Cargando…
The Role of Spermidine Synthase (SpdS) and Spermine Synthase (Sms) in Regulating Triglyceride Storage in Drosophila
Polyamines are small organic cations that are important for several biological processes such as cell proliferation, cell cycle progression, and apoptosis. The dysregulation of intracellular polyamines is often associated with diseases such as cancer, diabetes, and developmental disorders. Although...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8162547/ https://www.ncbi.nlm.nih.gov/pubmed/34063217 http://dx.doi.org/10.3390/medsci9020027 |
_version_ | 1783700737910374400 |
---|---|
author | Morales, Tahj S. Avis, Erik C. Paskowski, Elise K. Shabar, Hamza Nowotarski, Shannon L. DiAngelo, Justin R. |
author_facet | Morales, Tahj S. Avis, Erik C. Paskowski, Elise K. Shabar, Hamza Nowotarski, Shannon L. DiAngelo, Justin R. |
author_sort | Morales, Tahj S. |
collection | PubMed |
description | Polyamines are small organic cations that are important for several biological processes such as cell proliferation, cell cycle progression, and apoptosis. The dysregulation of intracellular polyamines is often associated with diseases such as cancer, diabetes, and developmental disorders. Although polyamine metabolism has been well studied, the effects of key enzymes in the polyamine pathway on lipid metabolism are not well understood. Here, we determined metabolic effects resulting from the absence of spermidine synthase (SpdS) and spermine synthase (Sms) in Drosophila. While SpdS mutants developed normally and accumulated triglycerides, Sms mutants had reduced viability and stored less triglyceride than the controls. Interestingly, when decreasing SpdS and Sms, specifically in the fat body, triglyceride storage increased. While there was no difference in triglycerides stored in heads, thoraxes and abdomen fat bodies, abdomen fat body DNA content increased, and protein/DNA decreased in both SpdS- and Sms-RNAi flies, suggesting that fat body-specific knockdown of SpdS and Sms causes the production of smaller fat body cells and triglycerides to accumulate in non-fat body tissues of the abdomen. Together, these data provide support for the role that polyamines play in the regulation of metabolism and can help enhance our understanding of polyamine function in metabolic diseases. |
format | Online Article Text |
id | pubmed-8162547 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-81625472021-05-29 The Role of Spermidine Synthase (SpdS) and Spermine Synthase (Sms) in Regulating Triglyceride Storage in Drosophila Morales, Tahj S. Avis, Erik C. Paskowski, Elise K. Shabar, Hamza Nowotarski, Shannon L. DiAngelo, Justin R. Med Sci (Basel) Article Polyamines are small organic cations that are important for several biological processes such as cell proliferation, cell cycle progression, and apoptosis. The dysregulation of intracellular polyamines is often associated with diseases such as cancer, diabetes, and developmental disorders. Although polyamine metabolism has been well studied, the effects of key enzymes in the polyamine pathway on lipid metabolism are not well understood. Here, we determined metabolic effects resulting from the absence of spermidine synthase (SpdS) and spermine synthase (Sms) in Drosophila. While SpdS mutants developed normally and accumulated triglycerides, Sms mutants had reduced viability and stored less triglyceride than the controls. Interestingly, when decreasing SpdS and Sms, specifically in the fat body, triglyceride storage increased. While there was no difference in triglycerides stored in heads, thoraxes and abdomen fat bodies, abdomen fat body DNA content increased, and protein/DNA decreased in both SpdS- and Sms-RNAi flies, suggesting that fat body-specific knockdown of SpdS and Sms causes the production of smaller fat body cells and triglycerides to accumulate in non-fat body tissues of the abdomen. Together, these data provide support for the role that polyamines play in the regulation of metabolism and can help enhance our understanding of polyamine function in metabolic diseases. MDPI 2021-05-02 /pmc/articles/PMC8162547/ /pubmed/34063217 http://dx.doi.org/10.3390/medsci9020027 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Morales, Tahj S. Avis, Erik C. Paskowski, Elise K. Shabar, Hamza Nowotarski, Shannon L. DiAngelo, Justin R. The Role of Spermidine Synthase (SpdS) and Spermine Synthase (Sms) in Regulating Triglyceride Storage in Drosophila |
title | The Role of Spermidine Synthase (SpdS) and Spermine Synthase (Sms) in Regulating Triglyceride Storage in Drosophila |
title_full | The Role of Spermidine Synthase (SpdS) and Spermine Synthase (Sms) in Regulating Triglyceride Storage in Drosophila |
title_fullStr | The Role of Spermidine Synthase (SpdS) and Spermine Synthase (Sms) in Regulating Triglyceride Storage in Drosophila |
title_full_unstemmed | The Role of Spermidine Synthase (SpdS) and Spermine Synthase (Sms) in Regulating Triglyceride Storage in Drosophila |
title_short | The Role of Spermidine Synthase (SpdS) and Spermine Synthase (Sms) in Regulating Triglyceride Storage in Drosophila |
title_sort | role of spermidine synthase (spds) and spermine synthase (sms) in regulating triglyceride storage in drosophila |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8162547/ https://www.ncbi.nlm.nih.gov/pubmed/34063217 http://dx.doi.org/10.3390/medsci9020027 |
work_keys_str_mv | AT moralestahjs theroleofspermidinesynthasespdsandsperminesynthasesmsinregulatingtriglyceridestorageindrosophila AT aviserikc theroleofspermidinesynthasespdsandsperminesynthasesmsinregulatingtriglyceridestorageindrosophila AT paskowskielisek theroleofspermidinesynthasespdsandsperminesynthasesmsinregulatingtriglyceridestorageindrosophila AT shabarhamza theroleofspermidinesynthasespdsandsperminesynthasesmsinregulatingtriglyceridestorageindrosophila AT nowotarskishannonl theroleofspermidinesynthasespdsandsperminesynthasesmsinregulatingtriglyceridestorageindrosophila AT diangelojustinr theroleofspermidinesynthasespdsandsperminesynthasesmsinregulatingtriglyceridestorageindrosophila AT moralestahjs roleofspermidinesynthasespdsandsperminesynthasesmsinregulatingtriglyceridestorageindrosophila AT aviserikc roleofspermidinesynthasespdsandsperminesynthasesmsinregulatingtriglyceridestorageindrosophila AT paskowskielisek roleofspermidinesynthasespdsandsperminesynthasesmsinregulatingtriglyceridestorageindrosophila AT shabarhamza roleofspermidinesynthasespdsandsperminesynthasesmsinregulatingtriglyceridestorageindrosophila AT nowotarskishannonl roleofspermidinesynthasespdsandsperminesynthasesmsinregulatingtriglyceridestorageindrosophila AT diangelojustinr roleofspermidinesynthasespdsandsperminesynthasesmsinregulatingtriglyceridestorageindrosophila |