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Polymorphism and molecular characteristics of the CSN1S2 gene in river and swamp buffalo

The [Formula: see text]-casein ([Formula: see text]-CN) is a member of the casein family associated with milk traits in ruminants, but so far the buffalo CSN1S2 gene has not been well understood. In this work, the polymorphisms of CSN1S2 in river and swamp buffalo were detected using direct sequenci...

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Detalles Bibliográficos
Autores principales: Fan, Xinyang, Gao, Shanshan, Fu, Lin, Qiu, Lihua, Miao, Yongwang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Copernicus GmbH 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8162411/
https://www.ncbi.nlm.nih.gov/pubmed/34084897
http://dx.doi.org/10.5194/aab-63-345-2020
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author Fan, Xinyang
Gao, Shanshan
Fu, Lin
Qiu, Lihua
Miao, Yongwang
author_facet Fan, Xinyang
Gao, Shanshan
Fu, Lin
Qiu, Lihua
Miao, Yongwang
author_sort Fan, Xinyang
collection PubMed
description The [Formula: see text]-casein ([Formula: see text]-CN) is a member of the casein family associated with milk traits in ruminants, but so far the buffalo CSN1S2 gene has not been well understood. In this work, the polymorphisms of CSN1S2 in river and swamp buffalo were detected using direct sequencing of polymerase chain reaction (PCR) products. As a result, 13 single nucleotide polymorphisms (SNPs) were identified in the coding sequence (CDS) of CSN1S2 in two types of buffalo, of which eight SNPs were non-synonymous. The amino acid changes caused by c.580T [Formula: see text] C and c.642C [Formula: see text] G may affect the function of buffalo [Formula: see text]-CN. A total of 11 CSN1S2 CDS haplotypes were defined, and accordingly 11 variants of buffalo [Formula: see text]-CN were inferred and named. The CSN1S2 CDSs of both types of buffalo were 669 nucleotides, which encoded a precursor of 222 amino acids (AAs), and the first 15 AAs constitute a signal peptide. The composition and physicochemical characteristics of two types of buffalo [Formula: see text]-CNs were similar but slightly different from those of cattle [Formula: see text]-CN. The [Formula: see text]-CN mature peptides of buffalo and the species of Bos genus contained a casein domain, and their secondary structures were highly consistent, indicating that they are functionally similar. The results here provide initial insights into the variation, characteristics and biological function of buffalo CSN1S2.
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spelling pubmed-81624112021-06-02 Polymorphism and molecular characteristics of the CSN1S2 gene in river and swamp buffalo Fan, Xinyang Gao, Shanshan Fu, Lin Qiu, Lihua Miao, Yongwang Arch Anim Breed Original Study The [Formula: see text]-casein ([Formula: see text]-CN) is a member of the casein family associated with milk traits in ruminants, but so far the buffalo CSN1S2 gene has not been well understood. In this work, the polymorphisms of CSN1S2 in river and swamp buffalo were detected using direct sequencing of polymerase chain reaction (PCR) products. As a result, 13 single nucleotide polymorphisms (SNPs) were identified in the coding sequence (CDS) of CSN1S2 in two types of buffalo, of which eight SNPs were non-synonymous. The amino acid changes caused by c.580T [Formula: see text] C and c.642C [Formula: see text] G may affect the function of buffalo [Formula: see text]-CN. A total of 11 CSN1S2 CDS haplotypes were defined, and accordingly 11 variants of buffalo [Formula: see text]-CN were inferred and named. The CSN1S2 CDSs of both types of buffalo were 669 nucleotides, which encoded a precursor of 222 amino acids (AAs), and the first 15 AAs constitute a signal peptide. The composition and physicochemical characteristics of two types of buffalo [Formula: see text]-CNs were similar but slightly different from those of cattle [Formula: see text]-CN. The [Formula: see text]-CN mature peptides of buffalo and the species of Bos genus contained a casein domain, and their secondary structures were highly consistent, indicating that they are functionally similar. The results here provide initial insights into the variation, characteristics and biological function of buffalo CSN1S2. Copernicus GmbH 2020-09-18 /pmc/articles/PMC8162411/ /pubmed/34084897 http://dx.doi.org/10.5194/aab-63-345-2020 Text en Copyright: © 2020 Xinyang Fan et al. https://creativecommons.org/licenses/by/4.0/ This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/
spellingShingle Original Study
Fan, Xinyang
Gao, Shanshan
Fu, Lin
Qiu, Lihua
Miao, Yongwang
Polymorphism and molecular characteristics of the CSN1S2 gene in river and swamp buffalo
title Polymorphism and molecular characteristics of the CSN1S2 gene in river and swamp buffalo
title_full Polymorphism and molecular characteristics of the CSN1S2 gene in river and swamp buffalo
title_fullStr Polymorphism and molecular characteristics of the CSN1S2 gene in river and swamp buffalo
title_full_unstemmed Polymorphism and molecular characteristics of the CSN1S2 gene in river and swamp buffalo
title_short Polymorphism and molecular characteristics of the CSN1S2 gene in river and swamp buffalo
title_sort polymorphism and molecular characteristics of the csn1s2 gene in river and swamp buffalo
topic Original Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8162411/
https://www.ncbi.nlm.nih.gov/pubmed/34084897
http://dx.doi.org/10.5194/aab-63-345-2020
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