Cargando…

Metabolic Disturbances in Rat Sublines with Constitutionally Altered Serotonin Homeostasis

Central and peripheral serotonin (5HT) have opposing functions in the regulation of energy homeostasis. Both increasing 5HT signaling in the brain and decreasing 5HT signaling in the periphery have been proposed as potential treatments for obesity. This study investigates the relationship between co...

Descripción completa

Detalles Bibliográficos
Autores principales: Kesić, Maja, Baković, Petra, Stojković, Ranko, Štefulj, Jasminka, Čičin-Šain, Lipa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8161318/
https://www.ncbi.nlm.nih.gov/pubmed/34065591
http://dx.doi.org/10.3390/ijms22105400
_version_ 1783700482250768384
author Kesić, Maja
Baković, Petra
Stojković, Ranko
Štefulj, Jasminka
Čičin-Šain, Lipa
author_facet Kesić, Maja
Baković, Petra
Stojković, Ranko
Štefulj, Jasminka
Čičin-Šain, Lipa
author_sort Kesić, Maja
collection PubMed
description Central and peripheral serotonin (5HT) have opposing functions in the regulation of energy homeostasis. Both increasing 5HT signaling in the brain and decreasing 5HT signaling in the periphery have been proposed as potential treatments for obesity. This study investigates the relationship between constitutionally high or low 5HT activity and systemic net energy balance. Two sublines of rats with high and low whole-body 5HT tone, obtained by selective breeding for platelet 5HT parameters, were examined for fat accumulation in different white adipose tissue (WAT) depots, glucose/insulin tolerance, blood metabolic parameters, and expression of various metabolic genes. High-5HT animals, unlike their low-5HT counterparts, developed widespread intra-abdominal obesity associated with glucose and insulin intolerance, which worsened with age. They also had elevated blood glucose and lipid parameters but showed no significant changes in circulating leptin, resistin, and adipsin levels. Surprisingly, adiponectin levels were increased in plasma but reduced in the WAT of high-5HT rats. A limited number of metabolic genes belonging to different functional classes showed differential expression in WAT of high-5HT compared to low-5HT rats. Overall, a constitutive increase in 5HT tone is associated with a positive energy balance acting through subtle dysregulation of a broad spectrum of metabolic pathways.
format Online
Article
Text
id pubmed-8161318
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-81613182021-05-29 Metabolic Disturbances in Rat Sublines with Constitutionally Altered Serotonin Homeostasis Kesić, Maja Baković, Petra Stojković, Ranko Štefulj, Jasminka Čičin-Šain, Lipa Int J Mol Sci Article Central and peripheral serotonin (5HT) have opposing functions in the regulation of energy homeostasis. Both increasing 5HT signaling in the brain and decreasing 5HT signaling in the periphery have been proposed as potential treatments for obesity. This study investigates the relationship between constitutionally high or low 5HT activity and systemic net energy balance. Two sublines of rats with high and low whole-body 5HT tone, obtained by selective breeding for platelet 5HT parameters, were examined for fat accumulation in different white adipose tissue (WAT) depots, glucose/insulin tolerance, blood metabolic parameters, and expression of various metabolic genes. High-5HT animals, unlike their low-5HT counterparts, developed widespread intra-abdominal obesity associated with glucose and insulin intolerance, which worsened with age. They also had elevated blood glucose and lipid parameters but showed no significant changes in circulating leptin, resistin, and adipsin levels. Surprisingly, adiponectin levels were increased in plasma but reduced in the WAT of high-5HT rats. A limited number of metabolic genes belonging to different functional classes showed differential expression in WAT of high-5HT compared to low-5HT rats. Overall, a constitutive increase in 5HT tone is associated with a positive energy balance acting through subtle dysregulation of a broad spectrum of metabolic pathways. MDPI 2021-05-20 /pmc/articles/PMC8161318/ /pubmed/34065591 http://dx.doi.org/10.3390/ijms22105400 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kesić, Maja
Baković, Petra
Stojković, Ranko
Štefulj, Jasminka
Čičin-Šain, Lipa
Metabolic Disturbances in Rat Sublines with Constitutionally Altered Serotonin Homeostasis
title Metabolic Disturbances in Rat Sublines with Constitutionally Altered Serotonin Homeostasis
title_full Metabolic Disturbances in Rat Sublines with Constitutionally Altered Serotonin Homeostasis
title_fullStr Metabolic Disturbances in Rat Sublines with Constitutionally Altered Serotonin Homeostasis
title_full_unstemmed Metabolic Disturbances in Rat Sublines with Constitutionally Altered Serotonin Homeostasis
title_short Metabolic Disturbances in Rat Sublines with Constitutionally Altered Serotonin Homeostasis
title_sort metabolic disturbances in rat sublines with constitutionally altered serotonin homeostasis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8161318/
https://www.ncbi.nlm.nih.gov/pubmed/34065591
http://dx.doi.org/10.3390/ijms22105400
work_keys_str_mv AT kesicmaja metabolicdisturbancesinratsublineswithconstitutionallyalteredserotoninhomeostasis
AT bakovicpetra metabolicdisturbancesinratsublineswithconstitutionallyalteredserotoninhomeostasis
AT stojkovicranko metabolicdisturbancesinratsublineswithconstitutionallyalteredserotoninhomeostasis
AT stefuljjasminka metabolicdisturbancesinratsublineswithconstitutionallyalteredserotoninhomeostasis
AT cicinsainlipa metabolicdisturbancesinratsublineswithconstitutionallyalteredserotoninhomeostasis