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De Novo Sequencing Provides Insights Into the Pathogenicity of Foodborne Vibrio parahaemolyticus

Vibrio parahaemolyticus is a common pathogenic marine bacterium that causes gastrointestinal infections and other health complications, which could be life-threatening to immunocompromised patients. For the past two decades, the pathogenicity of environmental V. parahaemolyticus has increased greatl...

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Autores principales: Liu, Jianfei, Qin, Kewei, Wu, Chenglin, Fu, Kaifei, Yu, Xiaojie, Zhou, Lijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8162212/
https://www.ncbi.nlm.nih.gov/pubmed/34055666
http://dx.doi.org/10.3389/fcimb.2021.652957
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author Liu, Jianfei
Qin, Kewei
Wu, Chenglin
Fu, Kaifei
Yu, Xiaojie
Zhou, Lijun
author_facet Liu, Jianfei
Qin, Kewei
Wu, Chenglin
Fu, Kaifei
Yu, Xiaojie
Zhou, Lijun
author_sort Liu, Jianfei
collection PubMed
description Vibrio parahaemolyticus is a common pathogenic marine bacterium that causes gastrointestinal infections and other health complications, which could be life-threatening to immunocompromised patients. For the past two decades, the pathogenicity of environmental V. parahaemolyticus has increased greatly, and the genomic change behind this phenomenon still needs an in-depth exploration. To investigate the difference in pathogenicity at the genomic level, three strains with different hemolysin expression and biofilm formation capacity were screened out of 69 environmental V. parahaemolyticus strains. Subsequently, 16S rDNA analysis, de novo sequencing, pathogenicity test, and antibiotic resistance assays were performed. Comparative genome-scale interpretation showed that various functional region differences in pathogenicity of the selected V. parahaemolyticus strains were due to dissimilarities in the distribution of key genetic elements and in the secretory system compositions. Furthermore, the genomic analysis-based hypothesis of distinct pathogenic effects was verified by the survival rate of mouse models infected with different V. parahaemolyticus strains. Antibiotic resistance results also presented the multi-directional evolutionary potential in environmental V. parahaemolyticus, in agreement with the phylogenetic analysis results. Our study provides a theoretical basis for better understanding of the increasing pathogenicity of environmental V. parahaemolyticus at the genome level. Further, it has a key referential value for the exploration of pathogenicity and prevention of environmental V. parahaemolyticus in the future.
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spelling pubmed-81622122021-05-29 De Novo Sequencing Provides Insights Into the Pathogenicity of Foodborne Vibrio parahaemolyticus Liu, Jianfei Qin, Kewei Wu, Chenglin Fu, Kaifei Yu, Xiaojie Zhou, Lijun Front Cell Infect Microbiol Cellular and Infection Microbiology Vibrio parahaemolyticus is a common pathogenic marine bacterium that causes gastrointestinal infections and other health complications, which could be life-threatening to immunocompromised patients. For the past two decades, the pathogenicity of environmental V. parahaemolyticus has increased greatly, and the genomic change behind this phenomenon still needs an in-depth exploration. To investigate the difference in pathogenicity at the genomic level, three strains with different hemolysin expression and biofilm formation capacity were screened out of 69 environmental V. parahaemolyticus strains. Subsequently, 16S rDNA analysis, de novo sequencing, pathogenicity test, and antibiotic resistance assays were performed. Comparative genome-scale interpretation showed that various functional region differences in pathogenicity of the selected V. parahaemolyticus strains were due to dissimilarities in the distribution of key genetic elements and in the secretory system compositions. Furthermore, the genomic analysis-based hypothesis of distinct pathogenic effects was verified by the survival rate of mouse models infected with different V. parahaemolyticus strains. Antibiotic resistance results also presented the multi-directional evolutionary potential in environmental V. parahaemolyticus, in agreement with the phylogenetic analysis results. Our study provides a theoretical basis for better understanding of the increasing pathogenicity of environmental V. parahaemolyticus at the genome level. Further, it has a key referential value for the exploration of pathogenicity and prevention of environmental V. parahaemolyticus in the future. Frontiers Media S.A. 2021-05-14 /pmc/articles/PMC8162212/ /pubmed/34055666 http://dx.doi.org/10.3389/fcimb.2021.652957 Text en Copyright © 2021 Liu, Qin, Wu, Fu, Yu and Zhou https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Liu, Jianfei
Qin, Kewei
Wu, Chenglin
Fu, Kaifei
Yu, Xiaojie
Zhou, Lijun
De Novo Sequencing Provides Insights Into the Pathogenicity of Foodborne Vibrio parahaemolyticus
title De Novo Sequencing Provides Insights Into the Pathogenicity of Foodborne Vibrio parahaemolyticus
title_full De Novo Sequencing Provides Insights Into the Pathogenicity of Foodborne Vibrio parahaemolyticus
title_fullStr De Novo Sequencing Provides Insights Into the Pathogenicity of Foodborne Vibrio parahaemolyticus
title_full_unstemmed De Novo Sequencing Provides Insights Into the Pathogenicity of Foodborne Vibrio parahaemolyticus
title_short De Novo Sequencing Provides Insights Into the Pathogenicity of Foodborne Vibrio parahaemolyticus
title_sort de novo sequencing provides insights into the pathogenicity of foodborne vibrio parahaemolyticus
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8162212/
https://www.ncbi.nlm.nih.gov/pubmed/34055666
http://dx.doi.org/10.3389/fcimb.2021.652957
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