LINC01158 works as an oncogene in glioma via sponging miR-6734-3p to boost CENPK expression

BACKGROUND: Long non-coding RNAs (lncRNAs) have been certified to play vital biological functions in glioma and have received considerable attention in the recent literature. Nonetheless, the role of LINC01158 in glioma remains to be elucidated. METHODS: qRT-PCR, western blot and GEPIA database were...

Descripción completa

Detalles Bibliográficos
Autores principales: Sun, Zhenxing, Wei, Naili, Yao, Shenglian, Wang, Guihuai, Sun, Yaxing, Wang, Zhenze, Yuan, Dan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Primary Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8161569/
https://www.ncbi.nlm.nih.gov/pubmed/34044826
http://dx.doi.org/10.1186/s12935-021-01931-x
_version_ 1783700536540790784
author Sun, Zhenxing
Wei, Naili
Yao, Shenglian
Wang, Guihuai
Sun, Yaxing
Wang, Zhenze
Yuan, Dan
author_facet Sun, Zhenxing
Wei, Naili
Yao, Shenglian
Wang, Guihuai
Sun, Yaxing
Wang, Zhenze
Yuan, Dan
author_sort Sun, Zhenxing
collection PubMed
description BACKGROUND: Long non-coding RNAs (lncRNAs) have been certified to play vital biological functions in glioma and have received considerable attention in the recent literature. Nonetheless, the role of LINC01158 in glioma remains to be elucidated. METHODS: qRT-PCR, western blot and GEPIA database were applied for reporting the expression of CENPK and LINC01158 in glioma and the correlation between LINC01158 and CENPK expression. EdU, colony formation, CCK-8, caspase-3 activity and TUNEL assays probed the impacts of LINC01158 on glioma cell growth. Subcellular fractionation and FISH assays revealed the cellular distribution of LINC01158. Luciferase reporter and RIP assays examined ceRNA network of LINC01158, CENPK and miR-6734-3p. RESULTS: LINC01158 and CENPK were both overexpressed in glioma and a positive regulation of LINC01158 on CENPK was corroborated. LINC01158 served a pro-proliferative and anti-apoptotic part in glioma by sponging miR-6734-3p to augment CENPK. CONCLUSION: LINC01158 enhances CENPK by serving as sponge for miR-6734-3p to facilitate glioma development, proposing LINC01158 as a new player in glioma. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12935-021-01931-x.
format Online
Article
Text
id pubmed-8161569
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-81615692021-06-01 LINC01158 works as an oncogene in glioma via sponging miR-6734-3p to boost CENPK expression Sun, Zhenxing Wei, Naili Yao, Shenglian Wang, Guihuai Sun, Yaxing Wang, Zhenze Yuan, Dan Cancer Cell Int Primary Research BACKGROUND: Long non-coding RNAs (lncRNAs) have been certified to play vital biological functions in glioma and have received considerable attention in the recent literature. Nonetheless, the role of LINC01158 in glioma remains to be elucidated. METHODS: qRT-PCR, western blot and GEPIA database were applied for reporting the expression of CENPK and LINC01158 in glioma and the correlation between LINC01158 and CENPK expression. EdU, colony formation, CCK-8, caspase-3 activity and TUNEL assays probed the impacts of LINC01158 on glioma cell growth. Subcellular fractionation and FISH assays revealed the cellular distribution of LINC01158. Luciferase reporter and RIP assays examined ceRNA network of LINC01158, CENPK and miR-6734-3p. RESULTS: LINC01158 and CENPK were both overexpressed in glioma and a positive regulation of LINC01158 on CENPK was corroborated. LINC01158 served a pro-proliferative and anti-apoptotic part in glioma by sponging miR-6734-3p to augment CENPK. CONCLUSION: LINC01158 enhances CENPK by serving as sponge for miR-6734-3p to facilitate glioma development, proposing LINC01158 as a new player in glioma. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12935-021-01931-x. BioMed Central 2021-05-27 /pmc/articles/PMC8161569/ /pubmed/34044826 http://dx.doi.org/10.1186/s12935-021-01931-x Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Primary Research
Sun, Zhenxing
Wei, Naili
Yao, Shenglian
Wang, Guihuai
Sun, Yaxing
Wang, Zhenze
Yuan, Dan
LINC01158 works as an oncogene in glioma via sponging miR-6734-3p to boost CENPK expression
title LINC01158 works as an oncogene in glioma via sponging miR-6734-3p to boost CENPK expression
title_full LINC01158 works as an oncogene in glioma via sponging miR-6734-3p to boost CENPK expression
title_fullStr LINC01158 works as an oncogene in glioma via sponging miR-6734-3p to boost CENPK expression
title_full_unstemmed LINC01158 works as an oncogene in glioma via sponging miR-6734-3p to boost CENPK expression
title_short LINC01158 works as an oncogene in glioma via sponging miR-6734-3p to boost CENPK expression
title_sort linc01158 works as an oncogene in glioma via sponging mir-6734-3p to boost cenpk expression
topic Primary Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8161569/
https://www.ncbi.nlm.nih.gov/pubmed/34044826
http://dx.doi.org/10.1186/s12935-021-01931-x
work_keys_str_mv AT sunzhenxing linc01158worksasanoncogeneingliomaviaspongingmir67343ptoboostcenpkexpression
AT weinaili linc01158worksasanoncogeneingliomaviaspongingmir67343ptoboostcenpkexpression
AT yaoshenglian linc01158worksasanoncogeneingliomaviaspongingmir67343ptoboostcenpkexpression
AT wangguihuai linc01158worksasanoncogeneingliomaviaspongingmir67343ptoboostcenpkexpression
AT sunyaxing linc01158worksasanoncogeneingliomaviaspongingmir67343ptoboostcenpkexpression
AT wangzhenze linc01158worksasanoncogeneingliomaviaspongingmir67343ptoboostcenpkexpression
AT yuandan linc01158worksasanoncogeneingliomaviaspongingmir67343ptoboostcenpkexpression

Ejemplares similares