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Limitations of PCR detection of filarial DNA in human stools from subjects non-infected with soil-transmitted helminths

The standard techniques for diagnosis of human filariasis are the microscopic examination of blood smears or skin biopsies, which are relatively invasive and poorly sensitive at low levels of infection. Recently, filarial DNA has been detected in fecal samples from non-human primates in Central Afri...

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Autores principales: Doret, Maxime P. M., Nana-Djeunga, Hugues C., Nzune-Toche, Narcisse, Pion, Sébastien D. S., Chesnais, Cédric B., Boussinesq, Michel, Kamgno, Joseph, Varlet-Marie, Emmanuelle, Locatelli, Sabrina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: EDP Sciences 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8162058/
https://www.ncbi.nlm.nih.gov/pubmed/34047694
http://dx.doi.org/10.1051/parasite/2021046
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author Doret, Maxime P. M.
Nana-Djeunga, Hugues C.
Nzune-Toche, Narcisse
Pion, Sébastien D. S.
Chesnais, Cédric B.
Boussinesq, Michel
Kamgno, Joseph
Varlet-Marie, Emmanuelle
Locatelli, Sabrina
author_facet Doret, Maxime P. M.
Nana-Djeunga, Hugues C.
Nzune-Toche, Narcisse
Pion, Sébastien D. S.
Chesnais, Cédric B.
Boussinesq, Michel
Kamgno, Joseph
Varlet-Marie, Emmanuelle
Locatelli, Sabrina
author_sort Doret, Maxime P. M.
collection PubMed
description The standard techniques for diagnosis of human filariasis are the microscopic examination of blood smears or skin biopsies, which are relatively invasive and poorly sensitive at low levels of infection. Recently, filarial DNA has been detected in fecal samples from non-human primates in Central Africa. The aim of this study was to demonstrate proof-of-concept of a non-invasive molecular diagnosis technique for human filariasis by targeting fragments of 12S rDNA, Cox1, ITS1 and LL20-15kDa ladder antigen-gene by conventional PCR in DNA extracted from stool samples of 52 people infected with Mansonella perstans and/or Loa loa. Of these, 10 patients were infected with soil-transmitted helminths (Trichuris trichiura and/or Ascaris lumbricoides), and none were positive for Necator americanus. Interestingly, no filarial gene fragments were detected in the stools of any of the 52 patients. Future studies should evaluate whether a co-infection with soil-transmitted helminths causing gastrointestinal bleeding and likely allowing (micro)filaria exit into the digestive tract, may facilitate the molecular detection of filarial DNA fragments in stool samples.
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spelling pubmed-81620582021-06-04 Limitations of PCR detection of filarial DNA in human stools from subjects non-infected with soil-transmitted helminths Doret, Maxime P. M. Nana-Djeunga, Hugues C. Nzune-Toche, Narcisse Pion, Sébastien D. S. Chesnais, Cédric B. Boussinesq, Michel Kamgno, Joseph Varlet-Marie, Emmanuelle Locatelli, Sabrina Parasite Research Article The standard techniques for diagnosis of human filariasis are the microscopic examination of blood smears or skin biopsies, which are relatively invasive and poorly sensitive at low levels of infection. Recently, filarial DNA has been detected in fecal samples from non-human primates in Central Africa. The aim of this study was to demonstrate proof-of-concept of a non-invasive molecular diagnosis technique for human filariasis by targeting fragments of 12S rDNA, Cox1, ITS1 and LL20-15kDa ladder antigen-gene by conventional PCR in DNA extracted from stool samples of 52 people infected with Mansonella perstans and/or Loa loa. Of these, 10 patients were infected with soil-transmitted helminths (Trichuris trichiura and/or Ascaris lumbricoides), and none were positive for Necator americanus. Interestingly, no filarial gene fragments were detected in the stools of any of the 52 patients. Future studies should evaluate whether a co-infection with soil-transmitted helminths causing gastrointestinal bleeding and likely allowing (micro)filaria exit into the digestive tract, may facilitate the molecular detection of filarial DNA fragments in stool samples. EDP Sciences 2021-05-27 /pmc/articles/PMC8162058/ /pubmed/34047694 http://dx.doi.org/10.1051/parasite/2021046 Text en © M.P.M. Doret et al., published by EDP Sciences, 2021 https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0 (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Doret, Maxime P. M.
Nana-Djeunga, Hugues C.
Nzune-Toche, Narcisse
Pion, Sébastien D. S.
Chesnais, Cédric B.
Boussinesq, Michel
Kamgno, Joseph
Varlet-Marie, Emmanuelle
Locatelli, Sabrina
Limitations of PCR detection of filarial DNA in human stools from subjects non-infected with soil-transmitted helminths
title Limitations of PCR detection of filarial DNA in human stools from subjects non-infected with soil-transmitted helminths
title_full Limitations of PCR detection of filarial DNA in human stools from subjects non-infected with soil-transmitted helminths
title_fullStr Limitations of PCR detection of filarial DNA in human stools from subjects non-infected with soil-transmitted helminths
title_full_unstemmed Limitations of PCR detection of filarial DNA in human stools from subjects non-infected with soil-transmitted helminths
title_short Limitations of PCR detection of filarial DNA in human stools from subjects non-infected with soil-transmitted helminths
title_sort limitations of pcr detection of filarial dna in human stools from subjects non-infected with soil-transmitted helminths
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8162058/
https://www.ncbi.nlm.nih.gov/pubmed/34047694
http://dx.doi.org/10.1051/parasite/2021046
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