanospheres) and incomplete Freund’s adjuvant (IFA, rTgPSA1/IFA emulsion). Antigens entrapped in CS nanospheres reached an encapsulation efficiency of 67.39%, and rTgPSA1/CS nanospheres showed a more stable release profile compared to rTgPSA1/IFA emulsion in vitro. In vivo, Th1-biased cellular and humoral immune responses were induced in mice and chickens immunized with rTgPSA1/CS nanospheres and rTgPSA1/IFA emulsion, accompanied by promoted production of antibodies, IFN-γ, IL-4, and IL-17, and modulated production of IL-10. Immunization with rTgPSA1/CS nanospheres and rTgPSA1/IFA emulsion conferred significant protection, with prolonged survival time in mice and significantly decreased parasite burden in chickens. Furthermore, our results also indicate that rTgPSA1/CS nanospheres could be used as a substitute for rTgPSA1/IFA emulsion, with the optimal administration route being intramuscular in mass vaccination. Collectively, the results of this study indicate that rTgPSA1/CS nanospheres represent a promising vaccine to protect animals against acute toxoplasmosis.
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spelling pubmed-81612312021-05-29 Toxoplasma gondii Proteasome Subunit Alpha Type 1 with Chitosan: A Promising Alternative to Traditional Adjuvant Yu, Zhengqing Ding, Wenxi Aleem, Muhammad Tahir Su, Junzhi Liu, Junlong Luo, Jianxun Yan, Ruofeng Xu, Lixin Song, Xiaokai Li, Xiangrui Pharmaceutics Article As an important zoonotic protozoan, Toxoplasma gondii (T. gondii) has spread around the world, leading to infections in one-third of the population. There is still no effective vaccine or medicine against T. gondii, and recombinant antigens entrapped within nanospheres have benefits over traditional vaccines. In the present study, we first expressed and purified T. gondii proteasome subunit alpha type 1 (TgPSA1), then encapsulated the recombinant TgPSA1 (rTgPSA1) in chitosan nanospheres (CS nanospheres, rTgPSA1/CS nanospheres) and incomplete Freund’s adjuvant (IFA, rTgPSA1/IFA emulsion). Antigens entrapped in CS nanospheres reached an encapsulation efficiency of 67.39%, and rTgPSA1/CS nanospheres showed a more stable release profile compared to rTgPSA1/IFA emulsion in vitro. In vivo, Th1-biased cellular and humoral immune responses were induced in mice and chickens immunized with rTgPSA1/CS nanospheres and rTgPSA1/IFA emulsion, accompanied by promoted production of antibodies, IFN-γ, IL-4, and IL-17, and modulated production of IL-10. Immunization with rTgPSA1/CS nanospheres and rTgPSA1/IFA emulsion conferred significant protection, with prolonged survival time in mice and significantly decreased parasite burden in chickens. Furthermore, our results also indicate that rTgPSA1/CS nanospheres could be used as a substitute for rTgPSA1/IFA emulsion, with the optimal administration route being intramuscular in mass vaccination. Collectively, the results of this study indicate that rTgPSA1/CS nanospheres represent a promising vaccine to protect animals against acute toxoplasmosis. MDPI 2021-05-19 /pmc/articles/PMC8161231/ /pubmed/34069589 http://dx.doi.org/10.3390/pharmaceutics13050752 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yu, Zhengqing
Ding, Wenxi
Aleem, Muhammad Tahir
Su, Junzhi
Liu, Junlong
Luo, Jianxun
Yan, Ruofeng
Xu, Lixin
Song, Xiaokai
Li, Xiangrui
Toxoplasma gondii Proteasome Subunit Alpha Type 1 with Chitosan: A Promising Alternative to Traditional Adjuvant
title Toxoplasma gondii Proteasome Subunit Alpha Type 1 with Chitosan: A Promising Alternative to Traditional Adjuvant
title_full Toxoplasma gondii Proteasome Subunit Alpha Type 1 with Chitosan: A Promising Alternative to Traditional Adjuvant
title_fullStr Toxoplasma gondii Proteasome Subunit Alpha Type 1 with Chitosan: A Promising Alternative to Traditional Adjuvant
title_full_unstemmed Toxoplasma gondii Proteasome Subunit Alpha Type 1 with Chitosan: A Promising Alternative to Traditional Adjuvant
title_short Toxoplasma gondii Proteasome Subunit Alpha Type 1 with Chitosan: A Promising Alternative to Traditional Adjuvant
title_sort toxoplasma gondii proteasome subunit alpha type 1 with chitosan: a promising alternative to traditional adjuvant
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8161231/
https://www.ncbi.nlm.nih.gov/pubmed/34069589
http://dx.doi.org/10.3390/pharmaceutics13050752
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